Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,106,559	A→G	V223A (GTT→GCT)	wbbH ←	putative O‑antigen polymerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,106,559	0	A	G	100.0%	224.7 / NA	62	V223A (GTT→GCT)	wbbH	putative O‑antigen polymerase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/28);  total (34/28)

TGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAA  >  NC_000913/2106428‑2106692                                                                                                                                    |                                                                                                                                      tGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAGCACCTACa                                                                                                                               >  1:744324/1‑140 (MQ=255)  ggCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAGCACCTACAg                                                                                                                              <  1:420204/140‑1 (MQ=255)       agTTAGATACTTGCTGAAAATAAAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAGCACCTACAGCTGta                                                                                                                         <  1:456208/140‑1 (MQ=255)                 ttGCTGAAAATAAAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAGCACCTACAGCTGTAATAAGATaaa                                                                                                               >  2:170715/1‑140 (MQ=255)                  tGCTGAAAATAAAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAGCACCTACAGCTGTAATAAGATAAAc                                                                                                              >  1:375127/1‑140 (MQ=255)                   gCTGAAAATAAAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAGCACCTACAGCTGTAATAAGATAAACa                                                                                                             >  1:568919/1‑140 (MQ=255)                   gCTGAAAATAAAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAGCACCTACAGCTGTAATAAGATAAACa                                                                                                             >  2:754594/1‑140 (MQ=255)                     tGAAAATAAAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAGCACCTACAGCTGTAATAAGATAAACATa                                                                                                           <  1:545150/140‑1 (MQ=255)                     tGAAAATAAAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAGCACCTACAGCTGTAATAAGATAAACATa                                                                                                           <  2:378140/140‑1 (MQ=255)                       aaaaTAAAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAGCACCTACAGCTGTAATAAGATAAACATAAt                                                                                                         >  2:94327/1‑140 (MQ=255)                              aaCTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTAc                                                                                                  <  1:650629/140‑1 (MQ=255)                                           cgATTATAGGGCTGACCAAATACATGGATAGATAATATGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCt                                                                                     <  1:561765/140‑1 (MQ=255)                                                ataGGGCTGACCAAATACATGGATAGATAATATGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTTCGAt                                                                                <  1:94327/140‑1 (MQ=255)                                                ataGGGCTGACCAAATACATGGATAGATAATATGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGAt                                                                                <  2:375127/140‑1 (MQ=255)                                                ataGGGCTGACCAAATACATGGATAGATAATATGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGAt                                                                                <  2:261730/140‑1 (MQ=255)                                                ataGGGCTGACCAAATACATGGATAGATAATATGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGAt                                                                                <  2:547/140‑1 (MQ=255)                                                  aGGGCTGACCAAATACATGGATAGATAATATGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGa                                                                              <  2:744324/140‑1 (MQ=255)                                                    ggCTGACCAAATACATGGATAGATAATATGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAAt                                                                            >  1:189524/1‑140 (MQ=255)                                                       tGACCAAATACATGGATAGATAATATGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCa                                                                         <  1:170715/140‑1 (MQ=255)                                                        gACCAAATACATGGATAGATAATATGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCAt                                                                        >  1:251554/1‑140 (MQ=255)                                                        gACCAAATACATGGATAGATAATATGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCAt                                                                        >  1:241361/1‑140 (MQ=255)                                                         aCCAAATACATGGATAGATAATATGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATa                                                                       >  1:428318/1‑140 (MQ=255)                                                             aaTACATGGATAGATAATATGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAagag                                                                   <  2:241361/140‑1 (MQ=255)                                                              aTACATGGATAGATAATATGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAAgaga                                                                  >  1:223824/1‑140 (MQ=255)                                                                   tGGATAGATAATATGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAAGAGATGATa                                                             >  1:659387/1‑140 (MQ=255)                                                                         gataATATGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATa                                                       >  1:551909/1‑140 (MQ=255)                                                                         gataATATGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATa                                                       >  2:312519/1‑140 (MQ=255)                                                                               atGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACa                                                 >  1:155027/1‑140 (MQ=255)                                                                                tGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACaa                                                >  1:137214/1‑140 (MQ=255)                                                                                 gCCATCCCCCCAGCCAGTCCACGTAAAAAGAGCATATACATGGCGAATAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAAt                                               >  2:226922/1‑140 (MQ=255)                                                                                    aTCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACATGGAGAATAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTg                                            >  2:798795/1‑140 (MQ=255)                                                                                    aTCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAAGAgatgat                                                              >  2:221678/1‑122 (MQ=255)                                                                                    aTCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAAGAgatgat                                                              <  1:221678/122‑1 (MQ=255)                                                                                    aTCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTg                                            <  2:189524/140‑1 (MQ=255)                                                                                    aTCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTg                                            >  2:49303/1‑140 (MQ=255)                                                                                      ccccccAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACGCCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCt                                          <  2:760764/140‑1 (MQ=255)                                                                                      ccccccAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCt                                          <  1:277942/140‑1 (MQ=255)                                                                                           cAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCa                                     <  1:754594/140‑1 (MQ=255)                                                                                           cAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCa                                     <  2:568919/140‑1 (MQ=255)                                                                                              gCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTa                                  <  2:296095/140‑1 (MQ=255)                                                                                              gCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTa                                  <  1:532157/140‑1 (MQ=255)                                                                                                aGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTAtt                                <  1:282005/140‑1 (MQ=255)                                                                                                aGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTAtt                                <  2:251554/140‑1 (MQ=255)                                                                                                  tCCACGTAAAAAGAGCATATACAAGGAGAATAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCa                              >  1:282115/1‑140 (MQ=255)                                                                                                  tCCACGTAAAAAGAGCATATACAAGGAGAATAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCa                              >  2:319481/1‑140 (MQ=255)                                                                                                  tCCACGTAAAAAGAGCATATACAAGGAGAATAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCa                              >  1:245077/1‑140 (MQ=255)                                                                                                  tCCACGTAAAAAGAGCATATACAAGGAGAATAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCa                              >  1:537277/1‑140 (MQ=255)                                                                                                             agagCATATACAAGGAGAATAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCa                   <  2:245077/140‑1 (MQ=255)                                                                                                             agagCATATACAAGGAGAATAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCa                   <  2:223824/140‑1 (MQ=255)                                                                                                                 cATATACAAGGAGAATGGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGa               >  2:289476/1‑140 (MQ=255)                                                                                                                 cATATACAAGGAGAATAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGa               >  2:431192/1‑140 (MQ=255)                                                                                                                 cATATACAAGGAGAATAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGa               >  1:6059/1‑140 (MQ=255)                                                                                                                      aCAAGGAGAATAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAAt          <  2:537277/140‑1 (MQ=255)                                                                                                                      aCAAGGAGAATAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAAt          <  1:312519/140‑1 (MQ=255)                                                                                                                      aCAAGGAGAATAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAAt          >  2:190828/1‑140 (MQ=255)                                                                                                                      aCAAGGAGAATAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAAt          <  1:49303/140‑1 (MQ=255)                                                                                                                         aGGAGAATAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAACTGCAAAGATGAATACa       >  1:300799/1‑140 (MQ=255)                                                                                                                          ggAGAATAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACaa      >  1:421689/1‑140 (MQ=255)                                                                                                                           gagaATAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAAt     >  1:600712/1‑140 (MQ=255)                                                                                                                            agaATAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATa    >  2:749582/1‑140 (MQ=255)                                                                                                                              aaTAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATaaa  >  1:376403/1‑140 (MQ=255)                                                                                                                              aaTAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATaaa  >  2:590604/1‑140 (MQ=255)                                                                                                                              aaTAGAGCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATaaa  >  1:698705/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                      TGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAA  >  NC_000913/2106428‑2106692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑