Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,810,080	T→C	intergenic (+32/‑131)	hxpB → / → ydjM	hexitol phosphatase B/putative inner membrane protein regulated by LexA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,810,080	0	T	C	100.0%	138.9 / NA	39	intergenic (+32/‑131)	hxpB/ydjM	hexitol phosphatase B/putative inner membrane protein regulated by LexA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/24);  total (15/24)

AAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAGTGAGTTTTATCATGA  >  NC_000913/1809963‑1810214                                                                                                                      |                                                                                                                                       aaGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGCTCTTTATCTGAAATTGCATTCa                                                                                                                  >  2:146821/1‑140 (MQ=255)                         tATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGCTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTa                                                                                          >  1:208404/1‑140 (MQ=255)                              gCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGCTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAgaga                                                                                     >  2:607645/1‑140 (MQ=255)                              gCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGCTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAgaga                                                                                     <  1:660141/140‑1 (MQ=255)                                 gACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGCTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAag                                                                                  >  1:693034/1‑140 (MQ=255)                                   cGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGCTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGaa                                                                                    <  1:450753/136‑1 (MQ=255)                                   cGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGCTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGaa                                                                                    >  2:450753/1‑136 (MQ=255)                                   cGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGCTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAagag                                                                                >  2:287320/1‑140 (MQ=255)                                   cGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGCTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAagag                                                                                >  1:507954/1‑140 (MQ=255)                                      cAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGCTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTa                                                                             <  1:287320/140‑1 (MQ=255)                                               aTCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGCTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTcac                                                                    <  2:416051/140‑1 (MQ=255)                                                 cGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGCTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTcacca                                                                  <  1:146821/140‑1 (MQ=255)                                                 cGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGCTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTcacca                                                                  <  1:807282/140‑1 (MQ=255)                                                 cGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGCTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTcacca                                                                  <  2:426984/140‑1 (MQ=255)                                                 cGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGCTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTcacca                                                                  <  2:693034/140‑1 (MQ=255)                                                         gAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGCTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTg                                                          <  1:553162/140‑1 (MQ=255)                                                         gAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGCTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTg                                                          <  1:330264/140‑1 (MQ=255)                                                         gAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGCTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTg                                                          <  2:335447/140‑1 (MQ=255)                                                           aCTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGCTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCa                                                        <  2:474196/140‑1 (MQ=255)                                                           aCTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGCTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCa                                                        <  1:236438/140‑1 (MQ=255)                                                            cTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGCTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCAc                                                       <  2:208404/140‑1 (MQ=255)                                                                ccGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGCTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCa                                                        >  2:796169/1‑135 (MQ=255)                                                                ccGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGCTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCa                                                        <  1:796169/135‑1 (MQ=255)                                                                   cAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGCTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATaa                                                <  2:157637/140‑1 (MQ=255)                                                                   cAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGCTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATaa                                                <  2:508835/140‑1 (MQ=255)                                                                    aaaaGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGCTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATaaa                                               <  1:524949/140‑1 (MQ=255)                                                                        gACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGCTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATc                                           <  2:239529/140‑1 (MQ=255)                                                                             tctcGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGCTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCtata                                      <  2:171301/140‑1 (MQ=255)                                                                              ctcGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGCTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATAc                                     >  1:524605/1‑140 (MQ=255)                                                                                       tGACCAGGGGCAGTGATCTCGCTGCCCCTGGCTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTAt                            <  1:3692/140‑1 (MQ=255)                                                                                         aCCAGGGGCAGTGATCTCGCTGCCCCTGGCTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCg                          <  2:154493/140‑1 (MQ=255)                                                                                             ggggCAGTGATCTCGCTGCCCCTGGCTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTAc                                <  2:545571/130‑1 (MQ=255)                                                                                             ggggCAGTGATCTCGCTGCCCCTGGCTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTAc                                >  1:545571/1‑130 (MQ=255)                                                                                             ggggCAGTGATCTCGCTGCCCCTGGCTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAg                      >  1:661564/1‑140 (MQ=255)                                                                                             ggggCAGTGATCTCGCTGCCCCTGGCTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAg                      >  2:263405/1‑140 (MQ=255)                                                                                                   gTGATCTCGCTGCCCCTGGCTCTTTATCTGAAGTTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAGt                >  1:774863/1‑140 (MQ=255)                                                                                                   gTGATCTCGCTGCCCCTGGCTCTCTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAGt                >  1:317738/1‑140 (MQ=255)                                                                                                              gCCCCTGGCTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAGTGAGTTTTATCa     >  1:262346/1‑140 (MQ=255)                                                                                                                 ccTGGCTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAGTGAGTTTTATCATGa  <  1:748704/140‑1 (MQ=255)                                                                                                                      |                                                                                                                                       AAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAGTGAGTTTTATCATGA  >  NC_000913/1809963‑1810214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑