Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,177,020	A→G	L62P (CTG→CCG)	gatY ←	tagatose‑1,6‑bisphosphate aldolase 2 subunit GatY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,177,020	0	A	G	100.0%	94.3 / NA	27	L62P (CTG→CCG)	gatY	tagatose‑1,6‑bisphosphate aldolase 2 subunit GatY
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/13);  total (14/13)

CGAGGCGTCAATCATGACTGAGCGCACGCCAGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACAGATTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGC  >  NC_000913/2176887‑2177141                                                                                                                                      |                                                                                                                          cGAGGCGTCAATCATGACTGAGCGCACGCCAGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACGGAtttt                                                                                                                     <  1:456631/140‑1 (MQ=255) cGAGGCGTCAATCATGACTGAGCGCACGCCAGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACGGAtttt                                                                                                                     <  1:676438/140‑1 (MQ=255)       gTCAATCATGACTGAGCGCACGCCAGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACGGATTTTCTGTAc                                                                                                               <  2:545634/140‑1 (MQ=255)        tCAATCATGACTGAGCGCACGCCAGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACGGATTTTCTGTAcc                                                                                                              <  2:736827/140‑1 (MQ=255)                     aGCGCACGCCAGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACGGATTTTCTGTACCAGCATGAGTAAAt                                                                                                 >  2:728974/1‑140 (MQ=255)                                      acCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACGGATTTTCTGTACCAGCATGAGTAAATGTGcc                                                                                            <  2:664084/128‑1 (MQ=255)                                      acCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACGGATTTTCTGTACCAGCATGAGTAAATGTGcc                                                                                            >  1:664084/1‑128 (MQ=255)                                          tCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACGGATTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCgatg                                                                            >  2:207806/1‑140 (MQ=255)                                           cTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACGGATTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCgatga                                                                           >  2:359598/1‑140 (MQ=255)                                                        tCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACGGATTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATg                                                              <  1:359598/140‑1 (MQ=255)                                                                cGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACGGATTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTgg                                                      >  1:701527/1‑140 (MQ=255)                                                                 gtgtGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACGGATTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGgcg                                                                  <  1:300010/127‑1 (MQ=255)                                                                 gtgtGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACGGATTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGgcg                                                                  >  2:300010/1‑127 (MQ=255)                                                                   gtgATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACGGATTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAg                                                   <  2:557504/140‑1 (MQ=255)                                                                                tGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACGGATTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTaccac                                      <  1:207806/140‑1 (MQ=255)                                                                                 gAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACGGATTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTaccacc                                     >  1:399479/1‑140 (MQ=255)                                                                                   aTTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACGGATTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCgg                                                >  1:734918/1‑127 (MQ=255)                                                                                   aTTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACGGATTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCgg                                                <  2:734918/127‑1 (MQ=255)                                                                                        cAGTGGATGGCGATCTTGCTTCGCCATCGCGCTGACCAGCGCCAACGGATTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTGCa                              <  2:701527/140‑1 (MQ=255)                                                                                                       ttGCTTCGCCATCGCGCTGACCAGCGCCAACGGATTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATtgt               >  2:426617/1‑140 (MQ=255)                                                                                                          cTTCGCCATCGCGCTGACCAGCGCCAACGGATTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGaa            >  2:499103/1‑140 (MQ=255)                                                                                                          cTTCGCCATCGCGCTGACCAGCGCCAACGGATTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGaa            >  1:463130/1‑140 (MQ=255)                                                                                                           ttCGCCATCGCGCTGACCAGCGCCAACGGATTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAat           >  1:50440/1‑140 (MQ=255)                                                                                                            tCGCCATCGCGCTGACCAGCGCCAACGGATTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCgag                    >  1:383206/1‑130 (MQ=255)                                                                                                            tCGCCATCGCGCTGACCAGCGCCAACGGATTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCgag                    <  2:383206/130‑1 (MQ=255)                                                                                                            tCGCCATCGCGCTGACCAGCGCCAACGGATTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAata          >  1:807483/1‑140 (MQ=255)                                                                                                                    gcgcTGACCAGCGCCAACGGATTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGc  <  2:673347/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                          CGAGGCGTCAATCATGACTGAGCGCACGCCAGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACAGATTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGC  >  NC_000913/2176887‑2177141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑