Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,555,425	T→C	D82G (GAC→GGC)	maeA ←	malate dehydrogenase, NAD‑requiring

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,555,425	0	T	C	100.0%	162.3 / NA	46	D82G (GAC→GGC)	maeA	malate dehydrogenase, NAD‑requiring
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/17);  total (29/17)

AGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGTTAGTGTCCTGGATGTTACGCAGGTAGATGTGTTTGTCGATTTCGGTTTTGAATCCCTGATACTGGATCCATGCTCGTTCCGCTTGTTCTTCGATGGTTTCGACCACTTCCGGCAGTAACCCCAGCAGGTTGAAGTTACGGCG  >  NC_000913/1555288‑1555561                                                                                                                                          |                                                                                                                                         agaTAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGTTAGTGccc                                                                                                                                        >  1:555490/1‑140 (MQ=255)  gaTAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGTTAGTGCCCt                                                                                                                                       <  2:202972/140‑1 (MQ=255)    tAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGTTAGTGCCCTgg                                                                                                                                     >  1:366524/1‑140 (MQ=255)           gccgcGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGTTAGTGCCCTGGATGTTAc                                                                                                                              <  1:802639/140‑1 (MQ=255)             cgcgTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGTTAGTGccc                                                                                                                                        >  2:37906/1‑128 (MQ=255)             cgcgTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGGTTCGTTAGTGCCCTGGATGTTACGc                                                                                                                            >  1:744614/1‑140 (MQ=255)               cgTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGTTAGTGCCCTGGATGTTACGCAg                                                                                                                          >  1:728714/1‑140 (MQ=255)                                ctcAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATTTCATCAAGATGATTGTTTACCAGACGGTAGAAGGGGGGTTCGTTAGTGCCCTGGATGTTAAGCAGGTAGGTGTGTTTGTCGa                                                                                                         >  2:808409/1‑140 (MQ=255)                                  cAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGTTAGTGCCCTGGATGTTACGCAGGTAGATGTGTTTGTCGAtt                                                                                                       >  1:155110/1‑140 (MQ=255)                                        aaCGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGTTAGTGCCCTGGATGTTACGCAGGTAGATGTGTTTGTCGATTTCGGtt                                                                                                 >  1:375575/1‑140 (MQ=255)                                                  ggCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGTTAGTGCCCTGGATGTTACGCAGGTAGATGTGTTTGTCGATTTCGGTTTTGAATCCCt                                                                                       <  1:59285/140‑1 (MQ=255)                                                       cgcCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGTTAGTGCCCTGGATGTTACGCAGGTAGATGTGTTTGTCGATTTCGGTTTTGAATccc                                                                                        <  2:719322/134‑1 (MQ=255)                                                       cgcCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGTTAGTGCCCTGGATGTTACGCAGGTAGATGTGTTTGTCGATTTCGGTTTTGAATccc                                                                                        >  1:719322/1‑134 (MQ=255)                                                       cgcCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGTTAGTGCCCTGGATGTTACGCAGGTAGATGTGTTTGTCGATTTCGGTTTTGAATCCCTGATAc                                                                                  <  1:10667/140‑1 (MQ=255)                                                             cGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGTTAGTGCCCTGGATGTTACGCAGGTAGATGTGTTTGTCGATTTCGGTTTTGAATCCCTGATACTGGATc                                                                            >  2:798679/1‑140 (MQ=255)                                                                 tGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGTTAGTGCCCTGGATGTTACGCAGGTAGATGTGTTTGTCGATTTCGGTTTTGAATCCCTGATa                                                                                   >  2:770615/1‑129 (MQ=255)                                                                 tGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGTTAGTGCCCTGGATGTTACGCAGGTAGATGTGTTTGTCGATTTCGGTTTTGAATCCCTGATa                                                                                   <  1:770615/129‑1 (MQ=255)                                                                        taaataaCAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGTTAGTGCCCTGGATGTTACGCAGGTAGATGTGTTTGCCGATTTCGGTTTTGAATCCCTGATACTGGATCCATGCTCGTTc                                                                 <  2:744614/140‑1 (MQ=255)                                                                          aataaCAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGTTAGTGCCCTGGATGTTACGCAGGTAGATGTGTTTGTCGATTTCGGTTTTGAATCCCTGATACTGGATCCATGCTCGTTCCg                                                               >  1:390505/1‑140 (MQ=255)                                                                           ataaCAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGTTAGTGCCCTGGATGTTACGCAGGTAGATGTGTTTGTCGATTTCGGTTTTGAATCCCTGATACTGGATCCATGCTCGTTCCGc                                                              <  1:808409/140‑1 (MQ=255)                                                                              aCAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGTTAGTGCCCTGGATGTTACGCAGGTAGATGTGTTTGTCGATTTCGGTTTTGAATCCCTGATACTGGATCCATGCTCGTTCCGCTTg                                                           >  2:95912/1‑140 (MQ=255)                                                                               cAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGTTAGTGCCCTGGATGTTACGCAGGTAGATGTGTTTGTCGATTTCGGTTTTGAATCCCTGATACTGGATCCATGCTCGTTCCGCTTGt                                                          >  2:707752/1‑140 (MQ=255)                                                                                 ggCATCATCTCAGCATGATGATTGTTGACCAGACGGTAGAAGAGGGTTTCGTTAGTGCCCTGGATGATACGCAGGTAGATGTGTTTGTCGATTTGGGTTTTGAATCCCTGATACTGGATCCATGCTCGTTCCGCTGGttc                                                        <  2:727626/140‑1 (MQ=255)                                                                                    atcatcTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGTTAGTGCCCTGGATGTTACGCAGGTAGATGTGTTTGTCGATTTCGGTTTTGAATCCCTGATACTGGATCCATGCTCGTTCCGCTTGttcttc                                                     >  1:446057/1‑140 (MQ=255)                                                                                     tcatctcatcAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGTTAGTGCCCTGGATGTTACGCAGGTAGATGTGTTTGTCGATTTCGGTTTTGAATCCCTGATACTGGATCCATGCTCGTTCCGCTTGTTCTTCg                                                    >  2:653216/1‑140 (MQ=255)                                                                                     tcatctcatcAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGTTAGTGCCCTGGATGTTACGCAGGTAGATGTGTTTGTCGATTTCGGTTTTGAATCCCTGATACTGGATCCATGCTCGTTCCGCTTGTTCTTCg                                                    >  2:527350/1‑140 (MQ=255)                                                                                           catcaAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGTTAGTGCCCTGGATGTTACGCAGGTAGATGTGTTTGTCGATTTCGGTTTTGAATCCCTGATACTGGATCCATGCTCGTTCCGCTTGTTCTTCGATGGtt                                              <  1:522695/140‑1 (MQ=255)                                                                                           catcaAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGTTAGTGCCCTGGATGTTACGCAGGTAGATGTGTTTGTCGATTTCGGTTTTGAATCCCTGATACTGGATCCATGCTCGTTCCGCTTGTTCTTCGATGGtt                                              <  2:65543/140‑1 (MQ=255)                                                                                            atcaAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGTTAGTGCCCTGGATGTTACGCAGGTAGATGTGTTTGTCGATTTCGGTTTTGAATCCCTGATACTGGATCCATGCTCGTTCCGCTTGTTCTTCGATGGttt                                             >  1:47290/1‑140 (MQ=255)                                                                                               aaGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGTTAGTGCCCTGGATGTTACGCAGGTAGATGTGTTTGTCGATTTCGGTTTTGAATCCCTGATACTGGATCCATGCTCGTTCCGCTTGTTCTTCGATGGTTTCGa                                          <  1:417131/140‑1 (MQ=255)                                                                                                aGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGTTAGTGCCCTGGATGTTACGCAGGTAGATGTGTTTGTCGATTTCGGTTTTGAATCCCTGATACTGGATCCATGCTCGTTCCGCTTGTTCTTCGATGGTTTCGAc                                         >  1:495814/1‑140 (MQ=255)                                                                                                 gatgatTGTTTACCAGACGGTAGAAGAGGGTTTCGTTAGTGCCCTGGATGTTACGCAGGTAGATGTGTTTGTCGATTTCGGTTTTGAATCCCTGATACTGGATCCATGCTCGTTCCGCTTGTTCTTCGATGGTTTCGAcc                                        <  1:527350/140‑1 (MQ=255)                                                                                                  atgatTGTTTACCAGACGGTAGAAGAGGGTTTCGTTAGTGCCCTGGATGTTACGCAGGTAGATGTGTTTGTCGATTTCGGTTTTGAATCCCTGATACTGGATCCATGCTCGTTCCGCTTGTTCTTCGATGGTTTCGACCa                                       >  1:579837/1‑140 (MQ=255)                                                                                                      ttGTTTACCAGACGGTAGAAGAGGGTTTCGTTAGTGCCCTGGATGTTACGCAGGTAGATGTGTTTGTCGATTTCGGTTTTGAATCCCTGATACTGGATCCATGCTCGTTCCGCTTGTTCTTCGATGGTTTCGACCACTTc                                   >  1:472878/1‑140 (MQ=255)                                                                                                      ttGTTTACCAGACGGTAGAAGAGGGTTTCGTTAGTGCCCTGGATGTTACGCAGGTAGATGTGTTTGTCGATTTCGGTTTTGAATCCCTGATACTGGATCCATGCTCGTTCCGCTTGTTCTTCGATGGTTTCGACCACTTc                                   >  1:362242/1‑140 (MQ=255)                                                                                                       tGTTTACCAGACGGTAGAAGAGGGTTTCGTTAGTGCCCTGGATGTTACGCAGGTAGATGTGTTTGTCGATTTCGGTTTTGAATCCCTGATACTGGATCCATGCTCGTTCCGCTTGTTCTTCGATGGTTTCGACCACTTcc                                  <  1:801260/140‑1 (MQ=255)                                                                                                               aGACGGTAGAAGAGGGTTTCGTTAGTGCCCTGGATGTTACGCAGGTAGATGTGTTTGTCGATTTCGGTTTTGAATCCCTGATACTGGATCCATGCTCGTTCCGCTTGTTCTTCGATGGTTTCGACCACTTCCGGCAGTaa                          >  2:693901/1‑140 (MQ=255)                                                                                                                 aCGGTAGAAGAGGGTTTCGTTAGTGCCCTGGATGTTACGCAGGTAGATGTGTTTGTCGATTTCGGTTTTGAATCCCTGATACTGGATCCATGCTCGTTCCGCTTGTTCTTCGATGGTTTCGACCACTTCCGGCAGTAAcc                        >  2:461542/1‑140 (MQ=255)                                                                                                                 aCGGTAGAAGAGGGTTTCGTTAGTGCCCTGGATGTTACGCAGGTAGATGTGTTTGTCGATTTCGGTTTTGAATCCCTGATACTGGATCCATGCTCGTTCCGCTTGTTCTTCGATGGTTTCGACCACTTCCGGCAGTAAcc                        >  2:315110/1‑140 (MQ=255)                                                                                                                 aCGGTAGAAGAGGGTTTCGTTAGTGCCCTGGATGTTACGCAGGTAGATGTGTTTGTCGATTTCGGTTTTGAATCCCTGATACTGGATCCATGCTCGTTCCGCTTGTTCTTCGATGGTTTCGACCACTTCCGGCAGTAAcc                        >  2:268279/1‑140 (MQ=255)                                                                                                                      agaagaGGGTTTCGTTAGTGCCCTGGATGTTACGCAGGTAGATGTGTTTGTCGATTTCGGTTTTGAATCCCTGATACTGGATCCATGCTCGTTCCGCTTGTTCTTCGATGGTTTCGACCACTTCCGGCAGTAACCCcagc                   <  2:555490/140‑1 (MQ=255)                                                                                                                        aagaGGGTTTCGTTAGTGCCCTGGATGTTACGCAGGTAGATGTGTTTGTCGATTTCGGTTTTGAATCCCTGATACTGGATCCATGCTCGTTCCGCTTGTTCTTCGATGGTTTCGACCACTTCCGGCAGTAACCCcagcag                 >  1:346798/1‑140 (MQ=255)                                                                                                                             ggTTTCGTTAGTGCCCTGGATGTTACGCAGGTAGATGTGTTTGTCGATTTCGGTTTTGAATCCCTGATACTGGATCCATGCTCGTTCCGCTTGTTCTTCGATGGTTTCGACCACTTCCGGCAGTAACCCca                     <  1:766371/131‑1 (MQ=255)                                                                                                                             ggTTTCGTTAGTGCCCTGGATGTTACGCAGGTAGATGTGTTTGTCGATTTCGGTTTTGAATCCCTGATACTGGATCCATGCTCGTTCCGCTTGTTCTTCGATGGTTTCGACCACTTCCGGCAGTAACCCca                     >  2:766371/1‑131 (MQ=255)                                                                                                                             ggTTTCGTTAGTGCCCTGGATGTTACGCAGGTAGATGTGTTTGTCGATTTCGGTTTTGAATCCCTGATACTGGATCCATGCTCGTTCCGCTTGTTCTTCGATGGTTTCGACCACTTCCGGCAGTAACCCCAGCAGGTTGa            >  2:386225/1‑140 (MQ=255)                                                                                                                             ggTTTCGTTAGTGCCCTGGATGTTACGCAGGTAGATGTGTTTGTCGATTTCGGTTTTGAATCCCTGATACTGGATCCATGCTCGTTCCGCTTGTTCTTCGATGGTTTCGACCACTTCCGGCAGTAACCCCAGCAGGTTGa            >  1:728508/1‑140 (MQ=255)                                                                                                                               tttCGTTAGTGCCCTGGATGTTACGCAGGTAGATGTGTTTGTCGATTTCGGTTTTGAATCCCTGATACTGGATCCATGCTCGTTCCGCTTGTTCTTCGATGGTTTCGACCACTTCCGGCAGTAACCCCAGCAGGTTGAAg          >  2:6616/1‑140 (MQ=255)                                                                                                                               tttCGTTAGTGCCCTGGATGTTACGCAGGTAGATGTGTTTGTCGATTTCGGTTTTGAATCCCTGATACTGGATCCATGCTCGTTCCGCTTGTTCTTCGATGGTTTCGACCACTTCCGGCAGTAACCCCAGCAGGTTGAAg          >  1:144434/1‑140 (MQ=255)                                                                                                                                  cGTTAGTGCCCTGGATGTTACGCAGGTAGATGTGTTTGTCGATTTCGGTTTTGAATCCCTGATACTGGATCCATGCTCGTTCCGCTTGTTCTTCGATGGTTTCGACCACTTCCGGCAGTAACCCCAGCAGGTTGAAGTTa       <  2:634952/140‑1 (MQ=255)                                                                                                                                    ttAGTGCCCTGGATGTTACGCAGGTAGATGTGTTTGTCGATTTCGGTTTTGAATCCCTGATACTGGATCCATGCTCGTTCCGCTTGTTCTTCGATGGTTTCGACCACTTCCGGCAGTAACCCCAGCAGGTTGAAGTTACg     <  2:346798/140‑1 (MQ=255)                                                                                                                                       gtgcCCTGGATGTTACGCAGGTAGATGTGTTTGTCGATTTCGGTTTTGAATCCCTGATACTGGATCCATGCTCGTTCCGCTTGTTCTTCGATGGTTTCGACCACTTCCGGCAGTAACCCCAGCAGGTTGAAGTTACGGCg  <  1:268279/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         AGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGTTAGTGTCCTGGATGTTACGCAGGTAGATGTGTTTGTCGATTTCGGTTTTGAATCCCTGATACTGGATCCATGCTCGTTCCGCTTGTTCTTCGATGGTTTCGACCACTTCCGGCAGTAACCCCAGCAGGTTGAAGTTACGGCG  >  NC_000913/1555288‑1555561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑