Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,997,792 T→C N8S (AAC→AGC)  tcyN ← cystine ABC transporter ATP binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,997,7920TC100.0% 137.3 / NA 39N8S (AAC→AGC) tcyNcystine ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/24);  total (15/24)

TTCCAGCAGATTTATGCTGCGTAGCAACGTGGTTTTGCCGGAACCACTCGGACCGATAATTGCCACCACTTCGCCAGGCTTTACCTCAAGGTCGATACCGTGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGTTCTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGATTCTCAAAATGGTTCTGCAACGTCGATAACACAGTCGCCATGATCCAGTAGATGAGCGAAGCCGCCAGATACA  >  NC_000913/1997656‑1997917
                                                                                                                                        |                                                                                                                             
ttCCAGCAGATTTATGCTGCGTAGCAACGTGGTTTTGCCGGAACCACTCGGACCGATAATTGCCACCACTTCGCCAGGCTTTACCTCAAGGTCGATACCGTGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGctct                                                                                                                            >  2:697176/1‑140 (MQ=255)
         aTTTATGCTGCGTAGCAACGTGGTTTTGCCGGAACCTCTCGGACCGATAATTGCCACCACTTCGCCAGGCTTTACCTCAAGGTCGATACCGTGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGCTCTTAACTTCaa                                                                                                                   >  1:167917/1‑140 (MQ=255)
         aTTTATGCTGCGTAGCAACGTGGTTTTGCCGGAACCACTCGGACCGATAATTGCCACCACTTCGCCAGGCTTTACCTCAAGGTCGATACCGTGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGCTCTTAACTTCaa                                                                                                                   <  2:121876/140‑1 (MQ=255)
         aTTTATGCTGCGTAGCAACGTGGTTTTGCCGGAACCACTCGGACCGATAATTGCCACCACTTCGCCAGGCTTTACCTCAAGGTCGATACCGTGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGCTCTTAACTTCaa                                                                                                                   <  1:697176/140‑1 (MQ=255)
                   cGTAGCAACGTGGTTTTGCCGGAACCACTCGGACCGATAATTGCCACCACTTCGCCAGGCTTTACCTCAAGGTCGATACCGTGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGCTCTTAACtt                                                                                                                      <  1:115085/127‑1 (MQ=255)
                   cGTAGCAACGTGGTTTTGCCGGAACCACTCGGACCGATAATTGCCACCACTTCGCCAGGCTTTACCTCAAGGTCGATACCGTGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGCTCTTAACtt                                                                                                                      >  2:115085/1‑127 (MQ=255)
                   cGTAGCAACGTGGTTTTGCCGGAACCACTCGGACCGATAATTGCCACCACTTCGCCAGGCTTTACCTCAAGGTCGATACCGTGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGCTCTTAACTTCAATGGCACTCAt                                                                                                         >  1:610210/1‑140 (MQ=255)
                     tAGCAACGTGGTTTTGCCGGAACCACTCGGACCGATAATTGCCACCACTTCGCCAGGCTTTACCTCAAGGTCGATACCGTGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGCTCTTAACTTCAATGGCACTCAttt                                                                                                       >  1:466238/1‑140 (MQ=255)
                          aCGTGGTTTTGCCGGAACCACTCGGACCGATAATTGCCACCACTTCGCCAGGCTTTACCTCAAGGTCGATACCGTGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGCTCTTAACTTCAATGGCACTCATTTTGGtt                                                                                                  <  2:136630/140‑1 (MQ=255)
                            gTGGTTTTGCCGGAACCACTCGGACCGATAATTGCCACCACTTCGCCAGGCTTTACCTCAAGGTCGATACCGTGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGCTCTTAACTTCAATGGCACTCATTTTGGTtct                                                                                                <  1:233469/140‑1 (MQ=255)
                                ttttGCCGGAACCACTCGGACCGATAATTGCCACCACTTCGCCAGGCTTTACCTCAAGGTCGATACCGTGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGCTCTTAACTTCAATGGCACTCATTTTGGTtctctct                                                                                            >  1:305995/1‑140 (MQ=255)
                                              cTCGGACCGATAATTGCCACCACTTCGCCAGGCTTTACCTCAAGGGCGATACCGTGCAGCACCGTCTGACCGTGGTATTTTTTCACCAGGTTCTTAACTTCAATGGCAATCATTTTGGCTCTCTCTCCTGTCGAATACGt                                                                              >  2:99328/1‑140 (MQ=255)
                                                   aCCGATAATTGCCACCACTTCGCCAGGCTTTACCTCAAGGTCGATACCGTGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGCTCTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGAtt                                                                         <  2:526238/140‑1 (MQ=255)
                                                   aCCGATAATTGCCACCACTTCGCCAGGCTTTACCTCAAGGTCGATACCGTGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGCTCTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGAtt                                                                         <  2:305995/140‑1 (MQ=255)
                                                          aTTGCCACCACTTCGCCAGGCTTTACCTCAAGGTCGATACCGTGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGCTCTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGATTCTCaaaa                                                                  <  2:19066/140‑1 (MQ=255)
                                                               caccacTTCGCCAGGCTTTACCTCAAGGTCGATACCGTGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGCTCTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGATTCTCAAAATGGtt                                                             >  1:39449/1‑140 (MQ=255)
                                                                  cacTTCGCCAGGCTTTACCTCAAGGTCGATACCGTGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGCTCTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGATTCTCAAAATGGTTCTg                                                          >  2:353811/1‑140 (MQ=255)
                                                                  cacTTCGCCAGGCTTTACCTCAAGGTCGATACCGTGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGCTCTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGATTCTCAAAATGGTTCTg                                                          >  2:298712/1‑140 (MQ=255)
                                                                    cTTCGCCAGGCTTTACCTCAAGGTCGATACCGTGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGCTCTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGATTCTCAAAATGGTTCTGCa                                                        <  2:485727/140‑1 (MQ=255)
                                                                    cTTCGCCAGGCTTTACCTCAAGGTCGATACCGTGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGCTCTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGATTCTCAAAATGGTTCTGCa                                                        <  1:735797/140‑1 (MQ=255)
                                                                      tCGCCAGGCTTTACCTCAAGGTCGATACCGTGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGCTCTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGATTCTCAAAATGGTTCTGCAAc                                                      <  2:209815/140‑1 (MQ=255)
                                                                                    cTCAAGGTCGATACCGTGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGCTCTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGATTCTCAAAATGGTTCTGCAACGTCGATAACACAGt                                        <  1:19186/140‑1 (MQ=255)
                                                                                       aaGGTCGATACCGTGCAGCTCCGTCTGACCGTGGAATTTTTTCACCAGGCTCTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGATTCTCAAAATGGTTCTGCAACGTCGATAACACAGTCGc                                     <  1:247157/140‑1 (MQ=255)
                                                                                       aaGGTCGATACCGTGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGCTCTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGATTCTCAAAATGGTTCTGCAACGTCGATAACACAGTCGc                                     <  2:167917/140‑1 (MQ=255)
                                                                                         ggTCGATACCGTGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGCTCTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGATTCTCAAAATGGTTCTGCAACGTCGATAACACAGTCGCCa                                   <  1:626723/140‑1 (MQ=255)
                                                                                         ggTCGATACCGTGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGCTCTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGATTCTCAAAATGGTTCTGCAACGTCGATAACACAGTCGCCa                                   <  2:308901/140‑1 (MQ=255)
                                                                                         ggTCGATACCGTGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGCTCTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGATTCTCAAAATGGTTCTGCAACGTCGATAACACAGTCGCCa                                   <  1:562429/140‑1 (MQ=255)
                                                                                                    tGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGCTCTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGATTCTCAAAATGGTTCTGCAACGTCGATAACACAGTCGcc                                    >  2:765636/1‑128 (MQ=255)
                                                                                                    tGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGCTCTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGATTCTCAAAATGGTTCTGCAACGTCGATAACACAGTCGcc                                    <  1:765636/128‑1 (MQ=255)
                                                                                                    tGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGCTCTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGATTCTCAAAATGGTTCTGCAACGTCGATAACACAGTCGCCATGATCCAGTAg                        >  1:110759/1‑140 (MQ=255)
                                                                                                    tGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGCTCTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGATTCTCAAAATGGTTCTGCAACGTCGATAACACAGTCGCCATGATCCAGTAg                        >  2:36212/1‑140 (MQ=255)
                                                                                                     gcagcaCCGTCTGACCGTGGAATTTTTTCACCAGGCTCTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGATTCTCAAAATGGTTCTGCAACGTCGATAACACAGTCGCCa                                   >  2:18670/1‑128 (MQ=255)
                                                                                                     gcagcaCCGTCTGACCGTGGAATTTTTTCACCAGGCTCTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGATTCTCAAAATGGTTCTGCAACGTCGATAACACAGTCGCCa                                   <  1:18670/128‑1 (MQ=255)
                                                                                                         caCCGTCTGACCGTGGAATTTTTTCACCAGGCTCTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGATTCTCAAAATGGTTCTGCAACGTCGATAACACAGTCGCCa                                   >  1:280312/1‑124 (MQ=255)
                                                                                                         caCCGTCTGACCGTGGAATTTTTTCACCAGGCTCTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGATTCTCAAAATGGTTCTGCAACGTCGATAACACAGTCGCCa                                   <  2:280312/124‑1 (MQ=255)
                                                                                                         caCCGTCTGACCGTGGAATTTTTTCACCAGGCTCTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGATTCTCAAAATGGTTCTGCAACGTCGATAACACAGTCGCCATGATCCAGTAGATGAg                   >  2:184048/1‑140 (MQ=255)
                                                                                                            cGTCTGACCGTGGAATTTTTTCACCAGGCTCTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGATTCTCAAAATGGTTCTGCAACGTCGATAACACAGTCGCCATGATCCAGTAGATGAGCGa                <  1:298712/140‑1 (MQ=255)
                                                                                                              tCTGACCGTGGAATTTTTTCACCAGGCTCTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGATTCTCAAAATGGTTCTGCAACGTCGATAACACAGTCGCCATGATCCAGTAGATGAGCGAAg              >  2:561743/1‑140 (MQ=255)
                                                                                                                    cGTGGAATTTTTTCACCAGGCTCTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGATTCTCAAAATGGTTCTGCAACGTCGATAACACAGTCGCCATGATCCAGTAGATGAGCGAAGCCGCCa        <  2:414640/140‑1 (MQ=255)
                                                                                                                         aaTTTTTTCACCAGGCTCTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGATTCTCAAAATGGTTCTGCAACGTCGATAACACAGTCGCCATGATCCAGTAGATGAGCGAAGCCGCCAGATAc   <  1:184048/140‑1 (MQ=255)
                                                                                                                          aTTTTTTCACCAGGCTCTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGATTCTCAAAATGGTTCTGCAACGTCGATAACACAGTCGCCATGATCCAGTAGATGAGCGAAGCCGCCAGATACa  <  1:36212/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                             
TTCCAGCAGATTTATGCTGCGTAGCAACGTGGTTTTGCCGGAACCACTCGGACCGATAATTGCCACCACTTCGCCAGGCTTTACCTCAAGGTCGATACCGTGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGTTCTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGATTCTCAAAATGGTTCTGCAACGTCGATAACACAGTCGCCATGATCCAGTAGATGAGCGAAGCCGCCAGATACA  >  NC_000913/1997656‑1997917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑