Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	120,164	A→G	intergenic (+29/+14)	ampE → / ← aroP	protein AmpE/aromatic amino acid:H(+) symporter AroP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	120,164	0	A	G	100.0%	170.8 / NA	49	intergenic (+29/+14)	ampE/aroP	protein AmpE/aromatic amino acid:H(+) symporter AroP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/27);  total (22/27)

TAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCAC  >  NC_000913/120033‑120300                                                                                                                                    |                                                                                                                                         tAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGGGGGCGTag                                                                                                                                  <  2:609533/140‑1 (MQ=255) tAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGGGGGCGTag                                                                                                                                  <  2:682925/140‑1 (MQ=255)    ggTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGGGGGCGTagaga                                                                                                                               >  2:635582/1‑140 (MQ=255)             gCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGGGGGCGTAGAGAGATTAATgc                                                                                                                      >  2:178284/1‑140 (MQ=255)               cGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTTAACAAGCGGGGGCGTAGAGAGATTAATgcgc                                                                                                                    >  2:566133/1‑140 (MQ=255)                   ggCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGGGGGCGTAGAGAGATTAATGCGCtttt                                                                                                                >  2:777938/1‑140 (MQ=255)                                     gaagaaAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGGGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGttttt                                                                                              >  2:251312/1‑139 (MQ=255)                                           aaCCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGGGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTa                                                                                        <  2:260587/140‑1 (MQ=255)                                             ccTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGGGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTaaa                                                                                      >  2:537460/1‑140 (MQ=255)                                                cgttcgtGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGGGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAg                                                                                   <  1:34983/140‑1 (MQ=255)                                                 gttcgtGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGGGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGa                                                                                  <  2:336675/140‑1 (MQ=255)                                                 gttcgtGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGGGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGa                                                                                  <  2:184469/140‑1 (MQ=255)                                                 gttcgtGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGGGGGCGTAGAGAGATTAATGCACTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGa                                                                                  <  2:14346/140‑1 (MQ=255)                                                  ttcgtGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGGGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGAt                                                                                 <  2:425658/140‑1 (MQ=255)                                                       ggtcgtggtGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGGGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCg                                                                            <  1:675778/140‑1 (MQ=255)                                                       ggtcgtggtGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGGGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCg                                                                            <  1:334117/140‑1 (MQ=255)                                                       ggtcgtggtGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGGGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCg                                                                            <  1:777938/140‑1 (MQ=255)                                                        gtcgtggtGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGGGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGa                                                                           >  2:277588/1‑140 (MQ=255)                                                           gtggtggtgATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGGGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATAc                                                                        >  2:764123/1‑140 (MQ=255)                                                               tggtgATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGGGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTaa                                                                    <  1:566133/140‑1 (MQ=255)                                                                 gtgATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGGGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAaca                                                                  >  2:304727/1‑140 (MQ=255)                                                                   gATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGGGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACg                                                                <  1:537460/140‑1 (MQ=255)                                                                      tGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGGGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATa                                                                         >  2:710248/1‑128 (MQ=255)                                                                      tGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGGGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATa                                                                         <  1:710248/128‑1 (MQ=255)                                                                       gCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGGGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCa                                                            >  2:361245/1‑140 (MQ=255)                                                                       gCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGGGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCa                                                            >  1:384512/1‑140 (MQ=255)                                                                        cACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGGGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAg                                                           <  2:542917/140‑1 (MQ=255)                                                                             ctGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGGGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATa                                                      <  1:685382/140‑1 (MQ=255)                                                                             ctGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGGGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATa                                                      <  1:361245/140‑1 (MQ=255)                                                                                aCGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGGGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCg                                                   <  1:9247/140‑1 (MQ=255)                                                                                  gATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGGGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCACACCggg                                                 <  2:384512/140‑1 (MQ=255)                                                                                    tttACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGGGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGAt                                               <  2:807689/140‑1 (MQ=255)                                                                                    tttACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGGGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGAt                                               <  1:41132/140‑1 (MQ=255)                                                                                     ttACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGGGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATc                                              >  1:747233/1‑140 (MQ=255)                                                                                       aCGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGGGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAg                                            >  2:293763/1‑140 (MQ=255)                                                                                       aCGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGGGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAg                                            >  2:660376/1‑140 (MQ=255)                                                                                       aCGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGGGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAg                                            <  1:304727/140‑1 (MQ=255)                                                                                       aCGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGGGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAg                                            >  2:728244/1‑140 (MQ=255)                                                                                                 ggTGTAAAGATTATTGCCCTCACCCTGTACGGGTGGGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGaa                                  <  1:323886/140‑1 (MQ=255)                                                                                                      aaaGATTATTGCCCTCACCCTGTACGGGTGGGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGc                             <  2:747233/140‑1 (MQ=255)                                                                                                           ttattGCCCTCACCCTGTACGGGTGGGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTc                        <  1:728244/140‑1 (MQ=255)                                                                                                                   cTCACCCTGTACGGGTGGGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGtc                >  1:212177/1‑140 (MQ=255)                                                                                                                     cACCCTGTACGGGTGGGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGtcat              <  2:353884/140‑1 (MQ=255)                                                                                                                            tACGGGTGGGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATa       >  2:409926/1‑140 (MQ=255)                                                                                                                             aCGGGTGGGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGtcatca            >  2:719417/1‑134 (MQ=255)                                                                                                                             aCGGGTGGGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGtcatca            <  1:719417/134‑1 (MQ=255)                                                                                                                                ggTGGGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCa   >  1:186158/1‑140 (MQ=255)                                                                                                                                ggTGGGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGAAACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCa   >  1:115928/1‑140 (MQ=255)                                                                                                                                 gTGGGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCAc  >  1:669349/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                         TAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCAC  >  NC_000913/120033‑120300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑