Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,487,156	A→G	intergenic (+193/‑79)	hrpA → / → ydcF	ATP‑dependent RNA helicase HrpA/DUF218 domain‑containing protein YdcF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,487,156	0	A	G	100.0%	49.2 / NA	15	intergenic (+193/‑79)	hrpA/ydcF	ATP‑dependent RNA helicase HrpA/DUF218 domain‑containing protein YdcF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/15);  total (0/15)

ATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAACCATGAACATTACCCCGTTTCCGACGCTTTCGCCGGCAACTATAGATGCCATAAATG  >  NC_000913/1487021‑1487289                                                                                                                                        |                                                                                                                                      attaATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATGCGAt                                                                                                                                   <  1:604587/140‑1 (MQ=255)              cGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATGCGATTTCCTCCTTTCag                                                                                                                      <  1:671256/140‑1 (MQ=255)                    gggTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATGCGATTTCCTCCTTTCAGAGTGCt                                                                                                                <  1:280511/140‑1 (MQ=255)                    gggTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATGCGATTTCCTCCTTTCAGAGTGCt                                                                                                                <  1:9329/140‑1 (MQ=255)                       tAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATGCGATTTCCTCCTTTCAGAGTGCTCCg                                                                                                             <  2:199182/140‑1 (MQ=255)                          aaaTTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATGCGATTTCCTCCTCTCAGAGTGCTCCGCtt                                                                                                          <  2:793471/140‑1 (MQ=255)                           aaTTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATGCGATTTCCTCCTTTCAGAGTGCTCCGCTtc                                                                                                         <  2:731499/140‑1 (MQ=255)                            aTTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATGCGATTTCCTCCTTTCAGAGTGCTCCGCTtct                                                                                                        <  1:375784/140‑1 (MQ=255)                              tCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATGCGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCa                                                                                                      <  2:797/140‑1 (MQ=255)                                    aTTCAGAAAAATAAAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATGCGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACtatta                                                                                                <  2:262638/140‑1 (MQ=255)                                     ttCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAAGAAAAGCGAATTTCTCCTTTTTGAGTGCTCCCCCTCTCCCtattat                                                                                               >  1:535808/1‑140 (MQ=255)                                           aaaaTACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATGCGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACg                                                                                         <  1:53930/140‑1 (MQ=255)                                                          gctgcAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATGCGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAggg                                                                          <  1:479405/140‑1 (MQ=255)                                                                                     aGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGCTAAAGACAAGCGATTTTCTCCTTTTCTAAGGCGCCGCCTCTCCCTATTATTTTTCGCCGAATTCTTTAAAGAAAGAAAAAGAGGGAACAAGaaaaata                                               >  2:583014/1‑135 (MQ=255)                                                                                                              gCTTGCGGAGTGGCGGTTAAGGACATGCGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAACCATGAACATTACCCCGTTTCCGACGCTTTCGCCGGc                      <  1:29696/140‑1 (MQ=255)                                                                                                                                aaGGACATGCGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAACCATGAACATTACCCCGTTTCCGACGCTTTCGCCGGCAACTATAGATGCCATaaa    <  1:23764/140‑1 (MQ=255)                                                                                                                                  ggACATGCGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAACCATGAACATTACCCCGTTTCCGACGCTTTCGCCGGCAACTATAGATGCCATAAATg  <  2:535808/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                      ATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAACCATGAACATTACCCCGTTTCCGACGCTTTCGCCGGCAACTATAGATGCCATAAATG  >  NC_000913/1487021‑1487289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑