Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,860,490	T→C	K281K (AAA→AAG)	ydjL ←	putative zinc‑binding dehydrogenase YdjL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,860,490	0	T	C	100.0%	95.6 / NA	28	K281K (AAA→AAG)	ydjL	putative zinc‑binding dehydrogenase YdjL
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/12);  total (16/12)

CTTTGATAGCGCCGCTGGCTAATAGCCTGATAGCGTTACGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAATCACCAGTCCCAGATTGTCTTTGCCACAAATTTGCTGGC  >  NC_000913/1860369‑1860620                                                                                                                          |                                                                                                                                   cTTTGATAGCGCCGCTGGCTAATAGCCTGATAGCGTTACGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGCTTGAAGCCCATTCCAAcg                                                                                                                  >  2:121812/1‑140 (MQ=255)      aTAGCGCCGCTGGCTAATAGCCTGATAGCGTTACGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGCTTGAAGCCCATTCCAACGCGTAc                                                                                                             >  1:49346/1‑140 (MQ=255)          cgccgcTGGCTAATAGCCTGATAGCGTTACGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGCTTGAAGCCCATTCCAACGCGTACCACt                                                                                                         >  1:696149/1‑140 (MQ=255)          cgccgcTGGCTAATAGCCTGATAGCGTTACGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGCTTGAAGCCCATTCCAACGCGTACCACt                                                                                                         >  2:269483/1‑140 (MQ=255)            ccgcTGGCTAATAGCCTGATAGCGTTACGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGCTTGAAGCCCATTCCAACGCGTACCACTTc                                                                                                       <  1:749295/140‑1 (MQ=255)                       tAGCCTGATAGCGTTACGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGCTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGc                                                                                            >  2:130599/1‑140 (MQ=255)                        aGCCTGATAGCGTTACGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGCTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCg                                                                                           <  1:45068/140‑1 (MQ=255)                            tGATAGCGTTACGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGCTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGc                                                                                       >  2:804495/1‑140 (MQ=255)                            tGATAGCGTTACGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGCTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGc                                                                                       >  1:498011/1‑140 (MQ=255)                            tGATAGCGTTACGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGCTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGc                                                                                       >  1:743928/1‑140 (MQ=255)                                   gTTACGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGCTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGa                                                                                <  1:269483/140‑1 (MQ=255)                                    ttACGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGCTTGAAGCCCATTCCAACGCGTACCACTTCCCCATTCGGGCGGAGCATTTCGAt                                                                               >  1:143781/1‑140 (MQ=255)                                            tGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGCTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGtt                                                                       >  2:62398/1‑140 (MQ=255)                                                 tGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGCTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGt                                                                  <  2:743928/140‑1 (MQ=255)                                                 tGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGCTTGAAGCCCATCCCAACGCGTACCCCTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGt                                                                  >  2:152246/1‑140 (MQ=255)                                                   gAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGCTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGc                                                                <  2:204822/140‑1 (MQ=255)                                                   gAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGCTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGc                                                                <  2:49346/140‑1 (MQ=255)                                                          aGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGCTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTg                                                         >  1:263777/1‑140 (MQ=255)                                                             ccATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGCTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCa                                                      <  2:446489/140‑1 (MQ=255)                                                                    cccAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGCTTGAAGCCCATTCCAACGCGTACCACTTCCCCATTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGc                                               <  2:143781/140‑1 (MQ=255)                                                                            tgCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGCTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAATc                                       >  2:252314/1‑140 (MQ=255)                                                                            tgCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGCTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAATc                                       >  2:654847/1‑140 (MQ=255)                                                                              cgtgtGTTCCAGGCCGTAATGTCATTAATCGAGAAATCAAGAGGCTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAATCAc                                     <  2:498011/136‑1 (MQ=255)                                                                                 ttGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGCTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAATCACCAg                                  >  1:291234/1‑140 (MQ=255)                                                                                  tGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGCTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAATCACCAGt                                 <  2:400083/140‑1 (MQ=255)                                                                                   gTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGCTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAATCACCAGTc                                <  2:263777/140‑1 (MQ=255)                                                                                    ttCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGCTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAATCACCAGTcc                               <  2:291234/140‑1 (MQ=255)                                                                                         ggCGGTAATGTCATTAATCGAGAAATCAAGAGGCTTGAAGCCCATTCCAACGCGCACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAATCACCAGTCCCAGAt                          >  1:208918/1‑140 (MQ=255)                                                                                                                 aTCAAGAGGCTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAATCACCAGTCCCAGATTGTCTTTGCCACAAATTTGCTGGc  >  2:500309/1‑140 (MQ=255)                                                                                                                          |                                                                                                                                   CTTTGATAGCGCCGCTGGCTAATAGCCTGATAGCGTTACGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAATCACCAGTCCCAGATTGTCTTTGCCACAAATTTGCTGGC  >  NC_000913/1860369‑1860620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑