Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,478,403	A→G	T60A (ACG→GCG)	ynbB →	putative CDP‑diglyceride synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,478,403	0	A	G	100.0%	124.1 / NA	36	T60A (ACG→GCG)	ynbB	putative CDP‑diglyceride synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/21);  total (15/21)

TAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGC  >  NC_000913/1478281‑1478538                                                                                                                           |                                                                                                                                        tAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGGCGTTGACGTTTTTTgcg                                                                                                                        <  2:50985/140‑1 (MQ=255)        ttttCTGCTATGGCGGTTACCGGAGAGAAAAAGGGGGGGGGGTAAATTTCGTATTCGTACCTGGTGGGGGATCGTCATTTTTTTTTTTATTGTGATTTTAGGTCCACGCTGGGTGGGGGTGACGTTTTTTTTGCTGGTaa                                                                                                                 >  2:713108/1‑140 (MQ=255)                            cGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGGCGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGaa                                                                                             <  2:308368/140‑1 (MQ=255)                            cGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGGCGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGaa                                                                                             <  2:206931/140‑1 (MQ=255)                                gagaAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGGCGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATAtt                                                                                         <  1:270732/140‑1 (MQ=255)                                 agaAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGGCGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTg                                                                                        >  1:448895/1‑140 (MQ=255)                                  gaAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGGCGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGt                                                                                       <  1:745170/140‑1 (MQ=255)                                  gaAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGGCGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGt                                                                                       <  2:775115/140‑1 (MQ=255)                                             gAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGGCGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATc                                                                            >  2:740890/1‑140 (MQ=255)                                                 ttACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGGCGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTa                                                                        <  1:391369/140‑1 (MQ=255)                                                        aCGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGGCGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTc                                                                 <  1:740890/140‑1 (MQ=255)                                                         cGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGGCGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTcc                                                                >  2:200956/1‑140 (MQ=255)                                                          gTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGGCGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCcg                                                               >  2:539826/1‑140 (MQ=255)                                                          gTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGGCGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCcg                                                               >  1:259345/1‑140 (MQ=255)                                                            aTTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGGCGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCcgcg                                                             <  1:602605/140‑1 (MQ=255)                                                            aTTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGGCGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCcgcg                                                             <  2:263492/140‑1 (MQ=255)                                                             ttCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGGCGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGt                                                            <  2:523066/140‑1 (MQ=255)                                                              tCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGGCGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGtt                                                           >  2:127148/1‑140 (MQ=255)                                                               cGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGGCGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTg                                                          >  2:454735/1‑140 (MQ=255)                                                                gTACCTGGTGGGGCATCGTTATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGGCGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTgg                                                         <  2:666785/140‑1 (MQ=255)                                                                gTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGGCGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTgg                                                         <  1:539826/140‑1 (MQ=255)                                                                 tACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGGCGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGt                                                        >  1:204133/1‑140 (MQ=255)                                                                     tggtggGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGGCGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTtata                                                    >  1:147187/1‑140 (MQ=255)                                                                        tggGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGGCGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTg                                                 >  1:570145/1‑140 (MQ=255)                                                                        tggGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGGCGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTg                                                 <  2:276551/140‑1 (MQ=255)                                                                        tggGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGGCGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTg                                                 >  1:807807/1‑140 (MQ=255)                                                                          gggCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGGCGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTggg                                               >  2:486858/1‑140 (MQ=255)                                                                             cATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGGCGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTa                                            <  1:633078/140‑1 (MQ=255)                                                                             cATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGGCGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTa                                            <  1:272515/140‑1 (MQ=255)                                                                                  tCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGGCGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCct                                       >  1:163916/1‑140 (MQ=255)                                                                                     tttGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGGCGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCtt                                    >  1:501825/1‑140 (MQ=255)                                                                                           tttCAATGGTGATTTCAGGTCCACGCTGGATGGCGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTAt                              <  2:163916/140‑1 (MQ=255)                                                                                            ttCAATGGTGATTTCAGGTCCACGCTGGATGGCGTTGACGTTTTTTGCGTTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTGCGCGTTGGTTATATTGGGGTATTCCTCTTAACTAtt                             >  2:599356/1‑140 (MQ=255)                                                                                                           ggttCCACGCTGGAGGCGGTTGACGTTTTTGGCGCTGATAAGTTTCTTGGCATGAAAAGATATTTGTCGGCTAATTTCTTTCCATTTTCCGCTTTGGTTTTATT‑GGGTATTCCTCTTAACTATTTGCTTATCGGTTTTaa             <  2:317936/137‑1 (MQ=255)                                                                                                                    cTGGATGGCGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTg     <  1:272254/140‑1 (MQ=255)                                                                                                                    cTGGATGGCGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTg     <  1:384045/140‑1 (MQ=255)                                                                                                                      ggATGCCGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAACATTGTACGCTTATATCTGTACGTTTTCCGCGTTGGTTATATTGGGATAGTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAg   <  1:599356/140‑1 (MQ=255)                                                                                                                       gATGGCGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGc  <  1:655967/140‑1 (MQ=255)                                                                                                                       gATGGCGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGc  <  1:183345/140‑1 (MQ=255)                                                                                                                           |                                                                                                                                        TAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGC  >  NC_000913/1478281‑1478538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑