Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,263,924	(T)5→6	coding (62/1182 nt)	setB →	sugar exporter SetB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,263,919	1	.	T	100.0%	23.6 / NA	9	A19A (GCG→GCT)	setB	sugar exporter SetB
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (6/3);  total (6/3)

TCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCG‑TTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGATTCTTCTTTACCGGCAGCGCTG  >  NC_000913/2263837‑2264046                                                                                    |                                                                                                                                tcttcctcttccAGAGATGGACCAGCATGCATTACTCCCCCGCAGTCAACAGCGCGAAATCGATTGACCTGACCTCGACAGCGTTTTTTAATCGTTGACTTTGTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACt                                                                         <  2:192033/140‑1 (MQ=255)           ccAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTAttttt                                                               >  2:185873/1‑140 (MQ=255)              gTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACt                                                                         <  1:208085/127‑1 (MQ=255)              gTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACt                                                                         >  2:208085/1‑127 (MQ=255)                     aCCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATg                                                     <  2:225115/140‑1 (MQ=255)                                    tCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTc                                      >  1:137694/1‑140 (MQ=255)                                             gTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTTAATCGTTGCCTTTCTCACCGGCATTGCGGGCGCTCTGAAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGAtgg                             >  1:304884/1‑140 (MQ=255)                                                     cgcgAAATCGTTTGACCTGACCTCGACGGCGTTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGAttc                     >  1:396650/1‑140 (MQ=255)                                                                    cctgacctCGACGGCGTTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGATTCTTCTTTACCGGCAgc      <  2:137694/140‑1 (MQ=255)                                                                        acctCGACGGCGTTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGATTCTTCTTTACCGGCAGCGCTg  >  2:53276/1‑140 (MQ=255)                                                                                    |                                                                                                                                TCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCG‑TTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGATTCTTCTTTACCGGCAGCGCTG  >  NC_000913/2263837‑2264046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑