Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	995,967	(A)5→4	coding (22/1146 nt)	ssuD ←	FMNH2‑dependent alkanesulfonate monooxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	995,963	0	A	.	100.0%	81.4 / NA	20	coding (26/1146 nt)	ssuD	FMNH2‑dependent alkanesulfonate monooxygenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (13/7);  total (13/7)

AGCGCCCCGTTGGAATTAGCACACCGGTATAGCCAAGACGATCCGCCGCTTGCGCAATTTGTTGCAGATAACCGTGATCAACCGGGCGTGAACCTTCTTCCGTTCCCAGATAATGCCCGTCACCGTGGGTCGGTAAAAACCAGAACATATTCAGACTCATAATTGTTTTCCTTCCAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTTGCT  >  NC_000913/995829‑996088                                                                                                                                       |                                                                                                                              aGCGCCCCGTTGGAATTAGCACACCGGTATAGCCAAGACGATCCGCCGCTTGCGCAATTTGTTGCAGATAACCGTGATCAACCGGGCGTGAACCTTCTTCCGTTCCCAGATAATGCCCGTCACCGTGGGTCGGTaaaacc                                                                                                                          <  2:352913/140‑3 (MQ=255)                             ataGCCAAGACGATCCGCCGCTTGCGCAATTTGTTGCAGATAACCGTGATCAACCGGGCGTGAACCTTCTTCCGTTCCCAGATAATGCCCGTCACCGTGGGTCGGT‑AAAACCAGAACATATTCAGACTCATAATTGtttt                                                                                             <  2:334793/140‑1 (MQ=255)                             ataGCCAAGACGATCCGCCGCTTGCGCAATTTGTTGCAGATAACCGTGATCAACCGGGCGTGAACCTTCTTCCGTTCCCAGATAATGCCCGTCACCGTGGGTCGGT‑AAAACCAGAACATATTCAGACTCATAATTGtttt                                                                                             <  2:249728/140‑1 (MQ=255)                                 ccAAGACGATCCGCCGCTTGCGCAATTTGTTGCAGATAACCGTGATCAACCGGGCGTGAACCTTCTTCCGTTCCCAGATAATGCCCGTCACCGTGGGTCGGT‑AAAACCAGAACATATTCAGACTCATAATTGTTttcctt                                                                                         >  1:329242/1‑140 (MQ=255)                                     gACGATCCGCCGCTTGCGCAATTTGTTGCAGATAACCGTGATCAACCGGGCGTGAACCTTCTTCCGTTCCCAGATAATGCCCGTCACCGTGGGTCGGT‑AAAACCAGAACATATTCAGACTCATAATTGTTTTCCTTCCAg                                                                                     >  1:123469/1‑140 (MQ=255)                                     gACGATCCGCCGCTTGCGCAATTTGTTGCAGATAACCGTGATCAACCGGGCGTGAACCTTCTTCCGTTCCCAGATAATGCCCGTCACCGTGGGTCGGT‑AAAACCAGAACATATTCAGACTCATAATTGTTTTCCTTCCAg                                                                                     >  1:163045/1‑140 (MQ=255)                                     gACGATCCGCCGCTTGCGCAATTTGTTGCAGATAACCGTGATCAACCGGGCGTGAACCTTCTTCCGTTCCCAGATAATGCCCGTCACCGTGGGTCGGT‑AAAACCAGAACATATTCAGACTCATAATTGTTTTCCTTCCAg                                                                                     >  2:10213/1‑140 (MQ=255)                                      aCGATCCGCCGCTTGCGCAATTTGTTGCAGATAACCGTGATCAACCGGGCGTGAACCTTCTTCCGTTCCCAGATAATGCCCGTCACCGTGGGTCGGT‑AAAACCAGAACATATTCAGACTCATAATTGTTTTCCTTCCAGt                                                                                    >  1:182009/1‑140 (MQ=255)                                          tCCGCCGCTTGCGCAATTTGTTGCAGATAACCGTGATCAACCGGGCGTGAACCTTCTTCCGTTCCCAGATAATGCCCGTCACCGTGGGTCGGT‑AAAACCAGAACATATTCAGACTCATAATTGTTTTCCTTCCAGTTGAg                                                                                >  1:406619/1‑140 (MQ=255)                                           ccgccgCTTGCGCAATTTGTTGCAGATAACCGTGATCAACCGGGCGTGAACCTTCTTCCGTTCCCAGATAATGCCCGTCACCGTGGGTCGGT‑AAAACCAGAACATATTCAGACTCATAATTGTTTTCCTTCCAGTTGAGt                                                                               <  1:10213/140‑1 (MQ=255)                                               cgcTTGCGCAATTTGTTGCAGATAACCGTGATCAACCGGGCGTGAACCTTCTTCCGTTCCCAGATAATGCCCGTCACCGTGGGTCGGT‑AAAACCAGAACATATTCAGACTCATAATTGTTttccttc                                                                                        <  1:342562/127‑1 (MQ=255)                                               cgcTTGCGCAATTTGTTGCAGATAACCGTGATCAACCGGGCGTGAACCTTCTTCCGTTCCCAGATAATGCCCGTCACCGTGGGTCGGT‑AAAACCAGAACATATTCAGACTCATAATTGTTttccttc                                                                                        >  2:342562/1‑127 (MQ=255)                                                     cgcAATTTGTTGCAGATAACCGTGATCAACCGGGCGTGAACCTTCTTCCGTTCCCAGATAATGCCCGTCACCGTGGGTCGGT‑AAAACCAGAACATATTCAGACTCATAATTGTTTTCCTTCCAGTTGAGTGGGCTGCCAg                                                                     >  1:31536/1‑140 (MQ=255)                                                                                                tcttcCGTTCCCAGATAATGCCCGTCACCGTGGGTCGGT‑AAAACCAGAACATATTCAGACTCATAATTGTTTTCCTTCCAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGAtttt                          >  2:66285/1‑140 (MQ=255)                                                                                                                    cccGTCACCGTGGGTCGGT‑AAAACCAGAACATATTCAGACTCATAATTGTTTTCCTTCCAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGtt      <  1:323204/140‑1 (MQ=255)                                                                                                                    cccGTCACCGTGGGTCGGT‑AAAACCAGAACATATTCAGACTCATAATTGTTTTCCTTCCAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGtt      <  2:163045/140‑1 (MQ=255)                                                                                                                    cccGTCACCGTGGGTCGGT‑AAAACCAGAACATATTCAGACTCATAATTGTTTTCCTTCCAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGtt      <  2:329242/140‑1 (MQ=255)                                                                                                                    cccGTCACCGTGGGTCGGT‑AAAACCAGAACATATTCAGACTCATAATTGTTTTCCTTCCAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGtt      >  1:163175/1‑140 (MQ=255)                                                                                                                     ccGTCACCGTGGGTCGGT‑AAAACCAGAACATATTCAGACTCATAATTGTTTTCCTTCCAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGttt     >  1:14802/1‑140 (MQ=255)                                                                                                                        tCACCGTGGGTCGGT‑AAAACCAGAACATATTCAGACTCATAATTGTTTTCCTTCCAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTtgct  >  2:122148/1‑140 (MQ=255)                                                                                                                        tCACCGTGGGTCGGT‑AAAACCAGAACATATTCAGACTCATAATTGTTTTCCTTCCAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTtgct  >  2:63356/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                              AGCGCCCCGTTGGAATTAGCACACCGGTATAGCCAAGACGATCCGCCGCTTGCGCAATTTGTTGCAGATAACCGTGATCAACCGGGCGTGAACCTTCTTCCGTTCCCAGATAATGCCCGTCACCGTGGGTCGGTAAAAACCAGAACATATTCAGACTCATAATTGTTTTCCTTCCAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTTGCT  >  NC_000913/995829‑996088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑