Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,308,123	A→T	F165I (TTT→ATT)	yojI ←	ABC transporter family protein/microcin J25 efflux protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,308,123	0	A	T	100.0%	174.6 / NA	51	F165I (TTT→ATT)	yojI	ABC transporter family protein/microcin J25 efflux protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (24/27);  total (24/27)

AAACACATACTCGGCGCGTTCCCGGTTCAGAGTCAGCTCTTTGCGCCCTTCAAGTACAGTTTGAAAATCCGTGTACAGCTTGTCTTCGGTTTCACGCAGGGTCGCCATATGTTTGTACACCCGCGCCACCAGCACAAAACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTTCCGGCAGACGCACAAAAGC  >  NC_000913/2307986‑2308255                                                                                                                                          |                                                                                                                                     aaaCACATACTCGGCGCGTTCCCGGTTCAGAGTCAGCTCTTTGCGCCCTTCAAGTACAGTTTGAAAATCCGTGTACAGCTTGTCTTCGGTTTCACGCAGGGTCGCCATATGTTTGTACACCCGCGCCACCAGCACAATAc                                                                                                                                    <  1:409718/140‑1 (MQ=255)  aaCACATACTCGGCGCGTTCCCGGTTCAGAGTCAGCTCTTTGCGCCCTTCAAGTACAGTTTGAAAATCCGTGTACAGCTTGTCTTCGGTTTCACGCAGGGTCGCCATATGTTTGTACACCCGCGCCACCAGCACAATAcc                                                                                                                                   <  2:781795/140‑1 (MQ=255)  aaCACATACTCGGCGCGTTCCCGGTTCAGAGTCAGCTCTTTGCGCCCTTCAAGTACAGTTTGAAAATCCGTGTACAGCTTGTCTTCGGTTTCACGCAGGGTCGCCATATGTTTGTACACCCGCGCCACCAGCACAATAcc                                                                                                                                   <  1:768758/140‑1 (MQ=255)   acacATACTCGGCGCGTTCCCGGTTCAGAGTCAGCTCTTTGCGCCCTTCAAGTACAGTTTGAAAATCCGTGTACAGCTTGTCTTCGGTTTCACGCAGGGTCGCCATATGTTTGTACACCCGCGCCACCAGCACAATACCg                                                                                                                                  <  2:144498/140‑1 (MQ=255)      caTACTCGGCGCGTTCCCGGTTCAGAGTCAGCTCTTTGCGCCCTTCAAGTACAGTTTGAAAATCCGTGTACAGCTTGTCTTCGGTTTCACGCAGGGTCGCCATATGTTTGTACACCCGCGCCACCAGCACAATACCGccc                                                                                                                               <  2:776626/140‑1 (MQ=255)       aTACTCGGCGCGTTCCCGGTTCAGAGTCAGCTCTTTGCGCCCTTCAAGTACAGTTTGAAAATCCGTGTACAGCTTGTCTTCGGTTTCACGCAGGGTCGCCATATGTTTATACACCCGCGCCACCAGCACAATACCGcccc                                                                                                                              >  1:464958/1‑140 (MQ=255)            cGGCGCGTTCCCGGTTCAGAGTCAGCTCTTTGCGCCCTTCAAGTACAGTTTGAAAATCCGTGTACAGCTTGTCTTCGGTTTCACGCAGGGTCGCCATATGTTTGTACACCCGCGCCACCAGCACAATACCGCCCCAGATg                                                                                                                         >  1:63654/1‑140 (MQ=255)              gcgcGTTCCCGGTTCAGAGTCAGCTCTTTGCGCCCTTCAAGTACAGTTTGAAAATCCGTGTACAGCTTGTCTTCGGTTTCACGCAGGGTCGCCATATGTTTGTACACCCGCGCCACCAGCACAATACCGCCCCAGATGGt                                                                                                                       <  1:747141/140‑1 (MQ=255)               cgcgTTCCCGGTTCAGAGTCAGCTCTTTGCGCCCTTCAAGTACAGTTTGAAAATCCGTGTACAGCTTGTCTTCGGTTTCACGCAGGGTCGCCATATGTTTGTACACCCGCGCCACCAGCACAATACCGCCCCAGATGGt                                                                                                                       <  2:795003/139‑1 (MQ=255)               cgcgTTCCCGGTTCAGAGTCAGCTCTTTGCGCCCTTCAAGTACAGTTTGAAAATCCGTGTACAGCTTGTCTTCGGTTTCACGCAGGGTCGCCATATGTTTGTACACCCGCGCCACCAGCACAATACCGCCCCAGATGGt                                                                                                                       >  1:795003/1‑139 (MQ=255)               cgcgTTCCCGGTTCAGAGTCAGCTCTTTGCGCCCTTCAAGTACAGTTTGAAAATCCGTGTACAGCTTGTCTTCGGTTTCACGCAGGGTCGCCATATGTTTGTACACCCGCGCCACCAGCACAATACCGCCCCAGATGGTg                                                                                                                      >  1:86705/1‑140 (MQ=255)               cgcgTTCCCGGTTCAGAGTCAGCTCTTTGCGCCCTTCAAGTACAGTTTGAAAATCCGTGTACAGCTTGTCTTCGGTTTCACGCAGGGTCGCCATATGTTTGTACACCCGCGCCACCAGCACAATACCGCCCCAGATGGTg                                                                                                                      >  1:757031/1‑140 (MQ=255)                 cgTTCCCGGTTCAGAGTCAGCTCTTTGCGCCCTTCAAGTACAGTTTGAAAATCCGTGTACAGCTTGTCTTCGGTTTCACGCAGGGTCGCCATATGTTTGTACACCCGCGCCACCAGCACAATACCGCCCCAGATGGTGAt                                                                                                                    <  2:86705/140‑1 (MQ=255)                 cgTTCCCGGTTCAGAGTCAGCTCTTTGCGCCCTTCAAGTACAGTTTGAAAATCCGTGTACAGCTTGTCTTCGGTTTCACGCAGGGTCGCCATATGTTTGTACACCCGCGCCACCAGCACAATACCGCCCCAGATGGTGAt                                                                                                                    <  1:606194/140‑1 (MQ=255)                 cgTTCCCGGTTCAGAGTCAGCTCTTTGCGCCCTTCAAGTACAGTTTGAAAATCCGTGTACAGCTTGTCTTCGGTTTCACGCAGGGTCGCCATATGTTTATACACCCGCGCCACCAGCACAATACCGCCCCAGATGGTGAt                                                                                                                    <  2:464958/140‑1 (MQ=255)                   ttCCCGGTTCAGAGTCAGCTCTTTGCGCCCTTCAAGTACAGTTTGAAAATCCGTGTACAGCTTGTCTTCGGTTTCACGCAGGGTCGCCATATGTTTGTACACCCGCGCCACCAGCACAATACCGCCCCAGATGGTGATCg                                                                                                                  >  2:109097/1‑140 (MQ=255)                   ttCCCGGTTCAGAGTCAGCTCTTTGCGCCCTTCAAGTACAGGTTGAAAATCCGTGTACAGCTTGTCTTCGGTTTCACGCAGGGTCGCCATATGTTTGTACACCCGCGCCACCAGCACAATACCGCCCCAGATGGTGATCg                                                                                                                  >  2:40092/1‑140 (MQ=255)                     cccGGTTCAGAGTCAGCTCTTTGCGCCCTTCAAGTACAGTTTGAAAATCCGTGTACAGCTTGTCTTCGGTTTCACGCAGGGTCGCCATATGTTTGTACACCCGCGCCACCAGCACAATACCGCCCCAGATGGTGATCGcc                                                                                                                <  2:537954/140‑1 (MQ=255)                     cccGGTTCAGAGTCAGCTCTTTGCGCCCTTCAAGTACAGTTTGAAAATCCGTGTACAGCTTGTCTTCGGTTTCACGCAGGGTCGCCATATGTTTGTACACCCGCGCCACCAGCACAATACCGCCCCAGATGGTGATCGcc                                                                                                                <  2:63654/140‑1 (MQ=255)                       cGGTTCAGAGTCAGCTCTTTGCGCCCTTCAAGTACAGTTTGAAAATCCGTGTACAGCTTGTCTTCGGTTTCACGCAGGGTCGCCATATGTTTGTACACCCGCGCCACCAGCACAATACCGCCCCAGATGGTGATCGCCAt                                                                                                              <  1:109097/140‑1 (MQ=255)                                          tGCGCCCTTCAAGTACAGTTTGAAAATCCGTGTACAGCTTGTCTTCGGTTTCACGCAGGGTCGCCATATGTTTGTACACCCGCGCCACCAGCACAATACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGc                                                                                           >  2:650412/1‑140 (MQ=255)                                              cccTTCAAGTACAGTTTGAAAATCCGTGTACAGCTTGTCTTCGGTTTCACGCAGGGTCGCCATATGTTTGTACACCCGCGCCACCAGCACAATACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACa                                                                                       <  1:659034/140‑1 (MQ=255)                                                          aGTTTGAAAATCCGTGTACAGCTTGTCTTCGGTTTCACGCAGGGTCGCCATATGTTTGTACACCCGCGCCACCAGCACAATACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACa                                                                           <  2:51764/140‑1 (MQ=255)                                                          aGTTTGAAAATCCGTGTACAGCTTGTCTTCGGTTTCACGCAGGGTCGCCATATGTTTGTACACCCGCGCCACCAGCACAATACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACa                                                                           <  2:716776/140‑1 (MQ=255)                                                           gTTTGAAAATCCGTGTACAGCTTGTCTTCGGTTTCACGCAGGGTCGCCATATGTTTGTACACCCGCGCCACCAGCACAATACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTg                                                                                  <  1:83946/132‑1 (MQ=255)                                                           gTTTGAAAATCCGTGTACAGCTTGTCTTCGGTTTCACGCAGGGTCGCCATATGTTTGTACACCCGCGCCACCAGCACAATACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTg                                                                                  >  2:83946/1‑132 (MQ=255)                                                             ttGAAAATCCGTGTACAGCTTGTCTTCGGTTTCACGCAGGGTCGCCATATGTTTGTACACCCGCGCCACCAGCACAATACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACAGCa                                                                        <  1:377743/140‑1 (MQ=255)                                                                               cTTGTCTTCGGTTTCACGCAGGGTCGCCATATGTTTGTACACCCGCGCCACCAGCACAATACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTg                                                      >  1:674017/1‑140 (MQ=255)                                                                                  gTCTTCGGTTTCACGCAGGGTCGCCATATGTTTGTACACCCGCGCCACCAGCACAATACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAAc                                                   >  1:562814/1‑140 (MQ=255)                                                                                      tCGGTTTCACGCAGGGTCGCCATATGTTTGTACACCCGCGCCACCAGCACAATACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGAt                                               <  1:472337/140‑1 (MQ=255)                                                                                       cGGTTTCACGCAGGGTCGCCATATGTTTGTACACCCGCGCCACCAGCACAATACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATa                                              >  2:479328/1‑140 (MQ=255)                                                                                           ttCACGCAGGGTCGCCATATGTTTGTACACCCGCGCCACCAGCACAATACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTga                                          <  1:11994/140‑1 (MQ=255)                                                                                              aCGCAGGGTCGCCATATGTTTGTACACCCGCGCCACCAGCACAATACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGaa                                       <  2:782240/140‑1 (MQ=255)                                                                                                                   gTACACCCGCGCCACCAGCACAATACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTTCCg                  >  1:561032/1‑140 (MQ=255)                                                                                                                       acCCGCGCCACCAGCACAATACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTTCCGGCAg              >  1:448787/1‑140 (MQ=255)                                                                                                                        cccGCGCCACCAGCACAATACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTTCCGGCAGa             >  2:174846/1‑140 (MQ=255)                                                                                                                        cccGCGCCACCAGCACAATACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTTCCGGCAGa             >  1:629497/1‑140 (MQ=255)                                                                                                                        cccGCGCCACCAGCACAATACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTTCCGGCAGa             >  2:509280/1‑140 (MQ=255)                                                                                                                        cccGCGCCACCAGCACAATACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTTCCGGCAGa             >  1:245502/1‑140 (MQ=255)                                                                                                                        cccGCGCCACCAGCACAATACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTTCCGGCAGa             >  1:451654/1‑140 (MQ=255)                                                                                                                          cgcgCCACCAGCACAATACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTTCCGGCAGACg           <  2:561032/140‑1 (MQ=255)                                                                                                                            cgcCACCAGCACAATACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTTCCGGCAGACGca         >  1:202931/1‑140 (MQ=255)                                                                                                                            cgcCACCAGCACAATACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTTCCGGCAGACGca         >  1:750892/1‑140 (MQ=255)                                                                                                                            cgcCACCAGCACAATACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTTCCGGCAGACGca         >  1:21648/1‑140 (MQ=255)                                                                                                                            cgcCACCAGCACAATACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTTCCGGCAGACGca         >  2:606978/1‑140 (MQ=255)                                                                                                                            cgcCACCAGCACAATACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTTCCGGCAGACGca         >  1:614622/1‑140 (MQ=255)                                                                                                                             gcCACCAGCACAATACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTTCCGGCAGACGcac        <  1:644631/140‑1 (MQ=255)                                                                                                                                  caGCACAATACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTTCCGGCAGACGCACAAAAg   <  1:481015/140‑1 (MQ=255)                                                                                                                                   aGCACAATACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTTCCGGCAGACGCACAAAAGc  <  1:485129/140‑1 (MQ=255)                                                                                                                                   aGCACAATACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTTCCGGCAGACGCACAAAAGc  <  1:421008/140‑1 (MQ=255)                                                                                                                                   aGCACAATACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTTCCGGCAGACGCACAAAAGc  <  1:509280/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                     AAACACATACTCGGCGCGTTCCCGGTTCAGAGTCAGCTCTTTGCGCCCTTCAAGTACAGTTTGAAAATCCGTGTACAGCTTGTCTTCGGTTTCACGCAGGGTCGCCATATGTTTGTACACCCGCGCCACCAGCACAAAACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTTCCGGCAGACGCACAAAAGC  >  NC_000913/2307986‑2308255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑