Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	130,624	G→A	P213S (CCC→TCC)	yacH ←	DUF3300 domain‑containing protein YacH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	130,624	0	G	A	100.0%	146.3 / NA	44	P213S (CCC→TCC)	yacH	DUF3300 domain‑containing protein YacH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/24);  total (20/24)

GTTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTGGCAGTTAAAACTGTATTGGATGGTATGACGGGTGCCGTGACTGTCTGTTTTACCGGTACAGCTTTCTTCGTTGTGGTAATAACTTTCTGTTCGGTTGATGACTTCAGCGAGCCGGTTTGTTGCGCCAGTTGCCGCAATCGCTGTACCGAGTCCATCACGTCCTGCGGC  >  NC_000913/130487‑130760                                                                                                                                          |                                                                                                                                         gTTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTGGCAGTTAAAACTGTATTGGATGGTATGACGGATg                                                                                                                                        >  1:586143/1‑140 (MQ=255) gTTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTGGCAGTTAAAACTGTATTGGATGGTATGACGGATg                                                                                                                                        >  1:305564/1‑140 (MQ=255) gTTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTGGCAGTTAAAACTGTATTGGATGGGATGACGGATg                                                                                                                                        >  2:460094/1‑140 (MQ=255)         aaaCCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTGGCAGTTAAAACTGTATTGGATGGTATGACGGATGCCGTGAct                                                                                                                                <  1:788620/140‑1 (MQ=255)                ggttgggttgTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGACGGGGGTGGCAGTTAAAACTGTATTGGATGGTATGACGGATGCCGTGACTGTCTGtt                                                                                                                         >  2:566813/1‑140 (MQ=255)                    gggttgTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAGTGGAAATGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTGGCAGTTAAAAGTGTATTGGATGGTATGACGGATGGCGGGACTGTCTGTTTTAc                                                                                                                     >  2:756985/1‑140 (MQ=255)                    gggttgTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTGGCAGTTAAAACTGTATTGGATGGTATGACGGATGCCGTGACTGTCTGTTTTAc                                                                                                                     <  1:756985/140‑1 (MQ=255)                     ggttgTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTGGCAGTTAAAACTGTATTGGATGGTATGACGGATGCCGTGACTGTCTGTTTTAcc                                                                                                                    <  1:416539/140‑1 (MQ=255)                     ggttgTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTGGCAGGTAAAACTGTATTGGGTGGTATGACGGGTGCCGGGTCTGTCTGTTTTagc                                                                                                                    >  2:641379/1‑138 (MQ=255)                         gTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTGGCAGTTAAAACTGTATTGGATGGTATGACGGATGCCGTGACTGTCTGTTTTACCGGTa                                                                                                                <  2:128431/140‑1 (MQ=255)                             ttGGTAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTGGCAGTTAAAACTGTATTGGATGGTATGACGGATGCCGTGACTGTCTGTTTTACCGGTACAGc                                                                                                            <  2:745513/140‑1 (MQ=255)                                    tataGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTGGCAGTTAAAACTGTATTGGATGGTATGACGGATGCCGTGACTGTCTGTTTTACCGGTACAGCTttc                                                                                                        >  2:252658/1‑137 (MQ=255)                                    tataGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTGGCAGTTAAAACTGTATTGGATGGTATGACGGATGCCGTGACTGTCTGTTTTACCGGTACAGCTttc                                                                                                        <  1:252658/137‑1 (MQ=255)                                     ataGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTGGCAGTTAAAACTGTATTGGATGGTATGACGGATGCCGTGACTGTCTGTTTTACCGGTACAGCTTTCTTCg                                                                                                    <  1:254906/140‑1 (MQ=255)                                       aGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTGGCAGTTAAAACTGTATTGGATGGTATGACGGATGCCGTGACTGTCTGTTTTACCGGTACAGCTTTCTTCGtt                                                                                                  >  1:540876/1‑140 (MQ=255)                                         aCCACATCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTGGCAGTTAAAACTGTATTGGATGGTATGACGGATGCCGTGACTGTCTGTTTTACCGGTACAGCTTTCTTCGTtgt                                                                                                >  2:756073/1‑140 (MQ=255)                                           cacaTCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTGGCAGTTAAAACTGTATTGGATGGTATGACGGATGCCGTGACTGTCTGTTTTACCGGTACAGCTTTCTTCGTTGTgg                                                                                              >  2:275251/1‑140 (MQ=255)                                           cacaTCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTGGCAGTTAAAACTGTATTGGATGGTATGACGGATGCCGTGACTGTCTGTTTTACCGGTACAGCTTTCTTCGTTGTgg                                                                                              <  2:135820/140‑1 (MQ=255)                                              aTCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTGGCAGTTAAAACTGTATTGGATGGTATGACGGATGCCGTGACTGTCTGTTTTACCGGTACAGCTTTCTTCGTtg                                                                                                 <  1:583642/134‑1 (MQ=255)                                              aTCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTGGCAGTTAAAACTGTATTGGATGGTATGACGGATGCCGTGACTGTCTGTTTTACCGGTACAGCTTTCTTCGTtg                                                                                                 >  2:583642/1‑134 (MQ=255)                                              aTCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTGGCAGTTAAAACTGTATTGGATGGTATGACGGATGCCGTGACTGTCTGTTTTACCGGTACAGCTTTCTTCGTTGTGGtaa                                                                                           <  1:756073/140‑1 (MQ=255)                                              aTCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTGGCAGTTAAAACTGTATTGGATGGTATGACGGATGCCGTGACTGTCTGTTTTACCGGTACAGCTTTCTTCGTTGTGGtaa                                                                                           <  1:275251/140‑1 (MQ=255)                                                cAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTGGCAGTTAAAACTGTATTGGATGGTATGACGGATGCCGTGACTGTCTGTTTTACCGGTACAGCTTTCTTCGTTGTGGtaata                                                                                         >  1:540188/1‑140 (MQ=255)                                                   gATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTGGCAGTTAAAACTGTATTGGATGGTATGACGGATGCCGTGACTGTCTGTTTTACCGGTACAGCTTTCTTCGTTGTGGTAATAACt                                                                                      <  2:259413/140‑1 (MQ=255)                                                     ttGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTGGCAGTTAAAACTGTATTGGATGGTATGACGGATGCCGTGACTGTCTGTTTTACCGGTACAGCTTTCTTCGTTGTGGTAATAACttt                                                                                    <  2:540876/140‑1 (MQ=255)                                                          gggCTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTGGCAGTTAAAACTGTATTGGATGGTATGACGGATGCCGTGACTGTCTGTTTTACCGGTACAGCTTTCTTCGTTGTGGTAATAACTTTCTGtt                                                                               <  2:540188/140‑1 (MQ=255)                                                          gggCTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTGGCAGTTAAAACTGTATTGGATGGTATGACGGATGCCGTGACTGTCTGTTTTACCGGTACAGCTTTCTTCGTTGTGGTAATAACTTTCTGtt                                                                               <  1:261126/140‑1 (MQ=255)                                                             cTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTGGCAGTTAAAACTGTATTGGATGGTATGACGGATGCCGTGACTGTCTGTTTTACCGGTACAGCTTTCTTCGTTGTGGTAATAACTTTCTGTTCgg                                                                            >  1:753668/1‑140 (MQ=255)                                                                      aaTGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTGGCAGTTAAAACTGTATTGGATGGTATGACGGATGCCGTGACTGTCTGTTTTACCGGTACAGCTTTCTTCGTTGTGGTAATAACTTTCTGTTCGGTTGATGACt                                                                   >  2:773181/1‑140 (MQ=255)                                                                        tGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTGGCAGTTAAAACTGTATTGGATGGTATGACGGATGCCGTGACTGTCTGTTTTACCGGTACAGCTTTCTTCGTTGTGGTAATAACTTTCTGTTCGGTTGATGACTTc                                                                 >  1:382092/1‑140 (MQ=255)                                                                        tGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTGGCAGTTAAAACTGTATTGGATGGTATGACGGATGCCGTGACTGTCTGTTTTACCGGTACAGCTTTCTTCGTTGTGGTAATAACTTTCTGTTCGGTTGATGACTTc                                                                 >  1:460921/1‑140 (MQ=255)                                                                         gACGGTTGTTGCCGGCTCTGTAATGACGGGGTTGGCAGTTAAAACTGTATTGGATGGTATGACGGATGCCGTGACTGTCTGTTTTACCGGTACAGCTTTCTTCGTTGTGGTAATAACTTTCTGTTCGGTTGATGACTTCa                                                                <  2:701365/140‑1 (MQ=255)                                                                            ggTTGTTGCCGGCTCTGTAATGACGGGGTTGGCAGTTAAAACTGTATTGGATGGTATGACGGATGCCGTGACTGTCTGTTTTACCGGTACAGCTTTCTTCGTTGTGGTAATAACTTTCTGTTCGGTTGATGACTTCAGCg                                                             <  2:305564/140‑1 (MQ=255)                                                                              ttgttgCCGGCTCTGTAATGACGGGGTTGGCAGTTAAAACTGTATTGGATGGTATGACGGATGCCGTGACTGTCTGTTTTACCGGTACAGCTTTCTTCGTTGTGGTAATAACTTTCTGTTCGGTTGATGACTTCAGCGAg                                                           <  1:566813/140‑1 (MQ=255)                                                                                        ctctGTAATGACGGGGTTGGCAGTTAAAACTGTATTGGATGGTATGACGGATGCCGTGACTGTCTGTTTTACCGGTACAGCTTTCTTCGTTGTGGTAATAACTTTCTGTTCGGTTGATGACTTCAGCGAGCCGGTttgtt                                                 >  1:249130/1‑140 (MQ=255)                                                                                                      ggTTGGCAGTTAAAACTGTATTGGATGGTATGACGGATGCCGTGACTGTCTGTTTTACCGGTACAGCTTTCTTCGTTGTGGTAATAACTTTCTGTTCGGTTGATGACTTCAGCGAGCCGGTTTGTTGCGCCAGTTGCCGc                                   <  2:785187/140‑1 (MQ=255)                                                                                                      ggTTGGCAGTTAAAACTGTATTGGATGGTATGACGGATGCCGTGACTGTCTGTTTTACCGGTACAGCTTTCTTCGTTGTGGTAATAACTTTCTGTTCGGTTGATGACTTCAGCGAGCCGGTTTGTTGCGCCAGTTGCCGc                                   <  1:641379/140‑1 (MQ=255)                                                                                                       gTTGGCAGTTAAAACTGTATTGGATGGTATGACGGATGCCGTGACTGTCTGTTTTACCGGTACAGCTTTCTTCGTTGTGGTAATAACTTTCTGTTCGGTTGATGACTTCAGCGAGCCGGTTTGTTGCGCCAGTTGCCGCa                                  >  2:587123/1‑140 (MQ=255)                                                                                                       gTTGGCAGTTAAAACTGTATTGGATGGTATGACGGATGCCGTGACTGTCTGTTTTACCGGTACAGCTTTCTTCGTTGTGGTAATAACTTTCTGTTCGGTTGATGACTTCAGCGAGCCGGTTTGTTGCGCCAGTTGCCGCa                                  >  2:354895/1‑140 (MQ=255)                                                                                                       gTTGGCAGTTAAAACTGTATTGGATGGTATGACGGATGCCGTGACTGTCTGTTTTACCGGTACAGCTTTCTTCGTTGTGGTAATAACTTTCTGTTCGGTTGATGACTTCAGCGAGCCGGTTTGTTGCGCCAGTTGCCGCa                                  >  2:119723/1‑140 (MQ=255)                                                                                                        ttGGCAGTTAAAACTGTATTGGATGGTATGACGGATGCCGTGACTGTCTGTTTTACCGGTACAGCTTTCTTCGTTGTGGTAATAACTTTCTGTTCGGTTGATGACTTCAGCGAGCCGGTTTGTTGCGCCAGTTGCCGCaa                                 <  1:354895/140‑1 (MQ=255)                                                                                                                 aaaaCTGTATTGGATGGTATGACGGATGCCGTGACTGTCTGTTTTACCGGTACAGCTTTCTTCGTTGTGGTAATAACTTTCTGTTCGGTTGATGACTTCAGCGAGCCGGTTTGTTGCGCCAGTTGCCGCAATCGCTGTAc                        >  2:412532/1‑140 (MQ=255)                                                                                                                  aaaCTGTATTGGATGGTATGACGGATGCCGTGACTGTCTGTTTTACCGGTACAGCTTTCTTCGTTGTGGTAATAACTTTCTGTTCGGTTGATGACTTCAGCGAGCCGGTTTGTTGCGCCAGTTGCCGCAATCGCTGTAcc                       >  2:593239/1‑140 (MQ=255)                                                                                                                   aaCTGTATTGGATGGTATGACGGATGCCGTGACTGTCTGTTTTACCGGTACAGCTTTCTTCGTTGTGGTAATAACTTTCTGTTCGGTTGATGACTTCAGCGAGCCGGTTTGTTGCGCCAGTTGCCGCAATCGCTGTACCg                      <  1:460094/140‑1 (MQ=255)                                                                                                                                  tATGACGGATGCCGTGACTGTCTGTTTTACCGGTACAGCTTTCTTCGTTGTGGTAATAACTTTCTGTTCGGTTGATGACTTCAGCGAGCCGGTTTGTTGCGCCAGTTGCCGCAATCGCTGTACCGAGTCCATCACg           <  2:539522/136‑1 (MQ=255)                                                                                                                                  tATGACGGATGCCGTGACTGTCTGTTTTACCGGTACAGCTTTCTTCGTTGTGGTAATAACTTTCTGTTCGGTTGATGACTTCAGCGAGCCGGTTTGTTGCGCCAGTTGCCGCAATCGCTGTACCGAGTCCATCACg           >  1:539522/1‑136 (MQ=255)                                                                                                                                  tATGACGGATGCCGTGACTGTCTGTTTTACCGGTACAGCTTTCTTCGTTGTGGTAATAACTTTCTGTTCGGTTGATGACTTCAGCGAGCCGGTTTGTTGCGCCAGTTGCCGCAATCGCTGTACCGAGTCCATCACGTCCt       >  2:330729/1‑140 (MQ=255)                                                                                                                                       cGGATGCCGTGACTGTCTGTTTTACCGGTACAGCTTTCTTCGTTGTGGTAATAACTTTCTGTTCGGTTGATGACTTCAGCGAGCCGGTTTGTTGCGCCAGTTGCCGCAATCGCTGTACCGAGTCCATCACGTCCTGCGGc  <  2:460921/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         GTTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTGGCAGTTAAAACTGTATTGGATGGTATGACGGGTGCCGTGACTGTCTGTTTTACCGGTACAGCTTTCTTCGTTGTGGTAATAACTTTCTGTTCGGTTGATGACTTCAGCGAGCCGGTTTGTTGCGCCAGTTGCCGCAATCGCTGTACCGAGTCCATCACGTCCTGCGGC  >  NC_000913/130487‑130760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑