Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,835,096	T→C	W97R (TGG→CGG)	ydjZ →	DedA family protein YdjZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,835,096	0	T	C	100.0%	103.7 / NA	30	W97R (TGG→CGG)	ydjZ	DedA family protein YdjZ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/14);  total (16/14)

GCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTT  >  NC_000913/1834968‑1835228                                                                                                                                 |                                                                                                                                     gCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCCGGGGCGGCTTg                                                                                                                           >  1:87021/1‑140 (MQ=255)   tttATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCCGGGGCGGCTTGCt                                                                                                                         >  1:67427/1‑140 (MQ=255)      aTTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCCGGGGCGGCTTGCTGTc                                                                                                                      <  2:67427/140‑1 (MQ=255)            tCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCTCTACCTGCGTTTTTGATCACCTTTGCCAATGCGCCGCTGTTTGGCGCGTTCCGGGCCGGCTTGCTGTCGTGGAc                                                                                                                >  1:300243/1‑140 (MQ=255)                        cTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCCGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGc                                                                                                    <  2:408838/140‑1 (MQ=255)                         tGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCCGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGcc                                                                                                   >  2:490553/1‑140 (MQ=255)                           gcggcAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCCGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCcgg                                                                                                 >  2:430829/1‑140 (MQ=255)                                aGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCCGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCcggcgtgg                                                                                            >  2:671758/1‑140 (MQ=255)                                   tGTCTCGTTCTTGTTGATGATTCTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCCGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCggcgcggcgc                                                                                         <  1:573147/140‑1 (MQ=255)                                     tctcGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCCGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCtg                                                                                       >  2:469260/1‑140 (MQ=255)                                     tctcGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCCGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCtg                                                                                       >  2:12792/1‑140 (MQ=255)                                     tctcGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCCGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCtg                                                                                       <  2:29939/140‑1 (MQ=255)                                              tgttgATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCCGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCtttttt                                                                              >  2:643985/1‑140 (MQ=255)                                               gttgATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCCGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTa                                                                             <  1:464758/140‑1 (MQ=255)                                                ttgATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCCGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTAt                                                                            <  2:300243/140‑1 (MQ=255)                                                  gatgatTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCCGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCg                                                                          <  1:72632/140‑1 (MQ=255)                                                       ttttGCAGGCCATTGCTGCACCGATACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCCGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCaga                                                                     >  1:35002/1‑140 (MQ=255)                                                                 cATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCCGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGcc                                                           <  1:12792/140‑1 (MQ=255)                                                                    tgctgcACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCCGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCcgcg                                                        <  1:778021/140‑1 (MQ=255)                                                                              cTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCCGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGa                                              >  1:514992/1‑140 (MQ=255)                                                                                   tGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCCGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAAt                                         >  1:654684/1‑140 (MQ=255)                                                                                      gTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCCGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTaa                                      >  1:360777/1‑140 (MQ=255)                                                                                      gTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCCGGGGCGGCTTGCTGTCGTGGACCAGCTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAGAATTaa                                      >  2:336734/1‑140 (MQ=255)                                                                                                 ccTTTGCCAATGCGTCGCTGTTTGGCGCGTTCCGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGTGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAAcc                           <  1:671758/140‑1 (MQ=255)                                                                                                 ccTTTGCCAATGCGTCGCTGTTTGGCGCGTTCCGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAAcc                           <  1:154578/140‑1 (MQ=255)                                                                                                    ttGCCAATGCGTCGCTGTTTGGCGCGTTCCGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTg                        <  1:430829/140‑1 (MQ=255)                                                                                                       ccAATGCGTCGCTGTTTGGCGCGTTCCGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCtt                     <  2:584543/140‑1 (MQ=255)                                                                                                       ccAATGCGTCGCTGTTTGGCGCGTTCCGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCtt                     <  2:654684/140‑1 (MQ=255)                                                                                                            gCGTCGCTGTTTGGCGCGTTCCGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAg                >  1:718948/1‑140 (MQ=255)                                                                                                               tCGCTGTTTGGCGCGTTCCGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTAt             >  2:419070/1‑140 (MQ=255)                                                                                                                          cgcgTTCCGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCtttt  >  2:695766/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                                     GCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTT  >  NC_000913/1834968‑1835228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑