Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,818,550	(C)7→6	intergenic (‑50/+55)	chbF ← / ← chbR	monoacetylchitobiose‑6‑phosphate hydrolase/DNA‑binding transcriptional dual regulator ChbR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,818,544	0	C	.	100.0%	98.6 / NA	24	intergenic (‑44/+61)	chbF/chbR	monoacetylchitobiose‑6‑phosphate hydrolase/DNA‑binding transcriptional dual regulator ChbR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (10/14);  total (10/14)

GCAATTCGTGATAACGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGCCCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGATAGCTCTTAGGCGTAAAGGACGTTAATTTCTTAA  >  NC_000913/1818407‑1818669                                                                                                                                          |                                                                                                                              gCAATTCGTGATAACGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGccc                                                                                                                             >  1:194657/1‑140 (MQ=255)       cGTGATAACGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGccccccgga                                                                                                                       >  1:418975/1‑137 (MQ=255)           aTAACGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑CCCCCCGGACata                                                                                                                  >  2:75609/1‑140 (MQ=255)                  ttAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑CCCCCCGGACATAAATAATc                                                                                                           >  2:375706/1‑140 (MQ=255)                     ataaATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑CCCCCCGGACATAAATAATCCAg                                                                                                        <  1:213906/140‑1 (MQ=255)                          tCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGccccccggac                                                                                                                      >  1:237275/1‑118 (MQ=255)                          tCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGccccccggac                                                                                                                      <  2:237275/122‑5 (MQ=255)                          tCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑CCCCCCGGACATAAATAATCCAGCAACa                                                                                                   >  1:125292/1‑140 (MQ=255)                                 aGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑CCCCCCGGACATAAATAATCCAGAAACAGGACAGa                                                                                            <  1:365291/140‑1 (MQ=255)                                     aCTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑CCCCCCGGACATAAATAATCCAGCAACAGGACAGATAtg                                                                                        >  1:229391/1‑140 (MQ=255)                                       tCCGGGGTATAGTTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑CCCCCCGGACATAAATAATCCAGCAACAGGACAGATAtgtg                                                                                      <  2:7462/140‑1 (MQ=255)                                            ggTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑CCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTg                                                                                 >  1:105257/1‑140 (MQ=255)                                             gTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑CCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGt                                                                                <  2:229391/140‑1 (MQ=255)                                              tataGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑CCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTc                                                                               <  1:375706/140‑1 (MQ=255)                                                   ctgctCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑CCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGta                                                                          <  2:194657/140‑1 (MQ=255)                                                      ctCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑CCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATaa                                                                       >  2:18099/1‑140 (MQ=255)                                                            ccaccaATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑CCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACtt                                                                 <  2:183657/140‑1 (MQ=255)                                                              accaATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑CCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTAc                                                               <  2:349642/140‑1 (MQ=255)                                                                         cgacTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑CCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTc                                                    >  2:351749/1‑140 (MQ=255)                                                                             ttttAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑CCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTc                                                >  2:261039/1‑140 (MQ=255)                                                                                    ttCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑CCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCt                                         <  2:235004/140‑1 (MQ=255)                                                                                                aTTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑CCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGATAGctct                             <  1:351888/140‑1 (MQ=255)                                                                                                 tttCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑CCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGATAGCTCtt                            <  2:125292/140‑1 (MQ=255)                                                                                                 tttCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑CCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGATAGCTCtt                            <  2:418975/140‑1 (MQ=255)                                                                                                    ctcCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑CCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGATAGCTCTTAgg                         <  1:261039/140‑1 (MQ=255)                                                                                                                      aCTGATATCTGGCATATCTG‑CCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGATAGCTCTTAGGCGTAAAGGACGTTAAttt       >  2:203662/1‑140 (MQ=255)                                                                                                                        tGATATCTGGCATATCTG‑CCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGATAGCTCTTAGGCGTAAAGGACGTTAATTTCt     >  2:14418/1‑140 (MQ=255)                                                                                                                           tatCTGGCATATCTG‑CCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGATAGCTCTTAGGCGTAAAGGACGTTAATTTCTTaa  <  2:105257/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                              GCAATTCGTGATAACGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGCCCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGATAGCTCTTAGGCGTAAAGGACGTTAATTTCTTAA  >  NC_000913/1818407‑1818669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑