Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,701,234	G→A	G93D (GGC→GAC)	malY →	negative regulator of MalT activity/cystathionine beta‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,701,234	0	G	A	100.0%	79.2 / NA	23	G93D (GGC→GAC)	malY	negative regulator of MalT activity/cystathionine beta‑lyase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/12);  total (11/12)

AGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGT  >  NC_000913/1701099‑1701369                                                                                                                                        |                                                                                                                                        aGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGACCCt                                                                                                                                     >  1:488406/1‑140 (MQ=255) aGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGACCCt                                                                                                                                     >  1:661882/1‑140 (MQ=255) aGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGACCCt                                                                                                                                     >  2:543367/1‑140 (MQ=255) aGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGACCCt                                                                                                                                     >  1:793190/1‑140 (MQ=255)   gcgcTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGACCCTTc                                                                                                                                   <  1:322454/140‑1 (MQ=255)   gcgcTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGACCCTTc                                                                                                                                   >  1:727470/1‑140 (MQ=255)    cgcTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGACCCTTCt                                                                                                                                  <  1:543367/140‑1 (MQ=255)      cTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGACCCTTCTGt                                                                                                                                <  2:552359/140‑1 (MQ=255)      cTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGACCCTTCTGt                                                                                                                                >  1:552359/1‑140 (MQ=255)      cTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGACCCTTCTGt                                                                                                                                <  1:592593/140‑1 (MQ=255)         aaTCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGACCCTTCTGTCAt                                                                                                                             <  1:267288/140‑1 (MQ=255)               cgcCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGACCCTTCTGTCATCTatat                                                                                                                       <  1:447225/140‑1 (MQ=255)                        cACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGACCCTTCTGTCATCTATATGGTTTCAGa                                                                                                              <  2:569094/140‑1 (MQ=255)                             cGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGACCCTTCTGTCATCTATATGGTTTCAGAACTGa                                                                                                         <  1:773278/140‑1 (MQ=255)                                     gCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGACCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAg                                                                                                 >  1:697059/1‑140 (MQ=255)                                         cAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGACCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGt                                                                                             <  2:661882/140‑1 (MQ=255)                                                        cGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGACCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAg                                                                              <  2:378178/140‑1 (MQ=255)                                                          aTGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGACCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGc                                                                            <  2:361046/140‑1 (MQ=255)                                                          aTGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGACCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGc                                                                            >  1:385035/1‑140 (MQ=255)                                                            gAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGACCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCgt                                                                          >  2:530402/1‑140 (MQ=255)                                                             aGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGACCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCgtg                                                                         <  2:385035/140‑1 (MQ=255)                                                                                        ttcccccccAGCATTCCCCCGCCATCGATTCTCAGACGGTGGTGTATGCCCCTTCTGTCATTTATTTGTTTTCAGAACTGATTCGTCAGTGGTCTGAAACGGGTGAGGGGGTGGTGATCCCCACCCCCGCCTATGACGCa                                              <  2:488406/134‑1 (MQ=255)                                                                                             ccccAGCATTACACCGCCATCGTTTCTCAGGCGGTGGTGTATGCCCCTTCTGTCATTTATATGTTTTCAGAACTGATTCGTCAGGGGTCTGAAACGGGGGAGGGCGTGGTGATCCACACCCCCGCCTATGACGCATTTTa                                         <  2:727470/139‑1 (MQ=255)                                                                                                   cATTACACCGCCATCGATTCTCAGACGGTGGTGTATGACCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGc                                   >  1:808211/1‑140 (MQ=255)                                                                                                             ccATCGATTCTCAGACGGTGGTGTATGACCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGt                         >  2:743378/1‑140 (MQ=255)                                                                                                                  gATTCTCAGACGGTGGTGTATGCCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACGGGTGAGGGCGTGGTGACCCACACCCCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCa                    <  2:82723/140‑1 (MQ=255)                                                                                                                                    tATGCCCCTTCTGTCATTTATATGTTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACCCCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGt  <  2:697059/140‑1 (MQ=255)                                                                                                                                    tATGCCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAGGGGGTGGTGACCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGt  <  2:297966/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        AGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGT  >  NC_000913/1701099‑1701369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑