Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,625,704	C→T	M559I (ATG→ATA)	dcp ←	peptidyl‑dipeptidase Dcp

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,625,704	0	C	T	94.9%	121.5 / ‑1.6	39	M559I (ATG→ATA)	dcp	peptidyl‑dipeptidase Dcp
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (16/21);  total (17/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

CAAAAATATGGGCGAAATAACTGCTGCGATAGCGTGGCGGTATAGCAGGAAGATCCATATTTTCCGCCACCAGCGCCCGCAATTCAAAATCATCGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCATTACGCATTTTCTGTTGCAGTTCGTCAGGCATTGCTGCCCCGCTCTGATAATGCCGGGCGTAGCGAGCG  >  NC_000913/1625568‑1625836                                                                                                                                         |                                                                                                                                     caAAAATATGGGCGAAATAACTGCTGCGATAGCGTGGCGGTATAGCAGGAAGATCCATATTTTCCGCCACCAGCGCCCGCAATTCAAAATCATCGCCATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGtata                                                                                                                                   >  2:446292/1‑140 (MQ=255)   aaaaTATGGGCGAAATAACTGCTGCGATAGCGTGGCGGTATAGCAGGAAGATCCATATTTTCCGCCACCAGCGCCCGCAATTCAAAATCTTCGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGTATATc                                                                                                                                 >  2:234941/1‑140 (MQ=255)           ggCGAAATAACTGCTGCGATAGCGTGGCGGTATAGCAGGAAGATCCATATTTTCCGCCACCAGCGCCCGCAATTCAAAATCATCGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGTATATCGAGAAGTg                                                                                                                         <  2:453529/140‑1 (MQ=255)                  tAACTGCTGCGATAGCGTGGCGGTATAGCAGGAAGATCCATATTTTCCGCCACCAGCGCCCGCAATTCAAAATCATCGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGTATATCGAGAAGTGCGGCGCt                                                                                                                  <  1:395704/140‑1 (MQ=255)                          gcGATAGCGTGGCGGTATAGCAGGAAGATCCATATTTTCCGCCACCAGCGCCCGCAATTCAAAATCATCGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGTATATCGAGAAGTgcg                                                                                                                       >  2:465053/1‑127 (MQ=255)                          gcGATAGCGTGGCGGTATAGCAGGAAGATCCATATTTTCCGCCACCAGCGCCCGCAATTCAAAATCATCGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGTATATCGAGAAGTgcg                                                                                                                       <  1:465053/127‑1 (MQ=255)                             aTAGCGTGGCGGTATAGCAGGAAGATCCATATTTTCCGCCACCAGCGCCCGCAATTCAAAATCATCGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGTATATCGAGAAGTgcg                                                                                                                       <  1:268918/124‑1 (MQ=255)                             aTAGCGTGGCGGTATAGCAGGAAGATCCATATTTTCCGCCACCAGCGCCCGCAATTCAAAATCATCGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGTATATCGAGAAGTgcg                                                                                                                       >  2:268918/1‑124 (MQ=255)                             aTAGCGTGGCGGTATAGCAGGAAGATCCATATTTTCCGCCACCAGCGCCCGCAATTCAAAATCATCGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGTATATCGAGAAGTGCGGCGCTAAGCAGTTCGc                                                                                                       <  1:806879/140‑1 (MQ=255)                              tAGCGTGGCGGTATAGCAGGAAGATCCATATTTTCCGCCACCAGCGCCCGCAATTCAAAATCATCGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGTATATCTAGAAGTGCGGCGCTAAGCAGTTCGct                                                                                                      <  2:355931/140‑1 (MQ=255)                              tAGCGTGGCGGTATAGCAGGAAGATCCATATTTTCCGCCACCAGCGCCCGCAATTCAAAATCATCGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGTATATCGAGAAGTGCGGCGCTAAGCAGTTCGct                                                                                                      <  1:604949/140‑1 (MQ=255)                              tAGCGTGGCGGTATAGCAGGAAGATCCATATTTTCCGCCACCAGCGCCCGCAATTCAAAATCATCGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGTATATCGAGAAGTGCGGCGCTAAGCAGCTCGct                                                                                                      <  2:748075/140‑1 (MQ=255)                               aGCGTGGCGGTATAGCAGGAAGATCCATATTTTCCGCCACCAGCGCCCGCAATTCAAAATCATCGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGTATATCGAGAAGTGCGGCGCTAAGCAGTTCGctc                                                                                                     <  2:397627/140‑1 (MQ=255)                               aGCGTGGCGGTATAGCAGGAAGATCCATATTTTCCGCCACCAGCGCCCGCAATTCAAAATCATCGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGTATATCGAGAAGTGCGGCGCTAAGCAGTTCGctc                                                                                                     <  1:709442/140‑1 (MQ=255)                                   tGGCGGTATAGCAGGAAGATCCATATTTTCCGCCACCAGCGCCCGCAATTCAAAATCATCGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGTATATCGAGAAGTGCGGCGCTAAGCAGTTCGctcatct                                                                                                 >  1:325372/1‑140 (MQ=255)                                   tGGCGGTATAGCAGGAAGATCCATATTTTCCGCCACCAGCGCCCGCAATTCAAAATCATCGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGTATATCGAGAAGTGCGGCGCTAAGCAGTTCGctcatct                                                                                                 >  2:646141/1‑140 (MQ=255)                                     gCGGTATAGCAGGAAGATCCATATTTTCCGCCACCAGCGCCCGCAATTCAAAATCATCGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCg                                                                                                        >  2:251096/1‑131 (MQ=255)                                     gCGGTATAGCAGGAAGATCCATATTTTCCGCCACCAGCGCCCGCAATTCAAAATCATCGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCg                                                                                                        <  1:251096/131‑1 (MQ=255)                                         tataGCAGGAAGATCCATATTTTCCGCCACCAGCGCCCGCAATTCAAAATCATCGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGTATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATAcc                                                                                           <  1:626179/140‑1 (MQ=255)                                                   aGATCCATATTTTCCGCCACCAGCGCCCGCAATTCAAAATCATCGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGTATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATAcc                                                                                           <  2:210641/130‑1 (MQ=255)                                                   aGATCCATATTTTCCGCCACCAGCGCCCGCAATTCAAAATCATCGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGTATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATAcc                                                                                           >  1:210641/1‑130 (MQ=255)                                                   aGATCCATATTTTCCGCCACCAGCGCCCGCAATTCAAAATCATCGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGTATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGaa                                                                                 >  2:679516/1‑140 (MQ=255)                                                   aGATCCATATTTTCCGCCACCAGCGCCCGCAATTCAAAATCATCGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGTATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGaa                                                                                 <  2:172763/140‑1 (MQ=255)                                                   aGATCCATATTTTCCGCCACCAGCGCCCGCAATTCAAAATCATCGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGTATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGaa                                                                                 >  1:428119/1‑140 (MQ=255)                                                     aTCCATATTTTCCGCCACCAGCGCCCGCAATTCAAAATCATCGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGTATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATAcc                                                                                           >  1:805936/1‑128 (MQ=255)                                                     aTCCATATTTTCCGCCACCAGCGCCCGCAATTCAAAATCATCGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGTATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATAcc                                                                                           <  2:805936/128‑1 (MQ=255)                                                       ccATATTTTCCGCCACCAGCGCCCGCAATTCAAAATCTTCGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGTATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAgg                                                                             <  1:234941/140‑1 (MQ=255)                                                        cATATTTTCCGCCACCAGCGCCCGCAATTCAAAATCATCGACATCCTGCATTGCTTCGTTTTCTCCCAGGCAATGCCAGCGTATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGc                                                                            <  2:347054/140‑1 (MQ=255)                                                                    caccaGCGCCCGCAATTCAAAATCATCGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGTATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCATTACGCa                                                                <  1:673631/140‑1 (MQ=255)                                                                                                    ccTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGTATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCATTACGCATTTTCTGTTGCAGTTCGTCAGGCATTGCTGcc                                <  1:646141/140‑1 (MQ=255)                                                                                                    ccTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGTATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCATTACGCATTTTCTGTTGCAGTTCGTCAGGCATTGCTGcc                                >  1:16722/1‑140 (MQ=255)                                                                                                          ttGCTTCGTTTTCTTCCAGGCAATGCCAGCGTATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCATTACGCATTTTCTGTTGCAGTTCGTCAGGCATTGCTGCCCCGctc                          >  1:732945/1‑140 (MQ=255)                                                                                                             cTTCGTTTTCTTCCAGGCAATGCCAGCGTATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCATTACGCATTTTCTGTTGCAGTTCGTCAGGCATTGCTGCCCCGc                            <  2:89742/135‑1 (MQ=255)                                                                                                             cTTCGTTTTCTTCCAGGCAATGCCAGCGTATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCATTACGCATTTTCTGTTGCAGTTCGTCAGGCATTGCTGCCCCGc                            >  1:89742/1‑135 (MQ=255)                                                                                                             cTTCGTTTTCTTCCAGGCAATGCCAGCGTATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCATTACGCATTTTCTGTTGCAGTTCGTCAGGCATTGCTGCCCCGCTCTGa                       >  1:392074/1‑140 (MQ=255)                                                                                                                cGTTTTCTTCCAGGCAATGCCAGCGTATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCATTACGCATTTTCTGTTGCAGTTCGTCAGGCATTGCTGCCCCGCTCTGATaa                    >  2:578724/1‑140 (MQ=255)                                                                                                                  ttttCTTCCAGGCAATGCCAGCGTATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCATTACGCATTTTCTGTTGCAGTTCGTCAGGCATTGCTGCCCCGCTCTGATAATg                  >  2:568080/1‑140 (MQ=255)                                                                                                                            ggCAATGCCAGCGTATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCATTACGCATTTTCTGTTGCAGTTCGTCAGGCATTGCTGCCCCGCTCTGATAATGCCGGGCGTag        <  2:428119/140‑1 (MQ=255)                                                                                                                             gCAATGCCAGCGTATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCATTACGCATTTTCTGTTGCAGTTCGTCAGGCATTGCTGCCCCGCTCTGATAATGCCGGGCGTagc       <  1:704900/140‑1 (MQ=255)                                                                                                                                 tGCCAGCGTATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCATTACGCATTTTCTGTTGCAGTTCGTCAGGCATTGCTGCCCCGCTCTGATAATGCCGGGCGTagcgagc   >  2:295121/1‑140 (MQ=255)                                                                                                                                 tGCCAGCGTATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCATTACGCATTTTCTGTTGCAGTTCGTCAGGCATTGCTGCCCCGCTCTGATAATGCCGGGCGTagcgagc   >  1:130565/1‑140 (MQ=255)                                                                                                                                  gCCAGCGTATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCATTACGCATTTTCTGTTGCAGTTCGTCAGGCATTGCTGCCCCGCTCTGATAATGCCGGGCGTagcgagcg  <  1:55094/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     CAAAAATATGGGCGAAATAACTGCTGCGATAGCGTGGCGGTATAGCAGGAAGATCCATATTTTCCGCCACCAGCGCCCGCAATTCAAAATCATCGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCATTACGCATTTTCTGTTGCAGTTCGTCAGGCATTGCTGCCCCGCTCTGATAATGCCGGGCGTAGCGAGCG  >  NC_000913/1625568‑1625836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑