| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,781,358 | +G | coding (144/474 nt) | trmL → | tRNA (cytidine/uridine‑2'‑O)‑ribose methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,781,354 | 1 | . | G | 95.7% | 79.2 / ‑2.9 | 23 | coding (140/474 nt) | trmL | tRNA (cytidine/uridine‑2'‑O)‑ribose methyltransferase |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (14/8); total (14/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
ATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGC‑GC‑GGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCGTAAGCTATC > NC_000913/3781215‑3781488 | aTGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCcgc‑gc < 2:337559/140‑1 (MQ=255) tttACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGC‑GCGGGGGCTGGACTATCa > 1:68144/1‑140 (MQ=255) atcCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGC‑GCGGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATcgcg > 2:122397/1‑140 (MQ=255) atcCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGC‑GCGGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATcgcg < 2:380470/140‑1 (MQ=255) aaTACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGC‑GCGGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAg > 2:194901/1‑140 (MQ=255) tCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGC‑GCGGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAg > 1:58437/1‑140 (MQ=255) catcGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGC‑GCGGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGcc < 1:228541/140‑1 (MQ=255) aaCCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGC‑GCGGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGcc < 2:295443/135‑1 (MQ=255) aaCCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGC‑GCGGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGcc > 1:295443/1‑135 (MQ=255) cccGATGGGTTTTGCCTGGGACGATAAGCGCCTGCGCCGCGGG‑GGGGCGGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTcacc < 2:335872/139‑1 (MQ=255) gATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGC‑GCGGGGGCTGGACTATCACGATTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACg < 1:194901/140‑1 (MQ=255) aTGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGC‑GCGGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGa > 1:65317/1‑140 (MQ=255) gCCTGGGACGATAAGCGCCTGCGCCGC‑GCGGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAgg > 2:363787/1‑135 (MQ=255) gCCTGGGACGATAAGCGCCTGCGCCGC‑GCGGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAgg < 1:363787/135‑1 (MQ=255) gCCTGGGACGATAAGCGCCTGCGCCGC‑GCGGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTacac < 2:58437/140‑1 (MQ=255) ggACGATAAGCGCCTGCGCCGC‑GCGGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAgg > 2:126369/1‑130 (MQ=255) ggACGATAAGCGCCTGCGCCGC‑GCGGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAgg < 1:308243/130‑1 (MQ=255) ggACGATAAGCGCCTGCGCCGC‑GCGGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAgg < 1:126369/130‑1 (MQ=255) ggACGATAAGCGCCTGCGCCGC‑GCGGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAgg > 2:308243/1‑130 (MQ=255) ggACGATAAGCGCCTGCGCCGC‑GCGGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCt > 2:228215/1‑140 (MQ=255) ggACGATAAGCGCCTGCGCCGC‑GCGGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCt > 1:258678/1‑140 (MQ=255) gcCTGCGCCGC‑GCGGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCGt < 2:258678/140‑1 (MQ=255) cccTGCGCCGC‑GCGGGGGCTGGACTATCACGATTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCGt < 2:68144/139‑1 (MQ=255) ccTGCGCCGC‑GCGGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCGTa > 2:26192/1‑140 (MQ=255) gcgcCGC‑GCGGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCGTAAGc < 1:122397/140‑1 (MQ=255) cgccgc‑GCGGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCGTAAGCt > 1:378852/1‑140 (MQ=255) cgccgc‑GCGGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCGTAAGCt > 1:101553/1‑140 (MQ=255) cgc‑gcgGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCGTAAGCTATc < 2:101553/140‑1 (MQ=255) | ATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGC‑GC‑GGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCGTAAGCTATC > NC_000913/3781215‑3781488 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |