Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,297,114	(C)7→8	coding (531/624 nt)	ccmA ←	heme trafficking system ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,297,107	1	.	C	100.0%	67.2 / NA	19	I180V (ATT→GTT)	ccmA	heme trafficking system ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (8/11);  total (8/11)

CGCTATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAAT‑CCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGTCAGCCACAGACGCGCTAAAGCGACGCG  >  NC_000913/2296970‑2297239                                                                                                                                           |                                                                                                                                     cgcTATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAAT‑cc                                                                                                                                    <  1:251209/140‑1 (MQ=255)      tGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAAT‑ccccccc                                                                                                                               >  1:41818/1‑140 (MQ=255)      tGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCCGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAAT‑ccccccc                                                                                                                               <  2:176897/140‑1 (MQ=255)                cTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAAT‑cccccccct                                                                                                                             >  2:34767/1‑130 (MQ=255)                cTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAAT‑cccccccct                                                                                                                             <  1:34767/132‑3 (MQ=255)                     cGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTAtgctg                                                                                                                 <  1:62016/140‑1 (MQ=255)                        aGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTgcgc                                                                                                              >  2:35888/1‑140 (MQ=255)                        aGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTgcgc                                                                                                              >  2:358204/1‑140 (MQ=255)                                  ggAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTgg                                                                                                    >  1:181309/1‑140 (MQ=255)                                       aTGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAg                                                                                               <  1:358204/140‑1 (MQ=255)                                         gcgcCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTc                                                                                                 <  1:59344/136‑1 (MQ=255)                                         gcgcCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTc                                                                                                 >  2:59344/1‑136 (MQ=255)                                             cAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATc                                                                                         <  2:181309/140‑1 (MQ=255)                                                aaCATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGac                                                                                      <  2:41818/140‑1 (MQ=255)                                                    tcatGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCg                                                                                  <  1:35888/140‑1 (MQ=255)                                                       tGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTa                                                                               >  2:41050/1‑140 (MQ=255)                                                               tCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAAt                                                                       >  1:358888/1‑140 (MQ=255)                                                               tCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAAt                                                                       >  1:281189/1‑140 (MQ=255)                                                                 gTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATcg                                                                     <  2:281189/140‑1 (MQ=255)                                                                              aaaTGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCtc                                                        <  1:143827/140‑1 (MQ=255)                                                                                               cTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACg                                       <  1:396260/140‑1 (MQ=255)                                                                                                        aCGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACggg                              <  2:172678/140‑1 (MQ=255)                                                                                                                                aGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGTCAGCCACAGACGCGCTAAAGCGa      >  1:196560/1‑140 (MQ=255)                                                                                                                                    tCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGTCAGCCACAGACGCGCTAAAGCGAcgcg  <  2:196560/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                     CGCTATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAAT‑CCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGTCAGCCACAGACGCGCTAAAGCGACGCG  >  NC_000913/2296970‑2297239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑