Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,575,914	T→C	Y569C (TAC→TGC)	yddB ←	putative TonB‑dependent receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,575,914	0	T	C	100.0%	219.6 / NA	61	Y569C (TAC→TGC)	yddB	putative TonB‑dependent receptor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/29);  total (32/29)

GTTCGGCCAGTTCAAAACTGAGGCTGAACGAATGCGTTTTGGTTTTGCCATCGTTGTTATATTCAGTAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAG  >  NC_000913/1575783‑1576048                                                                                                                                    |                                                                                                                                       gTTCGGCCAGTTCAAAACTGAGGCTGAACGAATGCGTTTTGGTTTTGCCATCGTTGTTATATTCAGTAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGCAAACATAg                                                                                                                                >  2:600981/1‑140 (MQ=255) gTTCGGCCAGTTCAAAACTGAGGCTGAACGAATGCGTTTTGGTTTTGCCATCGTTGTTATATTCAGTAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGCAAACATAg                                                                                                                                >  2:66103/1‑140 (MQ=255) gTTCGGCCAGTTCAAAACTGAGGCTGAACGAATGCGTTTTGGTTTTGCCATCGTTGTTATATTCAGTAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGCAAACATAg                                                                                                                                >  2:185301/1‑140 (MQ=255)  ttCGGCCAGTTCAAAACTGAGGCTGAACGAATGCGTTTTGGTTTTGCCATCGTTGTTATATTCAGTAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGCAAACATAGt                                                                                                                               >  2:628825/1‑140 (MQ=255)    cGGCCAGTTCAAAACTGAGGCTGAACGAATGCGTTTTGGTTTTGCCATCGTTGTTATATTCAGTAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGCAAACATAGttt                                                                                                                             >  2:399016/1‑140 (MQ=255)    cGGCCAGTTCAAAACTGAGGCTGAACGAATGCGTTTTGGTTTTGCCATCGTTGTTATATTCAGTAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGCAAACATAGttt                                                                                                                             >  2:735968/1‑140 (MQ=255)       ccAGTTCAAAACTGAGGCTGAACGAATGCGTTTTGGTTTTGCCATCGTTGTTATATTCAGTAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGCAAACATAGTTTGCa                                                                                                                          <  1:807055/140‑1 (MQ=255)       ccAGTTCAAAACTGAGGCTGAACGAATGCGTTTTGGTTTTGCCATCGTTGTTATATTCAGTAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGCAAACATAGTTTGCa                                                                                                                          <  1:66103/140‑1 (MQ=255)           ttCAAAACTGAGGCTGAACGAATGCGTTTTGGTTTTGCCATCGTTGTTATATTCAGTAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGCAAACATAGTTTGCAcgcg                                                                                                                      >  2:254845/1‑140 (MQ=255)           ttCAAAACTGAGGCTGAACGAATGCGTTTTGGTTTTGCCATCGTTGTTATATTCAGTAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGCAAACATAGTTTGCAcgcg                                                                                                                      <  1:185522/140‑1 (MQ=255)                     aGGCTGAACGAATGCGTTTTGGTTTTGCCATCGTTGTTATATTCAGTAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGCAAACATAGTTTGCACGCGCAATAACGtt                                                                                                            >  1:123338/1‑140 (MQ=255)                                     ttttggttttgCCATCGTTGTTATATTCAGTAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGCAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTctgc                                                                                            <  1:254845/140‑1 (MQ=255)                                                        gttATATTCAGTAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGCAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGtt                                                                         <  1:628825/140‑1 (MQ=255)                                                        gttATATTCAGTAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGCAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGtt                                                                         <  1:563922/140‑1 (MQ=255)                                                          tataTTCAGTAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGCAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCa                                                                       <  1:735968/140‑1 (MQ=255)                                                          tataTTCAGTAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGCAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCa                                                                       <  1:600981/140‑1 (MQ=255)                                                                cAGTAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTCTGCTGATTTGATCATGCGCTTCACGGCAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTta                                                                 >  2:353217/1‑140 (MQ=255)                                                                 aGTAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGCAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATc                                                                     >  2:411666/1‑135 (MQ=255)                                                                 aGTAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGCAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATc                                                                     <  1:411666/135‑1 (MQ=255)                                                                 aGTAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGCAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTtat                                                                >  1:384577/1‑140 (MQ=255)                                                                 aGTAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGCAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTtat                                                                >  1:279362/1‑140 (MQ=255)                                                                   tAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGCAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATaa                                                              >  2:215758/1‑140 (MQ=255)                                                                   tAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGCAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATaa                                                              >  2:708271/1‑140 (MQ=255)                                                                   tAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGCAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATaa                                                              >  2:773391/1‑140 (MQ=255)                                                                   tAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGCAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATaa                                                              >  2:788055/1‑140 (MQ=255)                                                                    aaTGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGCAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAg                                                             <  1:736033/140‑1 (MQ=255)                                                                     aTGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGCAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAgg                                                            <  1:215758/140‑1 (MQ=255)                                                                      tggtggTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGCAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGc                                                           >  2:234561/1‑140 (MQ=255)                                                                       ggtggtTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGCAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCg                                                          <  1:662993/140‑1 (MQ=255)                                                                       ggtggtTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGCAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCg                                                          <  2:279362/140‑1 (MQ=255)                                                                        gtggtTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGCAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGt                                                         >  1:630403/1‑140 (MQ=255)                                                                        gtggtTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGCAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGt                                                         >  2:493255/1‑140 (MQ=255)                                                                        gtggtTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGCAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGt                                                         >  1:246045/1‑140 (MQ=255)                                                                        gtggtTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGCAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGt                                                         >  2:436989/1‑140 (MQ=255)                                                                        gtggtTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGCAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGt                                                         >  1:243399/1‑140 (MQ=255)                                                                        gtggtTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGCAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGt                                                         >  2:373422/1‑140 (MQ=255)                                                                        gtggtTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGCAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGt                                                         >  2:233095/1‑140 (MQ=255)                                                                           gtTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGCAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGtttt                                                      <  1:472139/140‑1 (MQ=255)                                                                             tttAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGCAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCa                                                    <  1:708271/140‑1 (MQ=255)                                                                               tAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGCAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCaaa                                                  <  1:234561/140‑1 (MQ=255)                                                                                aGTCGCGCTGTCGGTACGACTGCTTCTGCTGATTTGATCATGCGCTTCACGGCAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAAt                                                 <  1:353217/140‑1 (MQ=255)                                                                                      gcTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGCAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGAt                                           >  1:201099/1‑140 (MQ=255)                                                                                              tACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGCAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTc                                   <  2:358597/140‑1 (MQ=255)                                                                                               aCGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGCAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCa                                  <  2:384577/140‑1 (MQ=255)                                                                                                cGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGCAAACATAGTTTGCACGCGCAATAACGTTCTCACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCag                                 <  1:384115/140‑1 (MQ=255)                                                                                                 gACTGCTTTTGCTGATTTGATCATGCGCTTCACGGCAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCaga                                <  2:243399/140‑1 (MQ=255)                                                                                                 gACTGCTTTTGCTGATTTGATCATGCGCTTCACGGCAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCaga                                <  2:630403/140‑1 (MQ=255)                                                                                                  aCTGCTTTTGCTGATTTGATCATGCGCTTCACGGCAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCagag                               >  1:171401/1‑140 (MQ=255)                                                                                                    tGCTTTTGCTGATTTGATCATGCGCTTCACGGCAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGtt                             >  1:289497/1‑140 (MQ=255)                                                                                                      cTTTTGCTGATTTGATCATGCGCTTCACGGCAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGtttt                           >  1:763373/1‑140 (MQ=255)                                                                                                      cTTTTGCTGATTTGATCATGCGCTTCACGGCAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGtttt                           >  1:640798/1‑140 (MQ=255)                                                                                                       ttttGCTGATTTGATCATGCGCTTCACGGCAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTtta                          <  1:773391/140‑1 (MQ=255)                                                                                                       ttttGCTGATTTGATCATGCGCTTCACGGCAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTtta                          <  1:399016/140‑1 (MQ=255)                                                                                                                  tGATCATGCGCTTCACGGCAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATAc               <  2:123338/140‑1 (MQ=255)                                                                                                                      cATGCGCTTCACGGCAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGAt           <  1:436989/140‑1 (MQ=255)                                                                                                                      cATGCGCTTCACGGCAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGAt           <  2:763373/140‑1 (MQ=255)                                                                                                                           gcTTCACGGCAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGt      <  2:795927/140‑1 (MQ=255)                                                                                                                             ttCACGGCAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTcc    <  1:493255/140‑1 (MQ=255)                                                                                                                             ttCACGGCAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTcc    >  1:403255/1‑140 (MQ=255)                                                                                                                               cACGGCAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAg  >  1:400440/1‑140 (MQ=255)                                                                                                                               cACGGCAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAg  >  2:784572/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                       GTTCGGCCAGTTCAAAACTGAGGCTGAACGAATGCGTTTTGGTTTTGCCATCGTTGTTATATTCAGTAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAG  >  NC_000913/1575783‑1576048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑