Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,253,386	T→C	T254A (ACC→GCC)	nupX ←	putative nucleoside transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,253,386	0	T	C	100.0%	145.5 / NA	41	T254A (ACC→GCC)	nupX	putative nucleoside transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/22);  total (19/22)

ACCTAAAATGGACTCCAGCGAGGCATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGCAATTATTGCAACAAATGCCATCACCACTGTTGCCACACCTGCGGCGATTTTCAGCCCGGTCATTGCCCCTGTCGCAGCGGCTTCAATAATGCTTTTTGGCGGTGTTTCGGTGAAAGAGAGGTTATTAAAGGAAACCTGCGAAGATTCCGTTGCCGGGCTTAACAGGCGGGCAAACAAGATCCCGCCAGGGAT  >  NC_000913/2253249‑2253518                                                                                                                                          |                                                                                                                                     aCCTAAAATGGACTCCAGCGAGGCATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGCAATTATTGCAACAAATGCCATCACCACTGTTGCCACACCTGCGGCGATTTTCAGCCCGGCCa                                                                                                                                    <  1:808299/140‑1 (MQ=255)      aaaTGGACTCCAGCGAGGCATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGCAATTATTGCAACAAATGCCATCACCACTGTTGCCACACCTGCGGCGATTTTCAGCCCGGCCATTGcc                                                                                                                               <  2:511604/140‑1 (MQ=255)          ggACTCCAGCGAGGCATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGCAATTATTGCAACAAATGCCATCACCACTGTTGCCACACCTGCGGCGATTTTCAGCCCGGCCATTGCCCCTg                                                                                                                           <  2:14569/140‑1 (MQ=255)             cTCCAGCGAGGCATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGCAATTATTGCAACAAATGCCATCACCACTGTTGCCACACCTGCGGCGATTTTCAGCCCGGCCATTGCCCCTGTCg                                                                                                                        >  2:695470/1‑140 (MQ=255)             cTCCAGCGAGGCATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGCAATTATTGCAACAAATGCCATCACCACTGTTGCCACACCTGCGGCGATTTTCAGCCCGGCCATTGCCCCTGTCg                                                                                                                        >  2:512625/1‑140 (MQ=255)               ccAGCGAGGCATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGCAATTATTGCAACAAATGCCATCACCACTGTTGCCACACCTGCGGCGATTTTCAGCCCGGCCATTGCCCCTGTCGCa                                                                                                                      <  1:334382/140‑1 (MQ=255)                       gCATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGCAATTATTGCAACAAATGCCATCACCACTGTTGCCACACCTGCGGCGATTTTCAGCCCGGCCATTGCCCCTGTCGCAGCGGCTTc                                                                                                              >  1:511512/1‑140 (MQ=255)                         aTGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGCAATTATTGCAACAAATGCCATCACCACTGTTGCCACACCTGCGGCGATTTTCAGCCCGGCCATTGCCCCTGTCGCAGCGGCTTCaa                                                                                                            <  1:287659/140‑1 (MQ=255)                                            ccaccaACGCCGCCGATAATACCGTTAATCAACGCAATTATTGCAACAAATGCCATCACCACTGTTGCCACACCTGCGGCGATTTTCAGCCCGGCCATTGCCCCTGTCGCAGCGGCTTCAATAATGCTTTTTGGCGgtgt                                                                                         <  1:389168/140‑1 (MQ=255)                                              accaACGCCGCCGATAATACCGTTAATCAACGCAATTATTGCAACAAATGCCATCACCACTGTTGCCACACCTGCGGCGATTTTCAGCCCGGCCATTGCCCCTGTCGCAGCGGCTTCAATAATGCTTTTTGGCGGTGttt                                                                                       <  1:474022/140‑1 (MQ=255)                                                            taataCCGTTAATCAACGCAATTATTGCAACAAATGCCATCACCACTGTTGCCACACCTGCGGCGATTTTCAGCCCGGCCATTGCCCCTGTCGCAGCGGCTTCAATAATGCTTTTTGGCGGTGTTTCGGTGAAAGAGAgg                                                                         <  1:366380/140‑1 (MQ=255)                                                               taCCGTTAATCAACGCAATTATTGCAACAAATGCCATCACCACTGTTGCCACACCTGCGGCGATTTTCAGCCCGGCCATTGCCCCTGTCGCAGCGGCTTCAATAATGCTTTTTGGCGGTGTTTCGGTGAAAGAGAGGtta                                                                      <  1:735637/140‑1 (MQ=255)                                                                   gTTAATCAACGCAATTATTGCAACAAATGCCATCACCACTGTTGCCACACCTGCGGCGATTTTCAGCCCGGCCATTGCCCCTGTCGCAGCGGCTTCAATAATGCTTTTTGGCGGTGTTTCGGTGAAAGAGAGGTTATTaa                                                                  >  2:390961/1‑140 (MQ=255)                                                                      aaTCAACGCAATTATTGCAACAAATGCCATCACCACTGTTGCCACACCTGCGGCGATTTTCAGCCCGGCCATTGCCCCTGTCGCAGCGGCTTCAATAATGCTTTTTGGCGGTGTTTCGGTGaaa                                                                               <  2:559029/124‑1 (MQ=255)                                                                      aaTCAACGCAATTATTGCAACAAATGCCATCACCACTGTTGCCACACCTGCGGCGATTTTCAGCCCGGCCATTGCCCCTGTCGCAGCGGCTTCAATAATGCTTTTTGGCGGTGTTTCGGTGaaa                                                                               >  1:559029/1‑124 (MQ=255)                                                                      aaTCAACGCAATTATTGCAACAAATGCCATCACCACTGTTGCCACACCTGCGGCGATTTTCAGCCCGGCCATTGCCCCTGTCGCAGCGGCTTCAATAATGCTTTTTGGCGGTGTTTCGGTGAAAGAGAGGTTATTAAAgg                                                               >  1:319433/1‑140 (MQ=255)                                                                       aTCAACGCAATTATTGCAACAAATGCCATCACCACTGTTGCCACACCTGCGGCGATTTTCAGCCCGGCCATTGCCCCTGTCGCAGCGGCTTCAATAATGCTTTTTGGCGGTGTTTCGGTGAAAGAGAGGTTATTAAAGGa                                                              >  2:669692/1‑140 (MQ=255)                                                                            cGCAATTATTGCAACAAATGCCATCACCACTGTTGCCACACCTGCGGCGATTTTCAGCCCGGCCATTGCCCCTGTCGCAGCGGCTTCAATAATGCTTTTTGGCGGTGTTTCGGTGAAAGAGAGGTTATTAAAGGAAACCt                                                         >  1:141323/1‑140 (MQ=255)                                                                            cGCAATTATTGCAACAAATGCCATCACCACTGTTGCCACACCTGCGGCGATTTTCAGCCCGGCCATTGCCCCTGTCCCAGCGGCTTCAATAATGCTTTTTGGCGGTGTTTCGGTGAAAGAGAGGTTATTAAAGGAAACCt                                                         >  1:798487/1‑140 (MQ=255)                                                                                     tGCAACAAATGCCATCACCACTGTTGCCACACCTGCGGCGATTTTCAGCCCGGCCATTGCCCCTGTCGCAGCGGCTTCAATAATGCTTTTTGGCGGTGTTTCGGTGAAAGAGAGGTTATTAAAGGAAACCTGCGAAGAtt                                                >  1:215360/1‑140 (MQ=255)                                                                                      gCAACAAATGCCATCACCACTGTTGCCACACCTGCGGCGATTTTCAGCCCGGCCATTGCCCCTGTCGCAGCGGCTTCAATAATGCTTTTTGGCGGTGTTTCGGTGAAAGAGAGGTTATTAAAGGAAACCTGCGAAGATTc                                               >  2:264613/1‑140 (MQ=255)                                                                                             aTGCCATCACCACTGTTGCCACACCTGCGGCGATTTTCAGCCCGGCCACTGCCCCTGTCGCAGCGGCTTCAATAATGCTTTTTGGCGGTGTTTCGGTGAAAGAGAGGTTATTAAAGGAAACCTGCGAAGATTCCGTTGcc                                        >  1:103680/1‑140 (MQ=255)                                                                                              tGCCATCACCACTGTTGCCACACCTGCGGCGATTTTCAGCCCGGCCATTGCCCCTGTCGCAGCGGCTTCAATAATGCTTTTTGGCGGTGTTTCGGTGAAAGAGAGGTTATTAAAGGAAACCTGCGAAGATTCCGTTGCCg                                       >  2:685849/1‑140 (MQ=255)                                                                                              tGCCATCACCACTGTTGCCACACCTGCGGCGATTTTCAGCCCGGCCATTGCCCCTGTCGCAGCGGCTTCAATAATGCTTTTTGGCGGTGTTTCGGTGAAAGAGAGGTTATTAAAGGAAACCTGCGAAGATTCCGTTGCCg                                       <  2:511512/140‑1 (MQ=255)                                                                                               gCCATCACCACTGTTGCCACACCTGCGGCGATTTTCAGCCCGGCCATTGCCCCTGTCGCAGCGGCTTCAATAATGCTTTTTGGCGGTGTTTCGGTGAAAGAGAGGTTATTAAAGGAAACCTGCGAAGATTCCGTTGCCgg                                      >  2:57324/1‑140 (MQ=255)                                                                                                          tGTTGCCACACCTGCGGCGATTTTCAGCCCGGCCATTGCCCCTGTCGCAGCGGCTTCAATAATGCTTTTTGGCGGTGTTTCGGTGAAAGAGAGGTTATTAAAGGAAACCTGCGAAGATTCCGTTGCCGGGCTTAACAGGc                           <  2:127312/140‑1 (MQ=255)                                                                                                          tGTTGCCACACCTGCGGCGATTTTCAGCCCGGCCATTGCCCCTGTCGCAGCGGCTTCAATAATGCTTTTTGGCGGTGTTTCGGTGAAAGAGAGGTTATTAAAGGAAACCTGCGAAGATTCCGTTGCCGGGCTTAACAGGc                           <  1:512625/140‑1 (MQ=255)                                                                                                            ttGCCACACCTGCGGCGATTTTCAGCCCGGCCATTGCCCCTGTCGCAGCGGCTTCAATAATGCTTTTTGGCGGTGTTTCGGTGAAAGAGAGGTTATTAAAGGAAACCTGCGAAGATTCCGTTGCCGGGCTTAACAGGCgg                         <  2:141323/140‑1 (MQ=255)                                                                                                             tGCCACTCCTGCGGCGATTTTCAGCCCGGCCATTGCCCCTGTCGCAGCGGCTTCAATAATGCTTTTTGCCGGTGTTTCGGTGAAAGAGAGGTTATTAAAGGAAACCTGCGAAGATTCCGTTGCCGGGCTTAACAGGCggg                        <  2:562740/140‑1 (MQ=255)                                                                                                             tGCCACACCTGCGGCGATTTTCAGCCCGGCCATTGCCCCTGTCGCAGCGGCTTCAATAATGCTTTTTGGCGGTGTTTCGGTGAAAGAGAGGTTATTAAAGGAAACCTGCGAAGATTCCGTTGCCGGGCTTAACAGGCggg                        >  2:431374/1‑140 (MQ=255)                                                                                                              gCCACACCTGCGGCGATTTTCAGCCCGGCCATTGCCCCTGTCGCAGCGGCTTCAATAATGCTTTTTGGCGGTGTTTCGGTGAAAGAGAGGTTATTAAAGGAAACCTGCGAAGATTCCGTTGCCGGGCTTAACAGGCggg                        >  1:605415/1‑139 (MQ=255)                                                                                                              gCCACACCTGCGGCGATTTTCAGCCCGGCCATTGCCCCTGTCGCAGCGGCTTCAATAATGCTTTTTGGCGGTGTTTCGGTGAAAGAGAGGTTATTAAAGGAAACCTGCGAAGATTCCGTTGCCGGGCTTAACAGGCggg                        <  2:605415/139‑1 (MQ=255)                                                                                                               ccACACCTGCGGCGATTTTCAGCCCGGCCATTGCCCCTGTCGCAGCGGCTTCAATAATGCTTTTTGGCGGTGTTTCGGTGAAAGAGAGGTTATTAAAGGAAACCTGCGAAGATTCCGTTGCCGGGCTTaa                                <  2:29437/130‑1 (MQ=255)                                                                                                               ccACACCTGCGGCGATTTTCAGCCCGGCCATTGCCCCTGTCGCAGCGGCTTCAATAATGCTTTTTGGCGGTGTTTCGGTGAAAGAGAGGTTATTAAAGGAAACCTGCGAAGATTCCGTTGCCGGGCTTaa                                >  1:29437/1‑130 (MQ=255)                                                                                                                    ccTGCGGCGATTTTCAGCCCGGCCATTGCCCCTGTCGCAGCGGCTTCAATAATGCTTTTTGGCGGTGTTTCGGTGAAAGAGAGGTTATTAAAGGAAACCTGCGAAGATTCCGTTGCCGGGCTTAACAGGCGGGc                       <  2:348064/134‑1 (MQ=255)                                                                                                                    ccTGCGGCGATTTTCAGCCCGGCCATTGCCCCTGTCGCAGCGGCTTCAATAATGCTTTTTGGCGGTGTTTCGGTGAAAGAGAGGTTATTAAAGGAAACCTGCGAAGATTCCGTTGCCGGGCTTAACAGGCGGGc                       >  1:348064/1‑134 (MQ=255)                                                                                                                      tGCGGCGATTTTCAGCCCGGCCATTGCCCCTGTCGCAGCGGCTTCAATAATGCTTTTTGGCGGTGTTTCGGTGAAAGAGAGGTTATTAAAGGAAACCTGCGAAGATTCCGTTGCCGGGCTTAACAGGCGGGCAAACAAGa               <  1:288131/140‑1 (MQ=255)                                                                                                                      tGCGGCGATTTTCAGCCCGGCCATTGCCCCTGTCGCAGCGGCTTCAATAATGCTTTTTGGCGGTGTTTCGGTGAAAGAGAGGTTATTAAAGGAAACCTGCGAAGATTCCGTTGCCGGGCTTAACAGGCGGGCAAACAAGa               >  1:2464/1‑140 (MQ=255)                                                                                                                                   aGCCCGGCCATTGCCCCTGTCGCAGCGGCTTCAATAATGCTTTTTGGCGGTGTTTCGGTGAAAGAGAGGTTATTAAAGGAAACCTGCGAAGATTCCGTTGCCGGGCTTAACAGGCGGGCAAACAAGATCCCGCCAGGGAt  <  2:376590/140‑1 (MQ=255)                                                                                                                                   aGCCCGGCCATTGCCCCTGTCGCAGCGGCTTCAATAATGCTTTTTGGCGGTGTTTCGGTGAAAGAGAGGTTATTAAAGGAAACCTGCGAAGATTCCGTTGCCGGGCTTAACAGGCGGGCAAACAAGATCCCGCCAGGGAt  <  1:685849/140‑1 (MQ=255)                                                                                                                                   aGCCCGGCCATTGCCCCTGTCGCAGCGGCTTCAATAATGCTTTTTGGCGGTGTTTCGGTGAAAGAGAGGTTATTAAAGGAAACCTGCGAAGATTCCGTTGCCGGGCTTAACAGGCGGGCAAACAAGATCCCGCCAGGGAt  <  2:677070/140‑1 (MQ=255)                                                                                                                                   aGCCCGGCCATTGCCCCTGTCGCAGCGGCTTCAATAATGCTTTTTGGCGGTGTTTCGGTGAAAGAGAGGTTATTAAAGGAAACCTGCGAAGATTCCGTTGCCGGGCTTAACAGGCGGGCAAACAAGATCCCGCCAGGGAt  <  2:718014/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                     ACCTAAAATGGACTCCAGCGAGGCATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGCAATTATTGCAACAAATGCCATCACCACTGTTGCCACACCTGCGGCGATTTTCAGCCCGGTCATTGCCCCTGTCGCAGCGGCTTCAATAATGCTTTTTGGCGGTGTTTCGGTGAAAGAGAGGTTATTAAAGGAAACCTGCGAAGATTCCGTTGCCGGGCTTAACAGGCGGGCAAACAAGATCCCGCCAGGGAT  >  NC_000913/2253249‑2253518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑