Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,156,246	G→A	V328V (GTG→GTA)	mdtB →	multidrug efflux pump RND permease subunit MdtB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,156,246	0	G	A	100.0%	132.2 / NA	39	V328V (GTG→GTA)	mdtB	multidrug efflux pump RND permease subunit MdtB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/22);  total (17/22)

TGGGCGAACAAAGAACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCACCACCAATATCCGCGCATCCGTCGATGATACTCAGTTTGAATTGATGATGGCTATCGCGCTGGTAGTCATGATTATCTACCTGTTTTTGCGCAATATTCCGGCGACCATCATT  >  NC_000913/2156109‑2156372                                                                                                                                          |                                                                                                                               tGGGCGAACAAAGAACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTACt                                                                                                                              >  2:372581/1‑140 (MQ=255)       aaCAAAGAACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTATCATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTACTTTCCGa                                                                                                                        >  1:243820/1‑140 (MQ=255)        aCAAAGAACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTACTTTCCGAt                                                                                                                       <  1:26133/140‑1 (MQ=255)        aCAAAGAACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTACTTTCCGAt                                                                                                                       <  1:372581/140‑1 (MQ=255)                    ccATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTACTTTCCGATCGCACCACCAat                                                                                                           <  2:530865/140‑1 (MQ=255)                        tgtgATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTACTTTCCGATCGCACCACCAATATcc                                                                                                       >  2:720045/1‑140 (MQ=255)                        tgtgATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTACTTTCCGATCGCACCACCAATATcc                                                                                                       >  1:186817/1‑140 (MQ=255)                        tgtgATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTACTTTCCGATCGCACCACCAATATcc                                                                                                       >  2:391650/1‑140 (MQ=255)                           gatgaATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTACTTTCCGATCGCACCACCAATATCcgcg                                                                                                    <  2:186817/140‑1 (MQ=255)                              gaATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTACTTTCCGATCGCACCACCAATATCCGCGCAt                                                                                                 <  2:486037/140‑1 (MQ=255)                              gaATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTACTTTCCGATCGCACCACCAATATCCGCGCAt                                                                                                 <  1:255496/140‑1 (MQ=255)                                           cAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTACTTTCCGATCGCACCACCAATATCcgcg                                                                                                    >  2:619012/1‑124 (MQ=255)                                           cAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTACTTTCCGATCGCACCACCAATATCcgcg                                                                                                    <  1:619012/124‑1 (MQ=255)                                             gCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTACTTTCCGATCGCACCACCAATATCCGCGCATCCGTCGATGATACTc                                                                                  <  2:806481/140‑1 (MQ=255)                                                         cATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTACTTTCCGATCGCACCACCAATATCCGCGCATCCGTCGATGATACTCAGTTTGAATtga                                                                      <  1:365841/140‑1 (MQ=255)                                                               ctcCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTACTTTCCGATCGCACCACCAATATCCGCGCATCCGTCGATGATACTCAGTTTGAATTGATGATgg                                                                >  1:242283/1‑140 (MQ=255)                                                                   aCCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTACTTTCCGATCGCACCACCAATATCCGCGCATCCGTCGATGATACTCAGTTTGAATTGATGATGGCTAt                                                            <  1:197832/140‑1 (MQ=255)                                                                    ccgccgACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTACTTTCCGATCGCACCACCAATATCCGCGCATCCGTCGATGATACTCAGTTTGAATTGATGATGGCTATc                                                           >  2:490337/1‑140 (MQ=255)                                                                     cgccgACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTACTTTCCGATCGCACCACCAATATCCGCGCATCCGTCGATGATACTCAGTTTGAATTGATGATGGCTATcg                                                          <  1:391650/140‑1 (MQ=255)                                                                      gccgACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTACTTTCCGATCGCACCACCAATATCCGCGCATCCGTCGATGATACTCTGTTTGAATTGATGATGGCTATcgc                                                         <  2:383370/140‑1 (MQ=255)                                                                        cgACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTACTTTCCGATCGCACCACCAATATCCGCGCATCCGTCGATGATACTCAGTTTGAATTGATGATGGCTATCgcgc                                                       <  2:242283/140‑1 (MQ=255)                                                                          aCAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTACTTTCCGATCGCACCACCAATATCCGCGCATCCGTCGATGATACTCAGTTTGAATTGATGATGGCTATCGCGCTg                                                     <  1:247419/140‑1 (MQ=255)                                                                          aCAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTACTTTCCGATCGCACCACCAATATCCGCGCATCCGTCGATGATACTCAGTTTGAATTGATGATGGCTATCGCGCTg                                                     <  2:243820/140‑1 (MQ=255)                                                                          aCAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTACCTTCCGATCGCACCACCAATATCCGCGCATCCGTCGATGATACTCAGTTTGAATTGATGATGGCTATCGCGCTg                                                     >  2:263593/1‑140 (MQ=255)                                                                           cAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTACTTTCCGATCGCACCACCAATATCCGCGCATCCGTCGATGATACTCAGTTTGAATTGATGATGGCTATCGCGCTgg                                                    <  1:490337/140‑1 (MQ=255)                                                                                             gcCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTACTTTCCGATCGCACCACCAATATCCGCGCATCCGTCGATGATGCTCAGTTTGAATTGATGATGGCTATCGCGCTGGTAGTCAt                                             <  2:296108/129‑1 (MQ=255)                                                                                             gcCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTACTTTCCGATCGCACCACCAATATCCGCGCATCCGTCGATGATACTCAGTTTGAATTGATGATGGCTATCGCGCTGGTAGTCAt                                             >  1:296108/1‑129 (MQ=255)                                                                                                  aGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTACCTTCCGATCGCACCACCAATATCCGCGCATCCGTCGATGATACTCAGTTTGAATTGATGATGGCTATCGCGCTGGTAGTCATGATTATCTACCTGttt                             <  1:263593/140‑1 (MQ=255)                                                                                                   gCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTACTTTCCGATCGCACCACCAATATCCGCGCATCCGTCGATGATACTCAGTTTGAATTGATGATGGCTATCGCGCTGGTAGTCATGATTATCTACCTGtttt                            >  1:266097/1‑140 (MQ=255)                                                                                                       aCTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTACTTTCCGATCGCACCACCAATATCCGCGCATCCGTCGATGATACTCAGTTTGAATTGATGATGGCTATCGCGCTGGTAGTCATGATTATCTACCTGTTTTTgcg                        <  2:218837/140‑1 (MQ=255)                                                                                                              gTCTGCCGCAATCGGTGAAGGTGACAGTACTTTCCGATCGCACCACCAATATCCGCGCATCCGTCGATGATACTCAGTTTGAATTGATGATGGCTATCGCGCTGGTAGTCATGATTATCTACCTGTTTTTGCGCAATAtt                 >  2:744333/1‑140 (MQ=255)                                                                                                               tCTGCCGAAATCGGTGAAGGTGACAGTACTTTCCGATCGCACCACCAATATCCGCGCATCCGTCGATGATACTCAGTTTGAATTGATGATGGCTATCGCGCTGGTAGTCATGATTATCTACCTGTTTTTGCGCAATATTc                >  1:322551/1‑140 (MQ=255)                                                                                                                 tGCCGAAATCGGTGAAGGTGACAGTACTTTCCGATCGCACCACCAATATCCGCGCATCCGTCGATGATACTCAGTTTGAATTGATGATGGCTATCGCGCTGGTAGTCATGATTATCTACCTGTTTTTGCGCAATATTCCg              >  1:266876/1‑140 (MQ=255)                                                                                                                 tGCCGAAATCGGTGAAGGTGACAGTACTTTCCGATCGCACCACCAATATCCGCGCATCCGTCGATGATACTCAGTTTGAATTGATGATGGCTATCGCGCTGGTAGTCATGATTATCTACCTGTTTTTGCGCAATATTCCg              >  1:484023/1‑140 (MQ=255)                                                                                                                   ccGAAATCGGTGAAGGTGACAGTACTTTCCGATCGCACCACCAATATCCGCGCATCCGTCGATGATACTCAGTTTGAATTGATGATGGCTATCGCGCTGGTAGTCATGATTATCTACCTGTTTTTgcg                        >  1:804341/1‑128 (MQ=255)                                                                                                                   ccGAAATCGGTGAAGGTGACAGTACTTTCCGATCGCACCACCAATATCCGCGCATCCGTCGATGATACTCAGTTTGAATTGATGATGGCTATCGCGCTGGTAGTCATGATTATCTACCTGTTTTTgcg                        <  2:804341/128‑1 (MQ=255)                                                                                                                     gAAATCGGTGAAGGTGACAGTACTTTCCGATCGCACCACCAATATCCGCGCATCCGTCGATGATACTCAGTTTGAATTGATGATGGCTATCGCGCTGGTAGTCATGATTATCTACCTGTTTTTGCGCAATATTCCGGCGa          <  1:587075/140‑1 (MQ=255)                                                                                                                        aTCGGTGAAGGTGACAGTACTTTCCGATCGCACCACCAATATCCGCGCATCCGTCGATGATACTCAGTTTGAATTGATGATGGCTATCGCGCTGGTAGTCATGATTATCTACCTGTTTTTGCGCAATATTCCGGCGACca       <  2:484023/140‑1 (MQ=255)                                                                                                                         tCGGTGAAGGTGACAGTACTTTCCGATCGCACCACCAATATCCGCGCATCCGTCGATGATACTCAGTTTGAATTGATGATGGCTATCGCGCTGGTAGTCATGATTATCTACCTGTTTTTGCGCAATATTCCGGCGACcat      >  1:121319/1‑140 (MQ=255)                                                                                                                             tGAAGGTGACAGTACTTTCCGATCGCACCACCAATATCCGCGCATCCGTCGATGATACTCAGTTTGAATTGATGATGGCTATCGCGCTGGTAGTCATGATTATCTACCTGTTTTTGCGCAATATTCCGGCGACCATCAtt  <  1:178734/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                               TGGGCGAACAAAGAACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCACCACCAATATCCGCGCATCCGTCGATGATACTCAGTTTGAATTGATGATGGCTATCGCGCTGGTAGTCATGATTATCTACCTGTTTTTGCGCAATATTCCGGCGACCATCATT  >  NC_000913/2156109‑2156372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑