Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	445,303	(T)6→5	coding (1364/1365 nt)	yajR ←	putative major facilitator superfamily transport protein YajR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	445,298	0	T	.	100.0%	67.7 / NA	17	intergenic (+124/+4)	yajQ/yajR	nucleotide binding protein/putative major facilitator superfamily transport protein YajR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (10/7);  total (10/7)

ATCGCGACTATGTGCGTTTTGTTCATGTCAGATGCGGCATGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACG  >  NC_000913/445174‑445427                                                                                                                             |                                                                                                                                  aTCGCGACTATGTGCGTTTTGTTCATGTCAGATGCGGCATGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCG‑TTTTTATGCCTGACGa                                                                                                                   <  2:231184/140‑1 (MQ=255)           tgtgCGTTTTGTTCATGTCAGATGCGGCATGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCG‑TTTTTATGCCTGACGAATTGCCTGtt                                                                                                         >  1:408142/1‑140 (MQ=255)             tgCGTTTTGTTCATGTCAGATGCGGCATGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCG‑TTTTTATGCCTGACGAATTGCCTGTTCt                                                                                                       >  2:126300/1‑140 (MQ=255)                         aTGTCAGATGCGGCATGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCG‑TTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGa                                                                                           >  2:107518/1‑140 (MQ=255)                                     gCATGAACACCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGGAGGCCTGATAAGCGAAGCGCAGCAGGCAATTCGCCGCG‑TTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCTTCACtttt                                                                               >  2:241313/1‑139 (MQ=255)                                      cATGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCG‑TTTTTATGCCTGACGAATTGCTTGTTCTATCTCAAAGCGATTCGTCACTTTGc                                                                              >  1:330456/1‑140 (MQ=255)                                              ccTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCG‑TTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGAtt                                                                      <  2:258329/140‑1 (MQ=255)                                                                      tAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCG‑TTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGttcttct                                              >  2:171374/1‑140 (MQ=255)                                                                         ttCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCG‑TTTTTATGCCTGACGAATTGCTTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTtcttcttct                                           <  2:330456/140‑1 (MQ=255)                                                                                 cTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCG‑TTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCaa                                   <  1:171374/140‑1 (MQ=255)                                                                                 cTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCG‑TTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCaa                                   <  1:394062/140‑1 (MQ=255)                                                                                    tGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCG‑TTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAacac                                >  2:90673/1‑140 (MQ=255)                                                                                              aTAAGCGTAGCGCAGCAGGCAATTCGCCGCG‑TTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAAt                                      >  2:143163/1‑124 (MQ=255)                                                                                              aTAAGCGTAGCGCAGCAGGCAATTCGCCGCG‑TTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAAt                                      <  1:143163/124‑1 (MQ=255)                                                                                              aTAAGCGTAGCGCAGCAGGCAATTCGCCGCG‑TTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATg                      >  1:205209/1‑140 (MQ=255)                                                                                              aTAAGCGTAGCGCAGCAGGCAATTCGCCGCG‑TTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATg                      >  2:357360/1‑140 (MQ=255)                                                                                                             cagGCAATTCGCCGCG‑TTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGc       >  2:328929/1‑140 (MQ=255)                                                                                                                  aaTTCGCCGCG‑TTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACg  <  2:31881/140‑1 (MQ=255)                                                                                                                             |                                                                                                                                  ATCGCGACTATGTGCGTTTTGTTCATGTCAGATGCGGCATGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACG  >  NC_000913/445174‑445427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑