Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,882,947 G→A A346T (GCC→ACC)  dmlA → D‑malate/3‑isopropylmalate dehydrogenase (decarboxylating)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,882,9470GA100.0% 47.2 / NA 16A346T (GCC→ACC) dmlAD‑malate/3‑isopropylmalate dehydrogenase (decarboxylating)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/11);  total (5/11)

ATTTGGGCCGGGGCAATGATGCTCGATTTTCTCGGCAATGGCGATGAGCGTTTCCAGCAAGCGCATAACGGTATTCTGGCAGCAATTGAAGAAGTGATTGCTCACGGGCCGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTT  >  NC_000913/1882812‑1883082
                                                                                                                                       |                                                                                                                                       
aTTTGGGCCGGGGCAATGATGCTCGATTTTCTCGGCAATGGCGATGAGCGTTTCCAGCAAGCGCATAACGGTATTCTGGCAGCAATTGAAGAAGTGATTGCTCACGGGCCGAAAACACCTGATATGAAAGGCAATAccac                                                                                                                                     <  1:692785/140‑1 (MQ=255)
    gggCCGGGGCAATGATGCTCGATTTTCTCGGCAATGGCGATGAGCGTTTCCAGCAAGCGCATAACGGTATTCTGGCAGCAATTGAAGAAGTGATTGCTCACGGGCCGAAAACACCTGATATGAAAGGCAATACCACCACg                                                                                                                                 <  2:103468/140‑1 (MQ=255)
     ggCCGGGGCAATGATGCTCGATTTTCTCGGCAATGGCGATGAGCGTTTCCAGCAAGCGCATAACGGTATTCTGGCAGCAATTGAAGAAGTGATTGCTCACGGGCCGAAAACACCTGATATGAAAGGCAATACCACCACGc                                                                                                                                <  1:700500/140‑1 (MQ=255)
       ccGGGGCAATGATGCTCGATTTTCTCGGCAATGGCGATGAGCGTTTCCAGCAAGCGCATAACGGTATTCTGGCAGCAATTGAAGAAGTGATTGCTCACGGGCCGAAAACACCTGATATGAAAGGCAATACCACCACGCca                                                                                                                              <  1:44240/140‑1 (MQ=255)
                                    aaTGGCGATGAGCGTTTCCAGCAAGCGCATAACGGTATTCTGACAGCTATTGAAGAAGTGATTGCTCACGGGCAGAAAACACCTGATATGAAAGGCAATACCACCACGCCACAGGTTGCCGACGCGATTTGCAAAattat                                                                                                 >  2:488192/1‑140 (MQ=255)
                                            tGAGCGTTTCCAGCAAGCGCATAACGGTATTCTGGCAGCAATTGAAGAAGTGATTGCTCACGGGCCGAAAACACCTGATATGAAAGGCAATACCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGtt                                                                                         <  1:448890/140‑1 (MQ=255)
                                                    tCCAGCAAGCGCATAACGGTATTCTGGCAGCAATTGAAGAAGTGATTGCTCACGGGCCGAAAACACCTGATATGAAAGGCAATACCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCaa                                                                                 <  2:509292/140‑1 (MQ=255)
                                                      cAGCAAGCGCATAACGGTATTCTGGCAGCAATTGAAGAAGTGATTGCTCACGGGCCGAAAACACCTGATATGAAAGGCAATACCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAAc                                                                               <  2:173878/140‑1 (MQ=255)
                                                         cAAGCGCATAACGGTATTCTGGCAGCAATTGAAGAAGTGATTGCTCACGGGCCGAAAACACCTGATATGAAAGGCAATACCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAg                                                                            <  2:713017/140‑1 (MQ=255)
                                                             cgcATAACGGTATTCTGGCAGCAATTGAAGAAGTGATTGCTCACGGGCCGAAAACACCTGATATGAAAGGCAATACCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTa                                                                        >  1:123361/1‑140 (MQ=255)
                                                                   aCGGTATTCTGGCAGCAATTGAAGAAGTTATTCCTAACGCGCCGAAAACCCCTGATATGAAAGGCAATACCGCCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGCCAAACCAGTTTATTTGaa                                                                  <  2:391108/140‑1 (MQ=255)
                                                                                                        cGGGCCGAAAACACCTGATATGAAAGGCAATACCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGc                             <  1:132849/140‑1 (MQ=255)
                                                                                                        cGGGCCGAAAACACCTGATATGAAAGGCAATACCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGc                             <  2:123361/140‑1 (MQ=255)
                                                                                                                      cTGATATGAAAGGCAATACCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGc               >  2:656291/1‑140 (MQ=255)
                                                                                                                      cTGATATGAAAGGCAATACCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGc               >  1:296401/1‑140 (MQ=255)
                                                                                                                             gAAAGGCAATACCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCAttt        <  1:916/140‑1 (MQ=255)
                                                                                                                                aGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCAc     <  1:488192/140‑1 (MQ=255)
                                                                                                                                aGGCAATACCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCAc     >  2:719581/1‑140 (MQ=255)
                                                                                                                                   cAATACCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCtt  >  2:568393/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                       
ATTTGGGCCGGGGCAATGATGCTCGATTTTCTCGGCAATGGCGATGAGCGTTTCCAGCAAGCGCATAACGGTATTCTGGCAGCAATTGAAGAAGTGATTGCTCACGGGCCGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTT  >  NC_000913/1882812‑1883082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑