Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,264,341	Δ1 bp	coding (479/1182 nt)	setB →	sugar exporter SetB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,264,339	0	C	.	100.0%	28.3 / NA	8	coding (477/1182 nt)	setB	sugar exporter SetB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (2/6);  total (2/6)

CTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTACGGTAATGTATCTGAGCGCAGCGGTAGCGTTTATTGTTTGCG  >  NC_000913/2264213‑2264421                                                                                                                               |                                                                                   cTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGGTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGG‑CCACCGCTGGCTTa                                                                      <  1:396075/140‑1 (MQ=255)           aCCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGG‑CCACCGCTGGCTTATGCCTTAGCg                                                            >  1:318320/1‑140 (MQ=255)            ccGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGG‑CCACCGCTGGCTTATGCCTTAGCGa                                                           <  2:312930/140‑1 (MQ=255)                        aaaTGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGG‑CCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCt                                               <  1:190734/140‑1 (MQ=255)                                       cccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGG‑CCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTACGGTAATGTATc                                >  1:261076/1‑140 (MQ=255)                                                                 cGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGG‑CCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTACGGTAATGTATCTGAGCGCAGCGGTAGCGTTTATTGtt      <  2:277298/140‑1 (MQ=255)                                                                   tGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGG‑CCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTACGGTAATGTATCTGAGCGCAGCGGTAGCGTTTATTGTTTg    <  1:214564/140‑1 (MQ=255)                                                                     aGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGG‑CCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTACGGTAATGTATCTGAGCGCAGCGGTAGCGTTTATTGTTTGCg  <  2:306746/140‑1 (MQ=255)                                                                                                                               |                                                                                   CTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTACGGTAATGTATCTGAGCGCAGCGGTAGCGTTTATTGTTTGCG  >  NC_000913/2264213‑2264421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑