Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,431,944	T→C	L333L (CTA→CTG)	folC ←	bifunctional folylpolyglutamate synthetase/dihydrofolate synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,431,944	0	T	C	100.0%	54.1 / NA	17	L333L (CTA→CTG)	folC	bifunctional folylpolyglutamate synthetase/dihydrofolate synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/4);  total (13/4)

TCAAATGATTTGCCGTTACCCAAATGCTCAAGCAGTTGTTCTGCCGTGGCACCGCGCGGCCCTTCCAGTGGCGCACAATACCAGTCATCAACCACGCTTTTCAACCAGGCCAGAGTTCCGGCAATATCTTTATCATGTAGCATACCGATAACCGCCAGCACGCGCCCGTTTTTCGGTAGCGCTTTCATACGCCCGGTGAGATATTCCGCCGCATGTGGATTATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGAAA  >  NC_000913/2431807‑2432076                                                                                                                                          |                                                                                                                                     tCAAATGATTTGCCGTTACCCAAATGCTCAAGCAGTTGTTCTGCCGTGGCACCGCGCGGCCCTTCCAGTGGCGCACAATACCAGTCATCAACCACGCTTTTCAACCAGGCCAGAGTTCCGGCAATATCTTTATCATGCAg                                                                                                                                    >  1:285673/1‑140 (MQ=255)  cAAATGATTTGCCGTTACCCAAATGCTCAAGCAGTTGTTCTGCCGTGGCACCGCGCGGCCCTTCCAGTGGCGCACAATACCAGTCATCAACCACGCTTTTCAACCAGGCCAGAGTTCCGGCAATATCTTTATCATGCAGc                                                                                                                                   <  1:531836/140‑1 (MQ=255)  cAAATGATTTGCCGTTACCCAAATGCTCAAGCAGTTGTTCTGCCGTGGCACCGCGCGGCCCTTCCAGTGGCGCACAATACCAGTCATCAACCACGCTTTTCAACCAGGCCAGAGTTCCGGCAATATCTTTATCATGCAGc                                                                                                                                   <  1:579922/140‑1 (MQ=255)   aaaTGATTTGCCGTTACCCAAATGCTCAAGCAGTTGTTCTGCCGTGGCACCGCGCGGCCCTTCCAGTGGCGCACAATACCAGTCATCAACCACGCTTTTCAACCAGGCCAGAGTTCCGGCAATATCTTTATCATgcagca                                                                                                                                  <  1:9631/140‑1 (MQ=255)      tGATTTGCCGTTACCCAAATGCTCAAGCAGTTGTTCTGCCGTGGCACCGCGCGGCCCTTCCAGTGGCGCACAATACCAGTCATCAACCACGCTTTTCAACCAGGCCAGAGTTCCGGCAATATCTTTATCATGCAGCATAc                                                                                                                               >  1:658665/1‑140 (MQ=255)                        aTGCTCAAGCAGTTGTTCTGCCGTGGCACCGCGCGGCCCTTCCAGTGGCGCACAATACCAGTCATCAACCACGCTTTTCAACCAGGCCAGAGTTCCGGCAATATCTTTATCATGCAGCATACCGATAACCGCCAGCAcgc                                                                                                             >  1:632847/1‑140 (MQ=255)                        aTGCTCAAGCAGTTGTTCTGCCGTGGCACCGCGCGGCCCTTCCAGTGGCGCACAATACCAGTCATCAACCACGCTTTTCAACCAGGCCAGAGTTCCGGCAATATCTTTATCATGCAGCATACCGATAACCGCCAGCAcgc                                                                                                             >  1:88287/1‑140 (MQ=255)                                   gttgttCTGCCGTGGCACCGCGCGGCCCTTCCAGTGGCGCACAATACCAGTCATCAACCACGCTTTTCAACCAGGCCAGAGTTCCGGCAATATCTTTATCATGCAGCATACCGATAACCGCCAGCACGCGCCCGTTTTTc                                                                                                  >  1:511587/1‑140 (MQ=255)                                    ttgttCTGCCGTGGCACCGCGCGGCCCTTCCAGTGGCGCACAATACCAGTCATCAACCACGCTTTTCAACCAGGCCAGAGTTCCGGCAATATCTTTATCATGCAGCATACCGATAACCGCCAGCACGCGCCCGTTTTTCg                                                                                                 >  2:12899/1‑140 (MQ=255)                                       ttCTGCCGGGCCCCCGCGCGGCCCTTCCAGGGGCCCAAAATCCCAGTCACCAACCCCCTTTTTCACCCAGGCCAGAGTCCCGGCAATTTTTTTATCATGCAGCATCCCAATAACCGCCAGCACGCGCCCGTTTTTCGGTa                                                                                              <  2:617699/140‑1 (MQ=255)                                           gCCGTGGCCCCGCGCGCCCTTTCCAGTGGCGCAAAATCCCAGTCATCAACCCCGCTTTTCACCCGGGCCAGAGTTCCGACAATATTTTTATCATGCAGCATACCAAAAACCGCCAGCACGCGCCCGTTTTTCGGTAgcgc                                                                                          <  2:70770/140‑1 (MQ=255)                                                      gcgcgGCCCTTCCAGTGGCGCAAAATACCAGTCATCACCCGCGCTTTTCACCCAGGCCAGAGTTCCGGCAATATCTTTATCATGCAGCATCCCGATAACCGCCAGCACGCGCCCGTTTTTCGGTAGCGCTTTCATACGcc                                                                               <  2:88287/140‑1 (MQ=255)                                                       cgcgGCCCTTCCAGTGGCGCACAATACCAGTCATCAACCACGCTTTTCAACCAGGCCAGAGTTCCGGCAATATCTTTATCATGCAGCATACCGATAACCGCCAGCACGCGCCCGTTTTTCGGTAGCGCTTTCATACGccc                                                                              >  1:95139/1‑140 (MQ=255)                                                           gCCCTACCAGTGGCGCAAAATACCAGTCATAACCCTCGATTTGCAACCGGGCCAGAGTCCCGGCAATTTCTTTATCATACAGTTTACGGAAACCCGCCAGCACGCGCCCGTTTTTCGGTAGCGCTTTCATACGCCCGGTg                                                                          <  2:641958/140‑1 (MQ=255)                                                                     gggCGCAAAATACCAGTCATCACCCACGTTTTTCAACCAGGCCAGAGTCCCGGCAATATCTTTATCATGCAGCATACCGATAACCGCCAGCACGCGCCCGTTTTTCGGTAGCGCTTTCATACGCCCGGTGAGATATTccg                                                                <  2:511587/139‑1 (MQ=255)                                                                      ggCGCACAATACCAGTCATCACCCACGCTTTCCACCCAGGCCAGAGTCCCGGCAATATTTTTATCATGCACCATCCCGAAAACCGCCAGCACGCGCCCGTTTTTCGGTAGCGCTTTCATACGCCCGGTGAGATATTccgc                                                               <  2:647980/140‑1 (MQ=255)                                                                          cacaATACCAGTCATCAACCACGCTTTTCAACCAGGCCAGAGTTCCGGCAATATCTTTATCATGCAGCATACCGATAACCGCCAGCACGCGCCCGTTTTTCGGTAGCGCTTTCATACGCCCGGTGAGATATTCCGCCGCa                                                           >  2:375009/1‑140 (MQ=255)                                                                          cacaATACCAGTCATCAACCACGCTTTTCAACCAGGCCAGAGTTCCGGCAATATCTTTATCATGCAGCATACCGATAACCGCCAGCACGCGCCCGTTTTTCGGTAGCGCTTTCATACGCCCGGTGAGATATTCCGCCGCa                                                           >  2:722952/1‑140 (MQ=255)                                                                                  cAGTCATCAACCACGCTTTTCAACCAGGCCAGAGTTCCGGCAATATCTTTATCATGCAGCATACCGATAACCGCCAGCACGCGCCCGTTTTTCGGTAGCGCTTTCATACGCCCGGTGAGATATTCCGCCGCATGTGGAtt                                                   >  1:132005/1‑140 (MQ=255)                                                                                                gCTTTTCAACCAGGCCAGAGTTCCGGCAATATCTTTATCATGCAGCATACCGATAACCGCCAGCACGCGCCCGTTTTTCGGTAGCGCTTTCATACGCCCGGTGAGATATTCCGCCGCATGTGGATTATGCGCGACATCaa                                     >  1:534943/1‑140 (MQ=255)                                                                                                                 gagTCCCGGCAATATTTTTATCATGCAGCATACGAATAACCGCCAGCACGCGCCCGTTTTTCGGTAGCGCTTTCATACGCCCGGTGAGATATTCCGCCGCATGTGGATTATGCGCGACATCAAAAATAACGCGTGGCGAc                    <  2:54916/140‑1 (MQ=255)                                                                                                                       cGGCAATATCTTTATCATGCAGCATACCGATAACCGCCAGCACGCGCCCGTTTTTCGGTAGCGCTTTCATACGCCCGGTGAGATATTCCGCCGCATGTGGATTATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTc              >  1:463498/1‑140 (MQ=255)                                                                                                                        ggCAATTTTTTTATCATGCAGCATCCCGATAACCGCCAGCACGCGCCCGTTTTTCGGTAGCGCTTTCATACGCCCGGTGAGATATTCCGCCGCATGTGGATTATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTca             <  2:632847/140‑1 (MQ=255)                                                                                                                        ggCAATATTTTTATCAGGCACCATACCAATAACCGCCAGCACGCGCCCGTTTTTCGGTAGCGCTTTCATACGCCCGGTGAGATATTCCGCCGCATGTGGATTATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTca             <  2:195089/140‑1 (MQ=255)                                                                                                                        ggCAATATCTTTCTCAGCCACCATACGAATAACCGCCAGCACGCGCCCGTTTTTCGGTAGCGCTTTCATACGCCCGGTGAGATATTCCGCCGCATGTGGATTATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTca             <  2:463498/140‑1 (MQ=255)                                                                                                                            atatCTTTATCATGCAGCATACCGATAACCGCCAGCACGCGCCCGTTTTTCGGTAGCGCTTTCATACGCCCGGTGAGATATTCCGCCGCATGTGGATTATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTCACAAt         <  2:285673/140‑1 (MQ=255)                                                                                                                                   tATCATGCAGCATACCGATAACCGCCAGCACGCGCCCGTTTTTCGGTAGCGCTTTCATACGCCCGGTGAGATATTCCGCCGCATGTGGATTATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGaaa  >  2:565684/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                     TCAAATGATTTGCCGTTACCCAAATGCTCAAGCAGTTGTTCTGCCGTGGCACCGCGCGGCCCTTCCAGTGGCGCACAATACCAGTCATCAACCACGCTTTTCAACCAGGCCAGAGTTCCGGCAATATCTTTATCATGTAGCATACCGATAACCGCCAGCACGCGCCCGTTTTTCGGTAGCGCTTTCATACGCCCGGTGAGATATTCCGCCGCATGTGGATTATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGAAA  >  NC_000913/2431807‑2432076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑