| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,827,021 | G→A | D413D (GAC→GAT) | astB ← | N‑succinylarginine dihydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,827,021 | 0 | G | A | 92.9% | 79.5 / ‑1.2 | 28 | D413D (GAC→GAT) | astB | N‑succinylarginine dihydrolase |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (15/11); total (16/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.04e-01 | |||||||||||
TTTTACCCGTTAAGGTCAGAGCAAGAAAATTATCCATTGCCCCCTCCCTCGCGCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCGCCCGGCGTTCTTCT > NC_000913/1826887‑1827138 | ttttACCCGTTAAGGTCAGAGCAAGAAAATTATCCATTGCCCCCTCCCTCGCGCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGATCGGc < 2:257406/140‑1 (MQ=255) aaGGTCAGAGCAAGAAAATTATCCATTGCCCCCTCCCTCGCGCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGATCGGCCAGATCGgcag < 2:644260/140‑1 (MQ=255) gAAAATTATCCATTGCCCCCTCCCTCGCGCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGATCGGCCAGATCGGCAGCAGTAAGGCGATc > 2:327194/1‑140 (MQ=255) aTTGCCCCCTCCCTCGCGCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGATCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAAc > 1:486804/1‑140 (MQ=255) ccctccctCGCGCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGATCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCa < 1:346391/140‑1 (MQ=255) cctccctcGCGCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGATCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCAc > 1:509817/1‑140 (MQ=255) cctccctcGCGCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACAT‑CAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGATCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCAcc < 2:85015/140‑1 (MQ=255) cgcgCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGATCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCa > 2:112469/1‑140 (MQ=255) cTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGATCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGa < 2:492266/140‑1 (MQ=255) gAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGATCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGa < 2:467524/137‑1 (MQ=255) gAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGATCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGa > 1:467524/1‑137 (MQ=255) gAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGATCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAgcg > 1:506395/1‑140 (MQ=255) cgaaccgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGATCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCg < 1:575257/140‑1 (MQ=255) accgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGATCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACTCAGTCATTGAGCGCATTAAACAGCGTAt < 1:651713/140‑1 (MQ=255) accgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGATCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTAt > 2:457078/1‑140 (MQ=255) accgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGATCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTAt < 1:417275/140‑1 (MQ=255) tGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGATCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTtcatcatca > 1:52368/1‑136 (MQ=255) tGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGATCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTtcatcatca < 2:52368/136‑1 (MQ=255) ggCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGATCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCAccgcc > 1:118441/1‑140 (MQ=255) tCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGATCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGa > 1:138030/1‑140 (MQ=255) gTGCTTCCCGCCCTTCGCGCAGCAATTGCGGATCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTc > 1:637237/1‑131 (MQ=255) gTGCTTCCCGCCCTTCGCGCAGCAATTGCGGATCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTc < 2:637237/131‑1 (MQ=255) tGCTTCCCGCCCTTCGCGCAGCAATTGCGGATCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCGCCCGGc > 1:653637/1‑140 (MQ=255) gCTTCCCGCCCTTCGCGCAGCAATTGCGGATCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCGCCCGGCg > 2:315976/1‑140 (MQ=255) cccGCCCTTCGCGCAGCAATTGCGGATCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCGCCCGGCGttct > 1:611350/1‑140 (MQ=255) cccGCCCTTCGCGCAGCAATTGCGGATCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCGCCCGGCGttct > 1:290802/1‑140 (MQ=255) ccGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCGCCCGGCGttctt > 1:27204/1‑140 (MQ=255) gCCCTTCGCGCAGCAATTGCGGGTCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCGCCCGGCGttcttct < 2:27204/140‑1 (MQ=255) | TTTTACCCGTTAAGGTCAGAGCAAGAAAATTATCCATTGCCCCCTCCCTCGCGCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCGCCCGGCGTTCTTCT > NC_000913/1826887‑1827138 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |