Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,476,379	A→G	G412G (GGA→GGG)	ydbD →	DUF2773 domain‑containing protein YdbD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,476,379	0	A	G	100.0%	155.8 / NA	44	G412G (GGA→GGG)	ydbD	DUF2773 domain‑containing protein YdbD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/22);  total (22/22)

TCATGACTCTGGCGAAAAGAGATTGGGATATGATCATTCAGGAGCTTTATAAAGATGCGTGGTTATTGCCTGAATCCATTATTGATGGCTATATCCGCAGTGATGATTCCTCTATTCGTCAGGTCGGTGCTGGAGGACAACTCACCTACAATCAGGCAATGCAGTTGGCGAACGATTCATCAAACAATGTTGTCACAAGCTTAGCGTTCAAGCTGGCAGAGATGAAACACCATGGTCAATTGTTGCGGATGACGCCACAAGAGAGT  >  NC_000913/1476243‑1476508                                                                                                                                         |                                                                                                                                  tCATGACTCTGGCGAAAAGAGATTGGGATATGATCATTCAGGAGCTTTATAAAGATGCGTGGTTATTGCCTGAATCCATTATTGATGGCTATATCCGCAGTGATGATTCCTCTATTCGTCAGGTCGGTGCTGGAGGGCaa                                                                                                                                <  2:709517/140‑1 (MQ=255)  cATGACTCTGGCGAAAAGAGATTGGGATATGATCATTCAGGAGCTTTATAAAGATGCGTGGTTATTGCCTGAATCCATTATTGATGGCTATATCCGCAGTGATGATTCCTCTATTCGTCAGGTCGGTGCTGGAGGGCaa                                                                                                                                >  2:394881/1‑139 (MQ=255)  cATGACTCTGGCGAAAAGAGATTGGGATATGATCATTCAGGAGCTTTATAAAGATGCGTGGTTATTGCCTGAATCCATTATTGATGGCTATATCCGCAGTGATGATTCCTCTATTCGTCAGGTCGGTGCTGGAGGGCaa                                                                                                                                <  1:394881/139‑1 (MQ=255)  cATGACTCTGGCGAAAAGAGATTGGGATATGATCATTCAGGAGCTTTATAAAGATGCGTGGTTATTGCCTGAATCCATTATTGATGGCTATATCCGCAGTGATGATTCCTCTATTCGTCAGGTCGGTGCTGGAGGGCAAc                                                                                                                               <  1:291061/140‑1 (MQ=255)   aTGACTCTGGCGAAAAGAGATTGGGATATGATCATTCAGGAGCTTTATAAAGATGCGTGGTTATTGCCTGAATCCATTATTGATGGCTATATCCGTAGTGAGGATTCCGCTATTCGTCTGGGCGGTGCTGGAGGGCAACt                                                                                                                              <  2:215330/140‑1 (MQ=255)          tGGCGAAAAGAGATTGGGATATGATCATTCAGGAGCTTTATAAAGATGCGTGGTTATTGCCTGAATCCATTATTGATGGCTATATCCGCAGTGATGATTCCTCTATTCGTCAGGTCGGTGCTGGAGGGCAACTCACCTAc                                                                                                                       >  1:806497/1‑140 (MQ=255)                 aaGAGATTGGGATATGATCATTCAGGAGCTTTATAAAGATGCGTGGTTATTGCCTGAATCCATTATTGATGGCTATATCCGCAGTGATGATTCCTCTATTCGTCAGGTCGGTGCTGGAGGGCAACTCACCTACAATCAgg                                                                                                                <  2:806497/140‑1 (MQ=255)                      aTTGGGATATGATCATTCAGGAGCTTTATAAAGATGCGTGGTTATTGCCTGAATCCATTATTGATGGCTATATCCGCAGTGATGATTCCTCTATTCGTCAGGTCGGTGCTGGAGGGCAACTCACCTACAATCAGGCAATg                                                                                                           <  2:497963/140‑1 (MQ=255)                                 aTCATTCAGGAGCTTTATAAAGATGCGTGGTTATTGCCTGAATCCATTATTGATGGCTATATCCGCAGTGATGATTCCTCTATTTGTCAGGTCGGTGCTGGAGGGCAACTCACCTACAATCAGGCAATGCAGTTGGCGaa                                                                                                <  1:265997/140‑1 (MQ=255)                                     ttCAGGAGCTTTATAAAGATGCGTGGTTATTGCCTGAATCCATTATTGATGGCTATATCCGCAGTGATGATTCCTCTATTCGTCAGGTCGGTGCTGGAGGGCAACTCACCTACAATCAGGCAATGCAGTTGGCGAACGAt                                                                                            <  2:428335/140‑1 (MQ=255)                                     ttCAGGAGCTTTATAAAGATGCGTGGTTATTGCCTGAATCCATTATTGATGGCTATATCCGCAGTGATGATTCCTCTATTCGTCAGGTCGGTGCTGGAGGGCAACTCACCTACAATCAGGCAATGCAGTTGGCGAACGAt                                                                                            <  1:103175/140‑1 (MQ=255)                                      tCAGGAGCTTTATAAAGATGCGTGGTTATTGCCTGAATCCATTATTGATGGCTATATCCGCAGTGATGATTCCTCTATTCGTCAGGTCGGTGCTGGAGGGCAACTCACCTACAATCAGGCAATGCAGTTGGCGAACGAtt                                                                                           >  2:198962/1‑140 (MQ=255)                                        aGGAGCTTTATAAAGATGCGTGGTTATTGCCTGAATCCATTATTGATGGCTATATCCGCAGTGATGATTCCTCTATTCGTCAGGTCGGTGCTGGAGGGCAACTCACCTACAATCAGGCAATGCAGTTGGCGAACGATtca                                                                                         <  1:198962/140‑1 (MQ=255)                                          gAGCTTTATAGAGATGCGTGGTTATTGCCTGAATCCATTATTGATGGCTATATCCGCAGTGATGATTCCTCTATTCGTCAGGTCGGTGCTGGATTGCAACTCACCTACAATCAGGCAATGCAGTTGGCGAACGATtcata                                                                                       >  2:445376/1‑139 (MQ=255)                                          gAGCTTTATAAAGATGCGTGGTTATTGCCTGAATCCATTATTGATGGCTATATCCGCAGTGATGATTCCTCTATTCGTCAGGTCGGTGCTGGAGGGCAACTCACCTACAATCAGGCAATGCAGTTGGCGAACGATtcatc                                                                                       >  1:365907/1‑140 (MQ=255)                                               ttATAAAGATGCGTGGTTATTGCCTGAATCCATTATTGATGGCTATATCCGCAGTGATGATTCCTCTATTCGTCAGGTCGGTGCTGGAGGGCAACTCACCTACAATCAGGCAATGCAGTTGGCGAACGATTCATCAAACa                                                                                  <  2:658361/140‑1 (MQ=255)                                               ttATAAAGATGCGTGGTTATTGCCTGAATCCATTATTGATGGCTATATCCGCAGTGATGATTCCTCTATTCGTCAGGTCGGTGCTGGAGGGCAACTCACCTACAATCAGGCAATGCAGTTGGCGAACGATTCATCAAACa                                                                                  <  2:752178/140‑1 (MQ=255)                                               ttATAAAGATGCGTGGTTATTGCCTGAATCCATTATTGATGGCTATATCCGCAGTGATGATTCCTCTATTCGTCAGGTCGGTGCTGGAGGGCAACTCACCTACAATCAGGCAATGCAGTTGGCGAACGATTCATCAAACa                                                                                  >  1:644289/1‑140 (MQ=255)                                                tataAAGATGCGTGGTTATTGCCTGAATCCATTATTGATGGCTATATCCGCAGTGATGATTCCTCTATTCGTCAGGTCGGTGCTGGAGGGCAACTCACCTACAATCAGGCAATGCAGTTGGCGAACGATTCATCAAACaa                                                                                 >  2:457420/1‑140 (MQ=255)                                                         gCGTGGTTATTGCCTGAATCCATTATTGATGGCTATATCCGCAGTGATGATTCCTCTATTCGTCAGGTCGGTGCTGGAGGGCAACTCACCTACAATCAGGCAATGCAGTTGGCGAACGATTCATCAAACAATGTTGTcac                                                                        <  1:502246/140‑1 (MQ=255)                                                          cGTGGTTATTGCCTGAATCCATTATTGATGGCTATATCCGCAGTGATGATTCCTCTATTCGTCAGGTCGGTGCTGGAGGGCAACTCACCTACAATCAGGCAATGCAGTTGGCGAACGATTCATCAAACAATGTTGTcaca                                                                       >  1:665142/1‑140 (MQ=255)                                                          cGTGGTTATTGCCTGAATCCATTATTGATGGCTATATCCGCAGTGATGATTCCTCTATTCGTCAGGTCGGTGCTGGAGGGCAACTCACCTACAATCAGGCAATGCAGTTGGCGAACGATTCATCAAACAATGTTGTcaca                                                                       >  2:229255/1‑140 (MQ=255)                                                                       tGAATCCATTATTGATGGCTATATCCGCAGTGATGATTCCTCTATTCGTCAGGTCGGTGCTGGAGGGCAACTCACCTACAATCAGGCAATGCAGTTGGCGAACGATTCATCAAACAATGTTGTCACAAGCTTAGCGTTCa                                                          >  2:333559/1‑140 (MQ=255)                                                                        gAATCCATTATTGATGGCTATATCCGCAGTGATGATTCCTCTATTCGTCAGGTCGGTGCTGGAGGGCAACTCACCTACAATCAGGCAATGCAGTTGGCGAACGATTCATCAAACAATGTTGTCACAAGCTTAGCGTTCaa                                                         >  2:540192/1‑140 (MQ=255)                                                                         aaTCCATTATTGATGGCTATATCCGCAGTGATGATTCCTCTATTCGTCAGGTCGGTGCTGGAGGGCAACTCACCTACAATCAGGCAATGCAGTTGGCGAACGATTCATCAAACAATGTTGTCACAAGCTTAGCGTTCAAg                                                        >  1:264930/1‑140 (MQ=255)                                                                           tCCATTATTGATGGCTATATCCGCAGTGATGATTCCTCTATTCGTCAGGTCGGTGCTGGAGGGCAACTCACCTACAATCAGGCAATGCAGTTGGCGAACGATTCATCAAACAATGTTGTCACAAGCTTAGCGTTCAAGCt                                                      >  1:430244/1‑140 (MQ=255)                                                                                         cTATATCCGCAGTGATGATTCCTCTATTCGTCAGGTCGGTGCTGGAGGGCAACTCACCTACAATCAGGCAATGCAGTTGGCGAACGATTCATCAAACAATGTTGTCACAAGCTTAGCGTTCAAGCTGGCAGAGATGAAac                                        >  2:491616/1‑140 (MQ=255)                                                                                             atCCGCAGTGATGATTCCTCTATTCGTCAGGTCGGTGCTGGAGGGCAACTCACCTACAATCAGGCAATGCAGTTGGCGAACGATTCATCAAACAATGTTGTCACAAGCTTAGCGTTCAAGCTGGCAGAGATGAAACACCa                                    <  2:285736/140‑1 (MQ=255)                                                                                                  cAGTGATGATTCCTCTATTCGTCAGGTCGGTGCTGGAGGGCAACTCACCTACAATCAGGCAATGCAGTTGGCGAACGATTCATCAAACAATGTTGTCACAAGCTTAGCGTTCAAGCTGGCAGAGATGAAACACCATGGTc                               <  2:644289/140‑1 (MQ=255)                                                                                                  cAGTGATGATTCCTCTATTCGTCAGGTCGGTGCTGGAGGGCAACTCACCTACAATCAGGCAATGCAGTTGGCGAACGATTCATCAAACAATGTTGTCACAAGCTTAGCGTTCAAGCTGGCAGAGATGAAACACCATGGTc                               >  1:393573/1‑140 (MQ=255)                                                                                                        tgatTCCTCTATTCGTCAGGTCGGTGCTGGAGGGCAACTCACCTACAATCAGGCAATGCAGTTGGCGAACGATTCATCAAACAATGTTGTCACAAGCTTAGCGTTCAAGCTGGCAGAGATGAAACACCATGGTCAAttgt                         >  1:267925/1‑140 (MQ=255)                                                                                                               tctATTCGTCAGGTCGGTGCTGGAGGGCAACTCACCTACAATCAGGCAATGCAGTTGGCGAACGATTCATCAAACAATGTTGTCACAAGCTTAGCGTTCAAGCTGGCAGAGATGAAACACCATGGTCAATTGTTGCGGAt                  <  2:665142/140‑1 (MQ=255)                                                                                                               tctATTCGTCAGGTCGGTGCTGGAGGGCAACTCACCTACAATCAGGCAATGCAGTTGGCGAACGATTCATCAAACAATGTTGTCACAAGCTTAGCGTTCAAGCTGGCAGAGATGAAACACCATGGTCAATTGTTGCGGAt                  >  1:352063/1‑140 (MQ=255)                                                                                                               tctATTCGTCAGGTCGGTGCTGGAGGGCAACTCACCTACAATCAGGCAATGCAGTTGGCGAACGATTCATCAAACAATGTTGTCACAAGCTTAGCGTTCAAGCTGGCAGAGATGAAACACCATGGTCAATTGTTGCGGAt                  <  1:457420/140‑1 (MQ=255)                                                                                                                ctATTCGTCAGGTCGGTGCTGGAGGGCAACTCACCTACAATCAGGCAATGCAGTTGGCGAACGATTCATCAAACAATGTTGTCACAAGCTTAGCGTTCAAGCTGGCAGAGATGAAACACCATGGTCAATTGTTGCGGATg                 >  1:175769/1‑140 (MQ=255)                                                                                                                 tATTCGTCAGGTCGGTGCTGGAGGGCAACTCACCTACAATCAGGCAATGCAGTTGGCGAACGATTCATCAAACAATGTTGTCACAAGCTTAGCGTTCAAGCTGGCAGAGATGAAACACCATGGTCAATTGTTGCGGATGa                <  2:267925/140‑1 (MQ=255)                                                                                                                   ttCGTCAGGTCGGTGCTGGAGGGCAACTCACCTACAATCAGGCAATGCAGTTGGCGAACGATTCATCAAACAATGTTGTCACAAGCTTAGCGTTCAAGCTGGCAGAGATGAAACACCATGGTCAATTGTTGCGGAt                  <  1:75806/136‑1 (MQ=255)                                                                                                                   ttCGTCAGGTCGGTGCTGGAGGGCAACTCACCTACAATCAGGCAATGCAGTTGGCGAACGATTCATCAAACAATGTTGTCACAAGCTTAGCGTTCAAGCTGGCAGAGATGAAACACCATGGTCAATTGTTGCGGAt                  >  2:75806/1‑136 (MQ=255)                                                                                                                   ttCGTCAGGTCGGTGCTGGAGGGCAACTCACCTACAATCAGGCAATGCAGTTGGCGAACGATTCATCAAACAATGTTGTCACAAGCTTAGCGTTCAAGCTGGCAGAGATGAAACACCATGGTCAATTGTTGCGGATGACg              <  2:393573/140‑1 (MQ=255)                                                                                                                      gTCAGGTCGGTGCTGGAGGGCAACTCACCTACAATCAGGCAATGCAGTTGGCGAACGATTCATCAAACAATGTTGTCACAAGCTTAGCGTTCAAGCTGGCAGAGATGAAACACCATGGTCAATTGTTGCGGATGACg              <  2:343119/137‑1 (MQ=255)                                                                                                                      gTCAGGTCGGTGCTGGAGGGCAACTCACCTACAATCAGGCAATGCAGTTGGCGAACGATTCATCAAACAATGTTGTCACAAGCTTAGCGTTCAAGCTGGCAGAGATGAAACACCATGGTCAATTGTTGCGGATGACg              >  1:343119/1‑137 (MQ=255)                                                                                                                      gTCAGGTCGGTGCTGGAGGGCAACTCACCTACAATCAGGCAATGCAGTTGGCGAACGATTCATCAAACAATGTTGTCACAAGCTTAGCGTTCAAGCTGGCAGAGATGAAACACCATGGTCAATTGTTGCGGATGACGCca           >  2:393136/1‑140 (MQ=255)                                                                                                                        cAGGTCGGTGCTGGAGGGCAACTCACCTACAATCAGGCAATGCAGTTGGCGAACGATTCATCAAACAATGTTGTCACAAGCTTAGCGTTCAAGCTGGCAGAGATGAAACACCATGGTCAATTGTTGCGGATGACGCcaca         >  1:232440/1‑140 (MQ=255)                                                                                                                           gTCGGTGCTGGAGGGCAACTCACCTACAATCAGGCAATGCAGTTGGCGAACGATTCATCAAACAATGTTGTCACAAGCTTAGCGTTCAAGCTGGCAGAGATGAAACACCATGGTCAATTGTTGCGGATGACGCCACAaga      >  1:52054/1‑140 (MQ=255)                                                                                                                            tCGGTGCTGGAGGGCAACTCACCTACAATCAGGCAATGCAGTTGGCGAACGATTCATCAAACAATGTTGTCACAAGCTTAGCGTTCAAGCTGGCAGAGATGAAACACCATGGTCAATTGTTGCGGATGACGCCACAagag     <  1:393136/140‑1 (MQ=255)                                                                                                                               gTGCTGGAGGGCAACTCACCTACAATCAGGCAATGCAGTTGGCGAACGATTCATCAAACAATGTTGTCACAAGCTTAGCGTTCAAGCTGGCAGAGATGAAACACCATGGTCAATTGTTGCGGATGACGCCACAAGAGAGt  <  2:175769/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                  TCATGACTCTGGCGAAAAGAGATTGGGATATGATCATTCAGGAGCTTTATAAAGATGCGTGGTTATTGCCTGAATCCATTATTGATGGCTATATCCGCAGTGATGATTCCTCTATTCGTCAGGTCGGTGCTGGAGGACAACTCACCTACAATCAGGCAATGCAGTTGGCGAACGATTCATCAAACAATGTTGTCACAAGCTTAGCGTTCAAGCTGGCAGAGATGAAACACCATGGTCAATTGTTGCGGATGACGCCACAAGAGAGT  >  NC_000913/1476243‑1476508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑