| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,695,585 | (G)7→8 | intergenic (‑42/‑216) | purL ← / → mltF | phosphoribosylformylglycinamide synthetase/membrane‑bound lytic murein transglycosylase F |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,695,578 | 1 | . | G | 94.7% | 63.8 / ‑2.8 | 19 | intergenic (‑35/‑223) | purL/mltF | phosphoribosylformylglycinamide synthetase/membrane‑bound lytic murein transglycosylase F |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (8/10); total (9/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGTAAATATTGTGAACCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATC > NC_000913/2695441‑2695699 | cGTAAATATTGTGAACCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑‑gg < 1:35235/140‑1 (MQ=255) aaTATTGTGAACCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑‑gg < 1:347779/136‑1 (MQ=255) aaTATTGTGAACCGGGAGCCTGGCAGCCTGAAAACGCGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑‑gg > 2:347779/1‑136 (MQ=255) aaCCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTT‑CATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGc > 1:243769/1‑140 (MQ=255) ccGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCg < 2:184214/140‑1 (MQ=255) tGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGGAAACGGGCGTCATtata > 2:346660/1‑140 (MQ=255) cAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGa < 1:287372/140‑1 (MQ=255) tGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTg > 2:10445/1‑140 (MQ=255) gATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCg < 1:215321/140‑1 (MQ=255) tCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGa > 1:48803/1‑140 (MQ=255) cAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCa < 1:53878/140‑1 (MQ=255) tGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCAt > 2:91486/1‑140 (MQ=255) aaCCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAAtt > 2:405862/1‑140 (MQ=255) ccACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAg > 1:113029/1‑140 (MQ=255) catcatAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTgccgcc < 2:113029/140‑1 (MQ=255) tcatAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCaa < 1:405862/140‑1 (MQ=255) atAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATg < 2:243769/140‑1 (MQ=255) atAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATg < 1:288890/140‑1 (MQ=255) ctcGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCaa < 1:362098/130‑1 (MQ=255) ctcGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCaa > 2:362098/1‑130 (MQ=255) aGCTTTCGGTGTACCCAAGGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCAt < 1:346660/140‑1 (MQ=255) aGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATc > 1:385313/1‑140 (MQ=255) | CGTAAATATTGTGAACCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATC > NC_000913/2695441‑2695699 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |