Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,365,592	C→T	P15S (CCG→TCG)	puuE →	4‑aminobutyrate aminotransferase PuuE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,365,592	0	C	T	100.0%	95.1 / NA	28	P15S (CCG→TCG)	puuE	4‑aminobutyrate aminotransferase PuuE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/13);  total (15/13)

GTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGTTTAATTTCCGATTAACCGTGAAGAGTCAAAAGGTGTGAAACATGAGCAACAATGAATTCCATCAGCGTCGTCTTTCTGCCACTCCGCGCGGGGTTGGCGTGATGTGTAACTTCTTCGCCCAGTCGGCTGAAAACGCCACGCTGAAGGATGTTGAGGGCAACGAGTACATCGATTTCGCCGCAGGCATTGCGGTGCTGAATACCGGACATCGC  >  NC_000913/1365458‑1365720                                                                                                                                       |                                                                                                                                 gTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGTTTAATTTCCGATTAACCGTGAAGAGTCAAAAGGTGTGAAACATGAGCAACAATGAATTCCATCAGCGTCGTCTTTCTGCCACTTcgcgc                                                                                                                             >  2:435631/1‑140 (MQ=255)                    gcgTATTACAGCCTGCGCGATCGTCTGGGCGTTTAATTTCCGATTAACCGTGAAGAGTCAAAAGGTGTGAAACATGAGCAACAATGAATTCCATCAGCGTCGTCTTTCTGCCACTTCGCGCGGGGTTGGCGTGATGTGTa                                                                                                          >  1:208847/1‑140 (MQ=255)                               ccTGCGCGATCGTCTGGGCGTTTAATTTCCGATTAACCGTGAAGAGTCAAAAGGTGTGAAACATGAGCAACAATGAATTCCATCAGCGTCGTCTTTCTGCCACTTCGCGCGGGGTTGGCGTGATGTGTAACTTCTTCGcc                                                                                               <  2:631636/140‑1 (MQ=255)                               ccTGCGCGATCGTCTGGGCGTTTAATTTCCGATTAACCGTGAAGAGTCAAAAGGTGTGAAACATGAGCAACAATGAATTCCATCAGCGTCGTCTTTCTGCCACTTCGCGCGGGGTTGGCGTGATGTGTAACTTCTTCGcc                                                                                               >  2:569766/1‑140 (MQ=255)                               ccTGCGCGATCGTCTGGGCGTTTAATTTCCGATTAACCGTGAAGAGTCAAAAGGTGTGAAACATGAGCAACAATGAATTCCATCAGCGTCGTCTTTCTGCCACTTCGCGCGGGGTTGGCGTGATGTGTAACTTCTTCGcc                                                                                               >  2:367907/1‑140 (MQ=255)                                   cgcgATCGTCTGGGCGTTTAATTTCCGATTAACCGTGAAGAGTCAAAAGGTGTGAAACATGAGCAACAATGAATTCCATCAGCGTCGTCTTTCTGCCACTTCGCGCGGGGTTGGCGTGATGTGTAACTTCTTCGCCCAGt                                                                                           >  1:234664/1‑140 (MQ=255)                                   cgcgATCGTCTGGGCGTTTAATTTCCGATTAACCGTGAAGAGTCAAAAGGTGTGAAACATGAGCAACAATGAATTCCATCAGCGTCGTCTTTCTGCCACTTCGCGCGGGGTTGGCGTGATGTGTAACTTCTTCGCCCAGt                                                                                           >  1:405084/1‑140 (MQ=255)                                   cgcgATCGTCTGGGCGTTTAATTTCCGATTAACCGTGAAGAGTCAAAAGGTGTGAAACATGAGCAACAATGAATTCCATCAGCGTCGTCTTTCTGCCACTTCGCGCGGGGTTGGCGTGATGTGTAACTTCTTCGCCCAGt                                                                                           >  2:428466/1‑140 (MQ=255)                                   cgcgATCGTCTGGGCGTTTAATTTCCGATTAACCGTGAAGAGTCAAAAGGTGTGAAACATGAGCAACAATGAATTCCATCAGCGTCGTCTTTCTGCCACTTCGCGCGGGGTTGGCGTGATGTGTAACTTCTTCGCCCAGt                                                                                           >  1:767550/1‑140 (MQ=255)                                               ggCGTTTAATTTCCGATTAACCGTGAAGAGTCAAAAGGTGTGAAACATGAGCAACAATGAATTCCATCAGCGTCGTCTTTCTGCCACTTCGCGCGGGGTTGGCGTGATGTGTAACTTCTTCGCCCAGTCGGCTGAAAACg                                                                               <  2:767550/140‑1 (MQ=255)                                                 cGTTTAATTTCCGATTAACCGTGAAGAGTCAAAAGGTGTGAAACATGAGCAACAATGAATTCCATCAGCGTCGTCTTTCTGCCACTTCGCGCGGGGTTGGCGTGATGTGTAACTTCTTCGCCCAGTCGGCTGAAAACGcc                                                                             <  2:208847/140‑1 (MQ=255)                                                   tttAATTTCCGATTAACCGTGAAGAGTCAAAAGGTGTGAAACATGAGCAACAATGAATTCCATCAGCGTCGTCTTTCTGCCACTTCGCGCGGGGTTGGCGTGATGTGTAACTTCTTCGCCCAGTCGGCTGAAAACGCCAc                                                                           <  1:435631/140‑1 (MQ=255)                                                    ttAATTTCCGATTAACCGTGAAGAGTCAAAAGGTGTGAAACATGAGCAACAATGAATTCCATCAGCGTCGTCTTTCTGCCACTTCGCGCGGGGTTGGCGTGATGTGTAACTTCTTCGCCCAGTCGGCTGAAAACGCCACg                                                                          <  1:669928/140‑1 (MQ=255)                                                                      tGAAGAGTCAAAAGGTGTGAAACATGAGCAACAATGAATTCCATCAGCGTCGTCTTTCTGCCACTTCGCGCGGGGTTGGCGTGATGTGTAACTTCTTCGCCCAGTCGGCTGAAAACGCCACGCTGAAGGATGTTGAGGGc                                                        <  1:741673/140‑1 (MQ=255)                                                                             tCAAAAGGTGTGAAACATGAGCAACAATGAATTCCATCAGCGTCGTCTTTCTGCCACTTCGCGCGGGGTTGGCGTGATGTGTAACTTCTTCGCCCAGTCGGCTGAAAACGCCACGCTGAAGGATGt                                                               <  2:601762/126‑1 (MQ=255)                                                                             tCAAAAGGTGTGAAACATGAGCAACAATGAATTCCATCAGCGTCGTCTTTCTGCCACTTCGCGCGGGGTTGGCGTGATGTGTAACTTCTTCGCCCAGTCGGCTGAAAACGCCACGCTGAAGGATGt                                                               >  1:601762/1‑126 (MQ=255)                                                                             tCAAAAGGTGTGAAACATGAGCAACAATGAATTCCATCAGCGTCGTCTTTCTGCCACTTCGCGCGGGGTTGGCGTGATGTGTAACTTCTTCGCCCAGTCGGCTGAAAACGCCACGCTGAAGGATGTTGAGGGCAACGAGt                                                 >  2:388149/1‑140 (MQ=255)                                                                             tCAAAAGGTGTGAAACATGAGCAACAATGAATTCCATCAGCGTCGTCTTTCTGCCACTTCGCGCGGGGTTGGCGTGATGTGTAACTTCTTCGCCCAGTCGGCTGAAAACGCCACGCTGAAGGATGTTGAGGGCAACGAGt                                                 >  2:516905/1‑140 (MQ=255)                                                                                       tgAAACATGAGCAACAATGAATTCCATCAGCGTCGTCTTTCTGCCACTTCGCGCGGGGTTGGCGTGATGTGTAACTTCTTCGCCCAGTCGGCTGAAAACGCCACGCTGAAGGATGTTGAGGGCAACGAGTACATCGAttt                                       >  2:544111/1‑140 (MQ=255)                                                                                                aGCAACAATGAATTCCATCAGCGTCGTCTTTCTGCCACTTCGCGCGGGGTTGGCGTGATGTGTAACTTCTTCGCCCAGTCGGCTGAAAACGCCACGCTGAAGGATGTTGAGGGCAACGAGTACATCGATTTCGCCGCAgg                              >  1:697016/1‑140 (MQ=255)                                                                                                     caaTGAATTCCATCAGCGTCGTCTTTCTGCCACTTCGCGCGGGGTTGGCGTGATGTGTAACTTCTTCGCCCAGTCGGCTGAAAACGCCACGCTGAAGGATGTTGAGGGCAACGAGTACATCGATTTCGCCGCAGGCATTg                         <  1:618184/140‑1 (MQ=255)                                                                                                       aTGAATTCCATCAGCGTCGTCTTTCTGCCACTTCGCGCGGGGTTGGCGTGATGTGTAACTTCTTCGCCCAGTCGGCTGAAAACGCCACGCTGAAGGATGTTGAGGGCAACGAGTACATCGATTTCGCCGCAGGCATTGCg                       <  1:428466/140‑1 (MQ=255)                                                                                                                    gCGTCGTCTTTCTGCCACTTCGCGCGGGGTTGGCGTGATGTGTAACTTCTTCGCCCAGTCGGCTGAAAACGCCACGCTGAAGGATGTTGAGGGCAACGAGTACATCGATTTCGCCGCAGGCATTg                         <  2:70554/125‑1 (MQ=255)                                                                                                                    gCGTCGTCTTTCTGCCACTTCGCGCGGGGTTGGCGTGATGTGTAACTTCTTCGCCCAGTCGGCTGAAAACGCCACGCTGAAGGATGTTGAGGGCAACGAGTACATCGATTTCGCCGCAGGCATTg                         >  1:70554/1‑125 (MQ=255)                                                                                                                      gtcgtcTTTCTGCCACTTCGCGCGGGGTTGGCGTGATGTGTAACTTCTTCGCCCAGTCGGCTGAAAACGCCACGCTGAAGGATGTTGAGGGCAACGAGTACATCGATTTCGCCGCAGGCATTGCGGTGCTGAATACCGGa        <  2:405084/140‑1 (MQ=255)                                                                                                                         gtcTTTCTGCCACTTCGCGCGGGGTTGGCGTGATGTGTAACTTCTTCGCCCAGTCGGCTGAAAACGCCACGCTGAAGGATGTTGAGGGCAACGAGTACATCGATTTCGCCGCAGGCATTGCGGTGCTGAATACCGGACAt     >  2:230790/1‑140 (MQ=255)                                                                                                                         gtcTTTCTGCCACTTCGCGCGGGGTTGGCGTGATGTGTAACTTCTTCGCCCAGTCGGCTGAAAACGCCACGCTGAAGGATGTTGAGGGCAACGAGTACATCGATTTCGCCGCAGGCATTGCGGTGCTGAATACCGGACAt     >  1:807850/1‑140 (MQ=255)                                                                                                                           cTTTCTGCCACTTCGCGCGGGGTTGGCGTGATGTGTAACTTCTTCGCCCAGTCGGCTGAAAACGCCACGCTGAAGGATGTTGAGGGCAACGAGTACATCGATTTCGCCGCAGGCATTGCGGTGCTGAATACCGGACATCg   <  1:768975/140‑1 (MQ=255)                                                                                                                            tttCTGCCACTTCGCGCGGGGTTGGCGTGATGTGTAACTTCTTCGCCCAGTCGGCTGAAAACGCCACGCTGAAGGATGTTGAGGGCAACGAGTACATCGATTTCGCCGCAGGCATTGCGGTGCTGAATACCGGACATCGc  <  1:516905/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                 GTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGTTTAATTTCCGATTAACCGTGAAGAGTCAAAAGGTGTGAAACATGAGCAACAATGAATTCCATCAGCGTCGTCTTTCTGCCACTCCGCGCGGGGTTGGCGTGATGTGTAACTTCTTCGCCCAGTCGGCTGAAAACGCCACGCTGAAGGATGTTGAGGGCAACGAGTACATCGATTTCGCCGCAGGCATTGCGGTGCTGAATACCGGACATCGC  >  NC_000913/1365458‑1365720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑