Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,271,406	(G)5→6	coding (444/1101 nt)	chaA ←	Na(+)/K(+):H(+) antiporter ChaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,271,401	1	.	G	93.3%	46.8 / ‑2.7	15	coding (449/1101 nt)	chaA	Na(+)/K(+):H(+) antiporter ChaA
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base G (5/9);  total (5/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01

ATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCA‑GGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGGTGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGAATAGAGCG  >  NC_000913/1271271‑1271533                                                                                                                                    |                                                                                                                                     aTGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTATGATTATCGCCA‑ggggggaac                                                                                                                             >  1:94130/1‑136 (MQ=255)   gCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTATGATTATCGCCAGGGGGGAACAg                                                                                                                            <  1:336692/140‑1 (MQ=255)     gTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTATGATTATCGCCAGGGGGGAACAgcg                                                                                                                          <  1:394136/140‑1 (MQ=255)     gTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTATGATTATCGCCAGGGGGGAACAgcg                                                                                                                          <  2:125144/140‑1 (MQ=255)               ggATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTATGATTATCGCCAGGGGGGAACAGCGCAATTAAATa                                                                                                                <  1:68921/140‑1 (MQ=255)                                                                tAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTATGATTATCGCCAGGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGGTGGCAAACTTACGACCg                                                               >  1:162125/1‑140 (MQ=255)                                                                      cTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTATGATTATCGCCA‑GGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGGTGGCAAACTTACGACCGCCcaaca                                                        <  2:67640/140‑1 (MQ=255)                                                                       tGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTATGATTATCGCCAGGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGGTGGCAAACTTACGACCGCCcaaca                                                        >  2:328595/1‑140 (MQ=255)                                                                         aCCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTATGATTATCGCCAGGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGGTGGCAAACTTACGACCGCCCAACaat                                                      >  2:314096/1‑140 (MQ=255)                                                                              ttGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTATGATTATCGCCAGGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGGTGGCAAACTTACGACCGCCCAACAATAATGa                                                 >  1:277722/1‑140 (MQ=255)                                                                                       tCGCCGCAGGCAGAGCCATTGGAAATACCAGTATGATTATCGCCAGGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGGTGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAAc                                        <  2:277722/140‑1 (MQ=255)                                                                                            gcaggcagAGCCATTGGAAATACCAGTATGATTATCGCCAGGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGGAGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGcc                                   <  1:139046/140‑1 (MQ=255)                                                                                                                        tgATTATCGCCAGGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGGTGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGAATa       <  1:314096/139‑1 (MQ=255)                                                                                                                             aTCGCCAGGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGGTGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGa          <  1:103542/132‑1 (MQ=255)                                                                                                                             aTCGCCAGGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGGTGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGa          >  2:103542/1‑132 (MQ=255)                                                                                                                             aTCGCCAGGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGGTGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGAATAGAGCg  <  2:162125/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                     ATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCA‑GGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGGTGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGAATAGAGCG  >  NC_000913/1271271‑1271533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑