Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,583,276	T→C	K138E (AAA→GAA)	ydeO ←	DNA‑binding transcriptional dual regulator YdeO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,583,276	0	T	C	100.0%	146.7 / NA	41	K138E (AAA→GAA)	ydeO	DNA‑binding transcriptional dual regulator YdeO
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/26);  total (15/26)

AAGAATCTGTGAGAATGTCGTTTGCTCTTGCTTAAGTTTTTTCTTCAACAGGCTTTCACTGATGTACAGGCAGTCACAAATATCTTTCAGCTTCCATGGGTGCGCCGGCTTCATGTTGACAATATTTCTCACTTTCCCAGAAACGGATAGCACACCGTTAGTTAATAGTGTAATGAAACCTTTGCATGCGGCAAAAATAGACAGGCAAGAAAAAAGCAAAAGTTCTGAAAAATTATGATGATTGCGTTCTTCCGAGTTGA  >  NC_000913/1583142‑1583401                                                                                                                                       |                                                                                                                              aaGAATCTGTGAGAATGTCGTTTGCTCTTGCTTAAGTTTTTTCTTCAACAGGCTTTCACTGATGTACAGGCAGTCACAAATATCTTTCAGCTTCCATGGGTGCGCCGGCTTCATGTTGACAATATTTCTCACTTCCCCAg                                                                                                                          >  1:675806/1‑140 (MQ=255)   gAATCTGTGAGAATGTCGTTTGCTCTTGCTTAAGTTTTTTCTTCAACAGGCTTTCACTGATGTACAGGCAGTCACAAATATCTTTCAGCTTCCATGGGTGCGCCGGCTTCATGTTGACAATATTTCTCACTTCCCCAGaa                                                                                                                        <  2:614543/140‑1 (MQ=255)        tgtgAGAATGTCGTTTGCTCTTGCTTAAGTTTTTTCTTCAACAGGCTTTCACTGATGTACAGGCAGTCACAAATATCTTTCAGCTTCCATGGGTGCGCCGGCTTCATGTTGACAATATTTCTCACTTCCCCAGAAACGGa                                                                                                                   <  2:603730/140‑1 (MQ=255)          tgAGAATGTCGTTTGCTCTTGCTTAAGTTTTTTCTTCAACAGGCTTTCACTGATGTACAGGCAGTCACAAATATCTTTCAGCTTCCATGGGTGCGCCGGCTTCATGTTGACAATATTTCTCACTTCCCCAGAAACGGa                                                                                                                   >  1:364691/1‑138 (MQ=255)          tgAGAATGTCGTTTGCTCTTGCTTAAGTTTTTTCTTCAACAGGCTTTCACTGATGTACAGGCAGTCACAAATATCTTTCAGCTTCCATGGGTGCGCCGGCTTCATGTTGACAATATTTCTCACTTCCCCAGAAACGGa                                                                                                                   <  2:364691/138‑1 (MQ=255)             gaATGTCGTTTGCTCTTGCTTAAGTTTTTTCTTCAACAGGCTTTCACTGATGTACAGGCAGTCACAAATATCTTTCAGCTTCCATGGGTGCGCCGGCTTCATGTTGACAATATTTCTCACTTCCCCAGAAACGGATAGca                                                                                                              <  1:138413/140‑1 (MQ=255)             gaATGTCGTTTGCTCTTGCTTAAGTTTTTTCTTCAACAGGCTTTCACTGATGTACAGGCAGTCACAAATATCTTTCAGCTTCCATGGGTGCGCCGGCTTCATGTTGACAATATTTCTCACTTCCCCAGAAACGGATAGca                                                                                                              <  2:337144/140‑1 (MQ=255)             gaATGTCGTTTGCTCTTGCTTAAGTTTTTTCTTCAACAGGCTTTCACTGATGTACAGGCAGTCACAAATATCTTTCAGCTTCCATGGGTGCGCCGGCTTCATGTTGACAATATTTCTCACTTCCCCAGAAACGGATAGca                                                                                                              <  1:734160/140‑1 (MQ=255)              aaTGTCGTTTGCTCTTGCTTAAGTTTTTTCTTCAACAGGCTTTCACTGATGTACAGGCAGTCACAAATATCTTTCAGCTTCCATGGGTGCGCCGGCTTCATGTTGACAATATTTCTCACTTCCCCAGAAACGGATAg                                                                                                                >  1:703950/1‑137 (MQ=255)              aaTGTCGTTTGCTCTTGCTTAAGTTTTTTCTTCAACAGGCTTTCACTGATGTACAGGCAGTCACAAATATCTTTCAGCTTCCATGGGTGCGCCGGCTTCATGTTGACAATATTTCTCACTTCCCCAGAAACGGATAg                                                                                                                <  2:703950/137‑1 (MQ=255)                tGTCGTTTGCTCTTGCTTAAGTTTTTTCTTCAACAGGCTTTCACTGATGTACAGGCAGTCACAAATATCTTTCAGCTTCCATGGGTGCGCCGGCTTCATGTTGACAATATTTCTCACTTCCCCAGAAACGGATAGCacac                                                                                                           <  1:634303/140‑1 (MQ=255)                    gTTTGCTCTTGCTTAAGTTTTTTCTTCAACAGGCTTTCACTGATGTACAGGCAGTCACAAATATCTTTCAGCTTCCATGGGTGCGCCGGCTTCATGTTGACAATATTTCTCACTTCCCCAGAAACGGATAGCACACCgtt                                                                                                       >  2:747413/1‑140 (MQ=255)                    gTTTGCTCTTGCTTAAGTTTTTTCTTCAACAGGCTTTCACTGATGTACAGGCAGTCACAAATATCTTTCAGCTTCCATGGGTGCGCCGGCTTCATGTTGACAATATTTCTCACTTCCCCAGAAACGGATAGCACACCgtt                                                                                                       >  2:567173/1‑140 (MQ=255)                       tGCTCTTGCTTAAGTTTTTTCTTCAACAGGCTTTCACTGATGTACAGGCAGTCACAAATATCTTTCAGCTTCCATGGGTGCGCCGGCTTCATGTTGACAATATTTCTCACTTCCCCAGAAACGGATAGCACACCgttagt                                                                                                    <  1:608273/140‑1 (MQ=255)                          tctTGCTTAAGTTTTTTCTTCAACAGGCTTTCACTGATGTACAGGCAGTCACAAATATCTTTCAGCTTCCATGGGTGCGCCGGCTTCATGTTGACAATATTTCTCACTTCCCCAGAAACGGATAGCACACCGTTAGTTaa                                                                                                 <  2:675806/140‑1 (MQ=255)                            ttGCTTAAGTTTTTTCTTCAACAGGCTTTCACTGATGTACAGGCAGTCACAAATATCTTTCAGCTTCCATGGGTGCGCCGGCTTCATGTTGACAATATTTCTCACTTCCCCAGAAACGGATAGCACACCGTTAGTTAATa                                                                                               <  2:543976/140‑1 (MQ=255)                                    gTTTTTTCTTCAACAGGCTTTCACTGATGTACAGGCAGTCACAAATATCTTTCAGCTTCCATGGGTGCGCCGGCTTCATGTTGACAATATTTCTCACTTCCCCAGAAACGGATAGCACACCGTTAGTTAATAGTGTAATg                                                                                       <  1:567173/140‑1 (MQ=255)                                    gTTTTTTCTTCAACAGGCTTTCACTGATGTACAGGCAGTCACAAATATCTTTCAGCTTCCATGGGTGCGCCGGCTTCATGTTGACAATATTTCTCACTTCCCCAGAAACGGATAGCACACCGTTAGTTAATAGTGTAATg                                                                                       <  1:747413/140‑1 (MQ=255)                                     ttttttCTTCAACAGGCTTTCACTGATGTACAGGCAGTCACAAATATCTTTCAGCTTCCATGGGTGCGCCGGCTTCATGTTGACAATATTTCTCACTTCCCCAGAAACGGATAGCACACCGTTAGTTAATAGTGTAATGa                                                                                      <  1:252488/140‑1 (MQ=255)                                         ttcttcAACAGGCTTTCACTGATGTACAGGCAGTCACAAATATCTTTCAGCTTCCATGGGTGCGCCGGCTTCATGTTGACAATATTTCTCACTTCCCCAGAAACGGATAGCACACCGTTAGTTAATAGTGTAATGa                                                                                      >  1:533831/1‑136 (MQ=255)                                         ttcttcAACAGGCTTTCACTGATGTACAGGCAGTCACAAATATCTTTCAGCTTCCATGGGTGCGCCGGCTTCATGTTGACAATATTTCTCACTTCCCCAGAAACGGATAGCACACCGTTAGTTAATAGTGTAATGa                                                                                      <  2:533831/136‑1 (MQ=255)                                         ttcttcAACAGGCTTTCACTGATGTACAGGCAGTCACAAATATCTTTCAGCTTCCATGGGTGCGCCGGCTTCATGTTGACAATATTTCTCACTTCCCCAGAAACGGATAGCACACCGTTAGTTAATAGTGTAATGAAAcc                                                                                  >  1:454962/1‑140 (MQ=255)                                                aCAGGCTTTCACTGATGTACAGGCAGTCACAAATATCTTTCAGCTTCCATGGGTGCGCCGGCTTCATGTTGACAATATTTCTCACTTCCCCAGAAACGGATAGCACACCGTTAGTTAATAGTGTAATGAAACCTTTGCAt                                                                           <  1:645029/140‑1 (MQ=255)                                                    gCTTTCACTGATGTACAGGCAGTCACAAATATCTTTCAGCTTCCATGGGTGCGCCGGCTTCATGTTGACAATATTTCTCACTTCCCCAGAAACGGATAGCACACCGTTAGTTAATAGTGTAATGAAACCTTTGCATGCgg                                                                       <  1:590189/140‑1 (MQ=255)                                                     cTTTCACTGATGTACAGGCAGTCACAAATATCTTTCAGCTTCCATGGGTGCGCCGGCTTCATGTTGACAATATTTCTCACTTCCCCAGAAACGGATAGCACACCGTTAGTTAATAGTGTAATGAAACCTTTGCATGCGGc                                                                      <  1:291612/140‑1 (MQ=255)                                                       ttCACTGATGTACAGGCAGTCACAAATATCTTTCAGCTTCCATGGGTGCGCCGGCTTCATGTTGACAATATTTCTCACTTCCCCAGAAACGGATAGCACACCGTTAGTTAATAGTGTAATGAAACCTTTGCATGCGGCaa                                                                    <  1:760379/140‑1 (MQ=255)                                                                      gCAGTCACAAATATCTTTCAGCTTCCATGGGTGCGCCGGCTTCATGTTGACAATATTTCTCACTTCCCCAGAAACGGATAGCACACCGTTAGTTAATAGTGTAATGAAACCTTTGCATGCGGCaa                                                                    >  1:299629/1‑125 (MQ=255)                                                                      gCAGTCACAAATATCTTTCAGCTTCCATGGGTGCGCCGGCTTCATGTTGACAATATTTCTCACTTCCCCAGAAACGGATAGCACACCGTTAGTTAATAGTGTAATGAAACCTTTGCATGCGGCaa                                                                    <  2:299629/125‑1 (MQ=255)                                                                      gCAGTCACAAATATCTTTCAGCTTCCATGGGTGCGCCGGCTTCATGTTGACAATATTTCTCACTTCCCCAGAAACGGATAGCACACCGTTAGTTAATAGTGTAATGAAACCTTTGCATGCGGCAAAAATAGACAGGCAAg                                                     >  1:543507/1‑140 (MQ=255)                                                                      gCAGTCACAAATATCTTTCAGCTTCCATGGGTGCGCCGGCTTCATGTTGACAATATTTCTCACTTCCCCAGAAACGGATAGCACACCGTTAGTTAATAGTGTAATGAAACCTTTGCATGCGGCAAAAATAGACAGGCAAg                                                     >  2:120165/1‑140 (MQ=255)                                                                        aGTCACAAATATCTTTCAGCTTCCATGGGTGCGCCGGCTTCATGTTGACAATATTTCTCACTTCCCCAGAAACGGATAGCACACCGTTAGTTAATAGTGTAATGAAACCTTTGCATGCGGCAAAAATAGACAGGCAAGaa                                                   >  1:407771/1‑140 (MQ=255)                                                                        aGTCACAAATATCTTTCAGCTTCCATGGGTGCGCCGGCTTCATGTTGACAATATTTCTCACTTCCCCAGAAACGGATAGCACACCGTTAGTTAATAGTGTAATGAAACCTTTGCATGCGGCAAAAATAGACAGGCAAGaa                                                   >  2:747612/1‑140 (MQ=255)                                                                                atatCTTTCAGCTTCCATGGGTGCGCCGGCTTCATGTTGACAATATTTCTCACTTCCCCAGAAACGGATAGCACACCGTTAGTTAATAGTGTAATGAAACCTTTGCATGCGGCAAAAATAGACAGGCAAGAAAAAAGCaa                                           <  1:60870/140‑1 (MQ=255)                                                                                      ttCAGCTTCCATGGGTGCGCCGGCTTCATGTTGACAATATTTCTCACTTCCCCAGAAACGGATAGCACACCGTTAGTTAATAGTGTAATGAAACCTTTGCATGCGGCAAAAATAGACAGGCAAGAAAAAAGCAAAAGTTc                                     >  1:585845/1‑140 (MQ=255)                                                                                        cAGCTTCCATGGGCGCGCCGGCATCATGTTGACAATATTTCTCACTTCCCCAGAAACGGATAGCAGACCGTTAGTTAATAGTGTAATGAAACCTTTGCATGCGGCAAAAATAGACAGGCAAGAAAAAAGCAAAAGTTCTg                                   >  2:712599/1‑140 (MQ=255)                                                                                                 tGGGTGCGCCGGCTTCATGTTGACAATATTTCTCACTTCCCCAGAAACGGATAGCACACCGTTAGTTAATAGTGTAATGAAACCTTTGCATGCGGCAAAAATAGACAGGCAAGAAAAAAGCAAAAGTTCTGAAAAATTat                          >  2:565202/1‑140 (MQ=255)                                                                                                      gcgcCGGCTTCATGTTGACAATATTTCTCACTTCCCCAGAAACGGATAGCACACCGTTAGTTAATAGTGTAATGAAACCTTTGCATGCGGCAAAAATAGACAGGCAAGAAAAAAGCAAAAGTTCTGAAAAATTAtgatga                     <  1:47153/140‑1 (MQ=255)                                                                                                       cgcCGGCTTCATGTTGACAATATTTCTCACTTCCCCAGAAACGGATAGCACACCGTTAGTTAATAGTGTAATGAAACCTTTGCATGCGGCAAAAATAGACAGGCAAGAAAAAAGCAAAAGTTCTGAAAAATTATgatgat                    >  2:619632/1‑140 (MQ=255)                                                                                                           ggCTTCATGTTGACAATATTTCTCACTTCCCCAGAAACGGATAGCACACCGTTAGTTAATAGTGTAATGAAACCTTTGCATGCGGCAAAAATAGACAGGCAAGAAAAAAGCAAAAGTTCTGAAAAATTATGATGATTGCg                <  1:120165/140‑1 (MQ=255)                                                                                                           ggCTTCATGTTGACAATATTTCTCACTTCCCCAGAAACGGATAGCACACCGTTAGTTAATAGTGTAATGAAACCTTTGCATGCGGCAAAAATAGACAGGCAAGAAAAAAGCAAAAGTTCTGAAAAATTATGATGATTGCg                <  1:619632/140‑1 (MQ=255)                                                                                                                cATGTTGACAATATTTCTCACTTCCCCAGAAACGGATAGCACACCGTTAGTTAATAGTGTAATGAAACCTTTGCATGCGGCAAAAATAGACAGGCAAGAAAAAAGCAAAAGTTCTGAAAAATTATGATGATTGCGttctt           <  2:407771/140‑1 (MQ=255)                                                                                                                         aaTATTTCTCACTTCCCCAGAAACGGATAGCACACCGTTAGTTAATAGTGTAATGAAACCTTTGCATGCGGCAAAAATAGACAGGCAAGAAAAAAGCAAAAGTTCTGAAAAATTATGATGATTGCGTTCTTCCGAGTTga  <  1:375584/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                              AAGAATCTGTGAGAATGTCGTTTGCTCTTGCTTAAGTTTTTTCTTCAACAGGCTTTCACTGATGTACAGGCAGTCACAAATATCTTTCAGCTTCCATGGGTGCGCCGGCTTCATGTTGACAATATTTCTCACTTTCCCAGAAACGGATAGCACACCGTTAGTTAATAGTGTAATGAAACCTTTGCATGCGGCAAAAATAGACAGGCAAGAAAAAAGCAAAAGTTCTGAAAAATTATGATGATTGCGTTCTTCCGAGTTGA  >  NC_000913/1583142‑1583401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑