Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,239,202	A→G	A108A (GCA→GCG)	dadX →	alanine racemase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,239,202	0	A	G	100.0%	164.2 / NA	46	A108A (GCA→GCG)	dadX	alanine racemase 2
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/21);  total (25/21)

GCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCATGCTCAGGATCTGGAGATTTATGACCAGCACCGCCTGACCACCTGCGTACACAGCAACTGGCAGCTCAAAGCACTGCAAAATGCGCGGCTAAAAGCACCGTTGGATATTTATCTTAAAGTGAACAGTGGGATGAATCGGTTGGGCTTCCAGCCCGATCGCGTGCTTACCGTCTGGCAGCAGTTGCGGGCAATGGCGAATGTTGGCGAA  >  NC_000913/1239068‑1239337                                                                                                                                       |                                                                                                                                        gCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCATGCTCAGGATCTGGAGATTTATGACCAGCACCGCCTGACCACCTGCGTACACAGCAACTGGCAGCTCAAAGCGCTGCa                                                                                                                                    >  1:266611/1‑140 (MQ=255)         gTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCATGCTCAGGATCTGGAGATTTATGACCAGCACCGCCTGACCACCTGCGTACACAGCAACTGGCAGCTCAAAGCGCTGCAAAATgcgc                                                                                                                            <  1:331236/140‑1 (MQ=255)           tACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCATGCTCAGGATCTGGAGATTTATGACCAGCACCGCCTGACCACCTGCGTACACAGCAACTGGCAGCTCAAAGCGCTGCAAAATGCGCgg                                                                                                                          >  1:488329/1‑140 (MQ=255)             cGTGAGCGCGGCTGGAAAGGACCGATCTTGATGCTGGAAGGAGTTTTCCATGCTCAGGATCTGGAGATTTATGACCAGCACCGCCTCACCACCTGCGCACACAGCAACTCGCAGCTCAACGCGCTGCAAAATGCGCGGCt                                                                                                                        >  2:706681/1‑140 (MQ=255)               tGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCATGCTCAGGATCTGGAGATTTATGACCAGCACCGCCTGACCACCTGCGTACACAGCAACTGGCAGCTCAAAGCGCTGCAAAATGCGCGGCTaa                                                                                                                      >  2:185627/1‑140 (MQ=255)                  gcgcGGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCATGCTCAGGATCTGGAGATTTATGACCAGCACCGCCTGACCACCAGCGTACACAGCAACTGGCAGCTCAAAGCGCTGCAAAATGCGCGGCTAAAAg                                                                                                                   <  1:17160/140‑1 (MQ=255)                   cgcgGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCATGCTCAGGATCTGGAGATTTATGACCAGCACCGCCTGACCACCTGCGTACACAGCAACTGGCAGCTCAAAGCGCTGCAAAATGCGCGGCTAAAAGc                                                                                                                  >  1:16802/1‑140 (MQ=255)                    gcgGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCATGCTCAGGATCTGGAGATTTATGACCAGCACCGCCTGACCACCTGCGTACACAGCAACTGGCAGCTCAAAGCGCTGCAAAATGCGCGGCTAAAAGCa                                                                                                                 <  2:810802/140‑1 (MQ=255)                    gcgGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCATGCTCAGGATCTGGAGATTTATGACCAGCACCGCCTGACCACCTGCGTACACAGCAACTGGCAGCTCAAAGCGCTGCAAAATGCGCGGCTAAAAGCa                                                                                                                 <  1:181143/140‑1 (MQ=255)                         tGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCATGCTCAGGATCTGGAGATTTATGACCAGCACCGCCTGACCACCTGCGTACACAGCAACTGGCAGCTCAAAGCGCTGCAAAATGCGCGGCTAAAAGCACCGtt                                                                                                            <  1:605134/140‑1 (MQ=255)                           gAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCATGCTCAGGATCTGGAGATTTATGACCAGCACCGCCTGACCACCTGCGTACACAGCAACTGGCAGCTCAAAGCGCTGCAAAATGCGCGGCTAAAAGCACCGTTgg                                                                                                          <  1:781406/140‑1 (MQ=255)                                  ccGATCCTGATGCTGGAAGGATTTTTCCATGCTCAGGATCTGGAGATTTATGACCAGCACCGCCTGACCACCTGCGTACACAGCAACTGGCAGCTCAAAGCGCTGCAAAATGCGCGGCTAAAAGCACCGTTGGATATTTa                                                                                                   >  2:405929/1‑140 (MQ=255)                                   cGATCCTGATGCTGGAAGGATTTTTCCATGCTCAGGATCTGGAGATTTATGACCAGCACCGCCTGACCACCTGCGTACACAGCAACTGGCAGCTCAAAGCGCTGCAAAATGCGCGGCTAAAAGCACCGTTGGATATTTAt                                                                                                  >  2:437584/1‑140 (MQ=255)                                    gATCCTGATGCTGGAAGGATTTTTCCATGCTCAGGATCTGGAGATTTATGACCAGCACCGCCTGACCACCTGCGTACACAGCAACTGGCAGCTCAAAGCGCTGCAAAATGCGCGGCTAAAAGCACCGTTGGATATTTATc                                                                                                 <  2:511386/140‑1 (MQ=255)                                     aTCCTGATGCTGGAAGGATTTTTCCATGCTCAGGATCTGGAGATTTATGACCAGCACCGCCTGACCACCTGCGTACACAGCAACTGGCAGCTCAAAGCGCTGCAAAATGCGCGGCTAAAAGCACCGTTGGATATTTATCt                                                                                                >  1:646646/1‑140 (MQ=255)                                            tGCTGGAAGGATTTTTCCATGCTCAGGATCTGGAGATTTATGACCAGCACCGCCTGACCACCTGCGTACACAGCAACTGGCAGCTCAAAGCGCTGCAAAATGCGCGGCTAAAAGCACCGTTGGATATTTATCTTAAAGTg                                                                                         <  1:437584/140‑1 (MQ=255)                                              cTGGAAGGATTTTTCCATGCTCAGGATCTGGAGATTTATGACCAGCACCGCCTGACCACCTGCGTACACAGCAACTGGCAGCTCAAAGCGCTGCAAAATGCGCGGCTAAAAGCACCGTTGGATATTTATCTTAAAGTGaa                                                                                       <  2:266611/140‑1 (MQ=255)                                              cTGGAAGGATTTTTCCATGCTCAGGATCTGGAGATTTATGACCAGCACCGCCTGACCACCTGCGTACACAGCAACTGGCAGCTCAAAGCGCTGCAAAATGCGCGGCTAAAAGCACCGTTGGATATTTATCTTAAAGTGaa                                                                                       <  1:786324/140‑1 (MQ=255)                                                ggAAGGATTTTTCCATGCTCAGGATCTGGAGATTTATGACCAGCACCGCCTGACCACCTGCGTACACAGCAACTGGCAGCTCAAAGCGCTGCAAAATGCGCGGCTAAAAGCACCGTTGGatat                                                                                                      >  2:126721/1‑123 (MQ=255)                                                ggAAGGATTTTTCCATGCTCAGGATCTGGAGATTTATGACCAGCACCGCCTGACCACCTGCGTACACAGCAACTGGCAGCTCAAAGCGCTGCAAAATGCGCGGCTAAAAGCACCGTTGGatat                                                                                                      <  1:126721/123‑1 (MQ=255)                                                ggAAGGATTTTTCCATGCTCAGGATCTGGAGATTTATGACCAGCACCGCCTGACCACCTGCGTACACAGCAACTGGCAGCTCAAAGCGCTGCAAAATGCGCGGCTAAAAGCACCGTTGGATATTTATCTTAAAGTGaa                                                                                       <  1:54762/138‑1 (MQ=255)                                                ggAAGGATTTTTCCATGCTCAGGATCTGGAGATTTATGACCAGCACCGCCTGACCACCTGCGTACACAGCAACTGGCAGCTCAAAGCGCTGCAAAATGCGCGGCTAAAAGCACCGTTGGATATTTATCTTAAAGTGaa                                                                                       >  2:54762/1‑138 (MQ=255)                                                ggAAGGATTTTTCCATGCTCAGGATCTGGAGATTTATGACCAGCACCGCCTGACCACCTGCGTACACAGCAACTGGCAGCTCAAAGCGCTGCAAAATGCGCGGCTAAAAGCACCGTTGGATATTTATCTTAAAGTGAACa                                                                                     >  1:691716/1‑140 (MQ=255)                                                ggAAGGATTTTTCCATGCTCAGGATCTGGAGATTTATGACCAGCACCGCCTGACCACCTGCGTACACAGCAACTGGCAGCTCAAAGCGCTGCAAAATGCGCGGCTAAAAGCACCGTTGGATATTTATCTTAAAGTGAACa                                                                                     >  2:110726/1‑140 (MQ=255)                                                                            gagaTTTATGACCAGCACCGCCTGACCACCTGCGTACACAGCAACTGGCAGCTCAAAGCGCTGCAAAATGCGCGGCTAAAAGCACCGTTGGATATTTATCTTAAAGTGAACAGTGGGATGAATCGGTTg                                                                    >  1:282873/1‑129 (MQ=255)                                                                            gagaTTTATGACCAGCACCGCCTGACCACCTGCGTACACAGCAACTGGCAGCTCAAAGCGCTGCAAAATGCGCGGCTAAAAGCACCGTTGGATATTTATCTTAAAGTGAACAGTGGGATGAATCGGTTg                                                                    <  2:282873/129‑1 (MQ=255)                                                                            gagaTTTATGACCAGCACCGCCTGACCACCTGCGTACACAGCAACTGGCAGCTCAAAGCGCTGCAAAATGCGCGGCTAAAAGCACCGTTGGATATTTATCTTAAAGTGAACAGTGGGATGAATCGGTTGGGCTTCCAGcc                                                         <  1:110726/140‑1 (MQ=255)                                                                              gaTTTATGACCAGCACCGCCTGACCACCTGCGTACACAGCAACTGGCAGCTCAAAGCGCTGCAAAATGCGCGGCTAAAAGCACCGTTGGATATTTATCTTAAAGTGAACAGTGGGATGAATCGGTTGGGCTTCCAGCCCg                                                       >  2:454982/1‑140 (MQ=255)                                                                                tttATGACCAGCACCGCCTGACCACCTGCGTACACAGCAACTGGCAGCTCAAAGCGCTGCAAAATGCGCGGCTAAAAGCACCGTTGGATATTTATCTTAAAGTGAACAGTGGGATGAATCGGTTGGGCTTCCAGCCCGAt                                                     <  2:646911/140‑1 (MQ=255)                                                                                    tGACCAGCACCGCCTGACCACCTGCGTACACAGCAACTGGCAGCTCAAAGCGCTGCAAAATGCGCGGCTAAAAGCACCGTTGGATATTTATCTTAAAGTGAACAGTGGGATGAATCGGTTGGGCTTCCAGCCCGATcgcg                                                 >  1:757247/1‑140 (MQ=255)                                                                                           cACCGCCTGACCACCTGCGTACACAGCAACTGGCAGCTCAAAGCGCTGCAAAATGCGCGGCTAAAAGCACCGTTGGATATTTATCTTAAAGTGAACAGTGGGATGAATCGGTTGGGCTTCCAGCCCGATCGCGTGCTTAc                                          <  2:94196/140‑1 (MQ=255)                                                                                               gCCTGACCACCTGCGTACACAGCAACTGGCAGCTCAAAGCGCTGCAAAATGCGCGGCTAAAAGCACCGTTGGATATTTATCTTAAAGTGAACAGTGGGATGAATCGGTTTGGCTTCCAGCCCGATCGCGTGCTTACCGTc                                      >  2:496700/1‑140 (MQ=255)                                                                                                ccTGACCACCTGCGTACACAGCAACTGGCAGCTCAAAGCGCTGCAAAATGCGCGGCTAAAAGCACCGTTGGATATTTATCTTAAAGTGAACAGTGGGATGAATCGGTTGGGCTTCCAGCCCGATCGCGTGCt                                             <  2:698006/132‑1 (MQ=255)                                                                                                ccTGACCACCTGCGTACACAGCAACTGGCAGCTCAAAGCGCTGCAAAATGCGCGGCTAAAAGCACCGTTGGATATTTATCTTAAAGTGAACAGTGGGATGAATCGGTTGGGCTTCCAGCCCGATCGCGTGCt                                             >  1:698006/1‑132 (MQ=255)                                                                                                    accaccTGCGTACACAGCAACTGGCAGCTCAAAGCGCTGCAAAATGCGCGGCTAAAAGCACCGTTGGATATTTATCTTAAAGTGAACAGTGGGATGAATCGGTTGGGCTTCCAGCCCGATCGCGTGCTTACCGTCTGgca                                 >  1:413347/1‑140 (MQ=255)                                                                                                                cacaGCAACTGGCAGCTCAAAGCGCTGCAAAATGCGCGGCTAAAAGCACCGTTGGATATTTATCTTAAAGTGAACAGTGGGATGAATCGGTTGGGCTTCCAGCCCGATCGCGTGCTTACCGTCTGGCAGCAGTTGCGGGc                     >  1:254159/1‑140 (MQ=255)                                                                                                                 acaGCAACTGGCAGCTCAAAGCGCTGCAAAATGCGCGGCTAAAAGCACCGTTGGATATTTATCTTAAAGTGAACAGTGGGATGAATCGGTTGGGCTTCCAGCCCGATCGCGTGCTTACCGTCTGGCAGCAGTTGCGGGCa                    <  2:16802/140‑1 (MQ=255)                                                                                                                   aGCAACTGGCAGCTCAAAGCGCTGCAAAATGCGCGGCTAAAAGCACCGTTGGATATTTATCTTAAAGTGAACAGTGGGATGAATCGGTTGGGCTTCCAGCCCGATCGCGTGCTTACCGTCTGGCAGCAGTTGCGGGCAAt                  >  2:493436/1‑140 (MQ=255)                                                                                                                   aGCAACTGGCAGCTCAAAGCGCTGCAAAATGCGCGGCTAAAAGCACCGTTGGATATTTATCTTAAAGTGAACAGTGGGATGAATCGGTTGGGCTTCCAGCCCGATCGCGTGCTTACCGTCTGGCAGCAGTTGCGGGCAAt                  >  1:274702/1‑140 (MQ=255)                                                                                                                   aGCAACTGGCAGCTCAAAGCGCTGCAAAATGCGCGGCTAAAAGCACCGTTGGATATTTATCTTAAAGTGAACAGTGGGATGAATCGGTTGGGCTTCCAGCCCGATCGCGTGCTTACCGTCTGGCAGCAGTTGCGGGCAAt                  >  1:709832/1‑140 (MQ=255)                                                                                                                     cAACTGGCAGCTCAAAGCGCTGCAAAATGCGCGGCTAAAAGCACCGTTGGATATTTATCTTAAAGTGAACAGTGGGATGAATCGGTTGGGCTTCCAGCCCGATCGCGTGCTTACCGTCTGGCAGCAGTTGCGGGCAATgg                >  1:674255/1‑140 (MQ=255)                                                                                                                        cTGGCAGCTCAAAGCGCTGCAAAATGCGCGGCTAAAAGCACCGTTGGATATTTATCTTAAAGTGAACAGTGGGATGAATCGGTTGGGCTTCCAGCCCGATCGCGTGCTTACCGTCTGGCAGCAGTTGCGGGCAATGGCGa             >  1:53702/1‑140 (MQ=255)                                                                                                                        cTGGCAGCTCAAAGCGCTGCAAAATGCGCGGCTAAAAGCACCGTTGGATATTTATCTTAAAGTGAACAGTGGGATGAATCGGTTGGGCTTCCAGCCCGATCGCGTGCTTACCGTCTGGCAGCAGTTGCGGGCAATGGCGa             <  2:574469/140‑1 (MQ=255)                                                                                                                           gCAGCTCAAAGCGCTGCAAAATGCGCGGCTAAAAGCACCGTTGGATATTTATCTTAAAGTGAACAGTGGGATGAATCGGTTGGGCTTCCAGCCCGATCGCGTGCTTACCGTCTGGCAGCAGTTGCGGGCAATGGCGAATg          <  2:709832/140‑1 (MQ=255)                                                                                                                               cTCAAAGCGCTGCAAAATGCGCGGCTAAAAGCACCGTTGGATATTTATCTTAAAGTGAACAGTGGGATGAATCGGTTGGGCTTCCAGCCCGATCGCGTGCTTACCGTCTGGCAGCAGTTGCGGGCAATGGCGAATGTTgg      >  1:405577/1‑140 (MQ=255)                                                                                                                                tCAAAGCGCTGCAAAATGCGCGGCTAAAAGCACCGTTGGATATTTATCTTAAAGTGAACAGTGGGATGAATCGGTTGGGCTTCCAGCCCGATCGCGTGCTTACCGTCTGGCAGCAGTTGCGGGCAATGGCGAATGTTGGc     >  2:41538/1‑140 (MQ=255)                                                                                                                                   aaGCGCTGCAAAATGCGCGGCTAAAAGCACCGTTGGATATTTATCTTAAAGTGAACAGTGGGATGAATCGGTTGGGCTTCCAGCCCGATCGCGTGCTTACCGTCTGGCAGCAGTTGCGGGCAATGGCGAATGTTGGCGaa  <  2:646646/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        GCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCATGCTCAGGATCTGGAGATTTATGACCAGCACCGCCTGACCACCTGCGTACACAGCAACTGGCAGCTCAAAGCACTGCAAAATGCGCGGCTAAAAGCACCGTTGGATATTTATCTTAAAGTGAACAGTGGGATGAATCGGTTGGGCTTCCAGCCCGATCGCGTGCTTACCGTCTGGCAGCAGTTGCGGGCAATGGCGAATGTTGGCGAA  >  NC_000913/1239068‑1239337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑