Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,125,891	A→G	V111A (GTA→GCA)	gmm ←	GDP‑mannose mannosyl hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,125,891	0	A	G	100.0%	93.0 / NA	27	V111A (GTA→GCA)	gmm	GDP‑mannose mannosyl hydrolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/16);  total (11/16)

CTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAGTTATCGTCATAAAAGTGCTGCCAGACACCGTAAAACTGGCCTGCTGTTATCGGCAAACGCAGCCCC  >  NC_000913/2125757‑2126013                                                                                                                                       |                                                                                                                           cTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATGCTCTg                                                                                                                       >  1:367492/1‑140 (MQ=255)    cGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATGCTCTGAAg                                                                                                                    >  1:378175/1‑140 (MQ=255)            ttCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATGCTCTGAAGCGAAAAcc                                                                                                            >  1:371544/1‑140 (MQ=255)            ttCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATGCTCTGAAGCGAAAAcc                                                                                                            >  2:631191/1‑140 (MQ=255)            ttCTCAGCGAGAAAATAGGCGCGGCTCTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATGCTCTGAAGCGAAAAcc                                                                                                            <  1:566407/140‑1 (MQ=255)              ctcAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATGCTCTGAAGCGAAAACCGa                                                                                                          <  2:711380/140‑1 (MQ=255)              ctcAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATGCTCTGAAGCGAAAACCGa                                                                                                          <  2:646506/140‑1 (MQ=255)               tcAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATGCTCTGAAGCGAAAACCGAg                                                                                                         <  2:683949/140‑1 (MQ=255)                  gCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATGCTCTGAAGCGAAAACCGAGcac                                                                                                      <  1:263104/140‑1 (MQ=255)                  gCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATGCTCTGAAGCGAAAACCGAGcac                                                                                                      <  2:68984/140‑1 (MQ=255)                                      gTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATGCTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAAt                                                                                  <  2:86334/140‑1 (MQ=255)                                         aGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATGCTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCg                                                                               <  1:783435/140‑1 (MQ=255)                                            aTGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATGCTCTGAAGCGAAAACCGAGCACCACATagtgag                                                                                           >  2:437425/1‑125 (MQ=255)                                            aTGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATGCTCTGAAGCGAAAACCGAGCACCACATagtgag                                                                                           <  1:437425/125‑1 (MQ=255)                                                     aTCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATGCTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAGt                                                                   >  1:504429/1‑140 (MQ=255)                                                        aCTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATGCTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAGTTAt                                                                <  1:631191/140‑1 (MQ=255)                                                          tGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATGCTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAGTTATCg                                                              >  1:674276/1‑140 (MQ=255)                                                            gCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATGCTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAGTTATCGTc                                                            >  2:579282/1‑140 (MQ=255)                                                               agcagcGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATGCTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAGTTATCGTCATa                                                         >  2:737982/1‑140 (MQ=255)                                                                   gcgcGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATGCTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAGTTATCGTCATAAAAg                                                     <  1:350702/140‑1 (MQ=255)                                                                   gcgcGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATGCTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAGTTATCGTCATAAAAg                                                     <  2:803924/140‑1 (MQ=255)                                                                             cGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATGCTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAGTTATCGTCATAAAAGTGCTGCCAGa                                           <  1:737982/140‑1 (MQ=255)                                                                             cGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATGCTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAGTTATCGTCATAAAAGTGCTGCCAGa                                           <  2:504429/140‑1 (MQ=255)                                                                                            aTCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATGCTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAGTTATCGTCATAAAAGTGCTGCCAGACACCGTAAAACTGGc                            <  2:674276/140‑1 (MQ=255)                                                                                               gTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATGCTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAGTTATCGTCATAAAAGTGCTGCCAGACACCGTAAAACTGGCctg                         <  1:579282/140‑1 (MQ=255)                                                                                                                  aGTAACAGCTCTTCTTCCGATGCTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAGTTATCGTCATAAAAGTGCTGCCAGACACCGTAAAACTGGCCTGCTGTTATCGGCAAACGCAg      >  1:253127/1‑140 (MQ=255)                                                                                                                      aCAGCTCTTCTTCCGATGCTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAGTTATCGTCATAAAAGTGCTGCCAGACACCGTAAAACTGGCCTGCTGTTATCGGCAAACGCAGcccc  >  1:272863/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                           CTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAGTTATCGTCATAAAAGTGCTGCCAGACACCGTAAAACTGGCCTGCTGTTATCGGCAAACGCAGCCCC  >  NC_000913/2125757‑2126013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑