Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,835,696	T→C	V61A (GTA→GCA)	ynjA →	AhpD‑like domain‑containing protein YnjA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,835,696	0	T	C	100.0%	107.8 / NA	31	V61A (GTA→GCA)	ynjA	AhpD‑like domain‑containing protein YnjA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/12);  total (19/12)

GTGGCTGCATCGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGGATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGGTAGTACGATCGCTTGTCAGCGCGCGCATTGCGCAAATGTGCCTGTGTGAGTTTTGTGTGGATATCACCAGTATGAAAGTCGCCGAGCGCACCGGCAGCAGCGATAAACTGCTGGCAGTGGCTGACTGGCG  >  NC_000913/1835565‑1835825                                                                                                                                    |                                                                                                                                  gTGGCTGCATCGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGGATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGGCAGTACGAt                                                                                                                           >  2:210929/1‑140 (MQ=255)  tGGCTGCATCGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGGATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGGCAGTACGATc                                                                                                                          >  1:64866/1‑140 (MQ=255)      tGCATCGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGGATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGGCAGTACGATCGCtt                                                                                                                      >  2:564503/1‑140 (MQ=255)             tcgCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGGATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGGCAGTACGATCGCTTGTCAgcg                                                                                                               >  2:508103/1‑140 (MQ=255)                 cATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGGATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGGCAGTACGATCGCTTGTCAgcgcgcg                                                                                                           <  1:684645/140‑1 (MQ=255)                       ggCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGGATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGGCAGTACGATCGCTTGTCAGCGCGCGCATTgc                                                                                                     <  1:564503/140‑1 (MQ=255)                                  aaGCCCCATTCGCTGGTGGGGGCGGATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGGCAGTACGATCGCTTGTCAGCGCGCGCATTGCGCAAATGTGcc                                                                                          <  2:64866/140‑1 (MQ=255)                                   aGCCCCATTCGCTGGTGGGGGCGGATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGGCAGTACGATCGCTTGTCAGCGCGCGCa                                                                                                         <  2:683267/124‑1 (MQ=255)                                   aGCCCCATTCGCTGGTGGGGGCGGATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGGCAGTACGATCGCTTGTCAGCGCGCGCa                                                                                                         >  1:683267/1‑124 (MQ=255)                                        cATTCGCTGGTGGGGGCGGATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGGCAGTACGATCGCTTGTCAGCGCGCGCATTGCGCAAATGTGCCtgtgtg                                                                                    >  2:91064/1‑140 (MQ=255)                                        cATTCGCTGGTGGGGGCGGATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGGCAGTACGATCGCTTGTCAGCGCGCGCATTGCGCAAATGTGCCtgtgtg                                                                                    >  1:339106/1‑140 (MQ=255)                                          ttCGCTGGTGGCGGCGGAGCCCGTTTATCTTTTATCTCGTGTCGATGTTGGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGGCAGTACGATCGCTTGTCAGCGCGCGCATTGCGCAAATGTGCCTGTGTGAg                                                                                  >  2:792104/1‑140 (MQ=255)                                                   gggggCGGATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGGCAGTACGATCGCTTGTCAGCGCGCGCATTGCGCAAATGTGCCTGTGTGAGTTTtgtgtg                                                                         <  2:339106/140‑1 (MQ=255)                                                    ggggCGGATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGGCAGTACGATCGCTTGTCAGCGCGCGCATTGCGCAAATGTGCCTGTGTGAGTTTTGTGTgg                                                                        >  1:134786/1‑140 (MQ=255)                                                       gCGGATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGGCAGTACGATCGCTTGTCAGCGCGCGCATTGCGCAAATGTGCCTGTGTGAGTTTTGTGTGGata                                                                     <  1:242620/140‑1 (MQ=255)                                                        cGGATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGGCAGTACGATCGCTTGTCAGCGCGCGCATTGCGCAAATGTGCCTGTGTGAGTTTTGTGTGGatat                                                                    <  1:508103/140‑1 (MQ=255)                                                            tCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGGCAGTACGATCGCTTGTCAGCGCGCGCATTGCGCAAATGTGCCTGTGTGAGTTTTGTGTGGATATCAcc                                                                >  2:41162/1‑140 (MQ=255)                                                             cccGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGGCAGTACGATCGCTTGTCAGCGCGCGCATTGCGCAAATGTGCCTGTGTGAGTTTTGTGTGGATATCACCa                                                               <  1:128505/140‑1 (MQ=255)                                                                        tttatctGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGGCAGTACGATCGCTTGTCAGCGCGCGCATTGCGCAAATGTGCCTGTGTGAGTTTTGTGTGGATATCACCAGTATGAAAGTc                                                    >  2:15742/1‑140 (MQ=255)                                                                           atctGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGGCAGTACGATCGCTTGTCAGCGCGCGCATTGCGCAAATGTGCCTGTGTGAGTTTTGTGTGGATATCACCAGTATGAAAGTCGcc                                                 >  1:551359/1‑140 (MQ=255)                                                                                gtgtCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGGCAGTACGATCGCTTGTCAGCGCGCGCATTGCGCAAATGTGCCTGTGTGAGTTTTGTGTGGATATCACCAGTATGAAAGTCGCCGAgcg                                            >  2:190288/1‑140 (MQ=255)                                                                                gtgtCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGGCAGTACGATCGCTTGTCAGCGCGCGCATTGCGCAAATGTGCCTGTGTGAGTTTTGTGTGGATATCACCAGTATGAAAGTCGCCGAgcg                                            >  2:622975/1‑140 (MQ=255)                                                                                   tCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGGCAGTACGATCGCTTGTCAGCGCGCGCATTGCGCAAATGTGCCTGTGTGAGTTTTGTGTGGATATCACCAGTATGAAAGTCGCCGAGCGCAc                                         >  1:85465/1‑140 (MQ=255)                                                                                           tgttgGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGGCAGTACGATCGCTTGTCAGCGCGCGCATTGCGCAAATGTGCCTGTGTGAGTTTTGTGTGGATATCACCAGTATGAAAGTCGCCGAGCGCACCGgcag                                   <  2:24997/138‑1 (MQ=255)                                                                                           tgttgGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGGCAGTACGATCGCTTGTCAGCGCGCGCATTGCGCAAATGTGCCTGTGTGAGTTTTGTGTGGATATCACCAGTATGAAAGTCGCCGAGCGCACCGgcag                                   >  1:24997/1‑138 (MQ=255)                                                                                                 ctggctggAGCGCAAACGCTCACCGCTCGATCCGGCAGTACGATCGCTTGTCAGCGCGCGCATTGCGCAAATGTGCCTGTGTGAGTTTTGTGTGGATATCACCAGTATGAAAGTCGCCGAGCGCACCGGCAGCAGCGATa                           >  1:411616/1‑140 (MQ=255)                                                                                                  tggctggAGCGCAAACGCTCACCGCTCGATCCGGCAGTACGATCGCTTGTCAGCGCGCGCATTGCGCAAATGTGCCTGTGTGAGTTTTGTGTGGATATCACCAGTATGAAAGTCGCCGAGCGCACCGGCAGCAGCGATaa                          <  1:210929/140‑1 (MQ=255)                                                                                                      tggAGCGCAAACGCTCACCGCTCGATCCGGCAGTACGATCGCTTGTCAGCGCGCGCATTGCGCAAATGTGCCTGTGTGAGTTTTGTGTGGATATCACCAGTATGAAAGTCGCCGAGCGCACCGGCAGCAGCGATAAActg                      <  1:158970/140‑1 (MQ=255)                                                                                                          gcgcAAACGCTCACCGCTCGATCCGGCAGTACGATCGCTTGTCAGCGCGCGCATTGCGCAAATGTGCCTGTGTGAGTTTTGTGTGGATATCACCAGTATGAAAGTCGCCGAGCGCACCGGCAGCAGCGATAAACTGCTgg                  <  1:622975/140‑1 (MQ=255)                                                                                                                         gCTCGATCCGGCAGTACGATCGCTTGTCAGCGCGCGCATTGCGCAAATGTGCCTGTGTGAGTTTTGTGTGGATATCACCAGTATGAAAGTCGCCGAGCGCACCGGCAGCAGCGATAAACTGCTGGCAGTGGCTGACTggc   >  2:312500/1‑140 (MQ=255)                                                                                                                          cTCGATCCGGCAGTACGATCGCTTGTCAGCGCGCGCATTGCGCAAATGTGCCTGTGTGAGTTTTGTGTGGATATCACCAGTATGAAAGTCGCCGAGCGCACCGGCAGCAGCGATAAACTGCTGGCAGTGGCTGACTggcg  >  1:804013/1‑140 (MQ=255)                                                                                                                          cTCGATCCGGCAGTACGATCGCTTGTCAGCGCGCGCATTGCGCAAATGTGCCTGTGTGAGTTTTGAGTGGATATCACCAGTATGAAAGTCGCCGAGCGCACCGGCAGCAGCGATAAACTGCTGGCAGTGGCTGACTggcg  >  1:708849/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                  GTGGCTGCATCGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGGATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGGTAGTACGATCGCTTGTCAGCGCGCGCATTGCGCAAATGTGCCTGTGTGAGTTTTGTGTGGATATCACCAGTATGAAAGTCGCCGAGCGCACCGGCAGCAGCGATAAACTGCTGGCAGTGGCTGACTGGCG  >  NC_000913/1835565‑1835825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑