Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	2,857,096	Δ2,538 bp	coding (4‑2541/2562 nt)	mutS →	DNA mismatch repair protein MutS

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	2857096	2859633	2538	22 [0]	[0] 22	mutS	DNA mismatch repair protein MutS

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 2857095	0 (0.000)	22 (0.890)	19/274	0.3	100%	coding (3/2562 nt)	mutS	DNA mismatch repair protein MutS
	?	NC_000913	2859634 =	0 (0.000)					coding (2542/2562 nt)	mutS	DNA mismatch repair protein MutS

GAACTCGCCGTTCGCTTCTTCCCCTGAAATGATTAACTCCGG‑TATCATGTGCGCCTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTATCTCAGGCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTT  >  NC_000913/2856970‑2857211                                                                                                                               |                                                                                                                     gAACTCGCCGTTCGCTTCTTCCCCTGAAATGATTAACTCCGGATATCATGTGCGCCTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcc                                                                                                         <  2:326738‑M1/140‑14 (MQ=255)      cGCCGTTCGCTTCTTCCCCTGAAATGATTAACTCCGG‑TATCATGTGCGCCTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgt                                                                                                   <  1:310210‑M1/140‑20 (MQ=255)       gCCGTTCGCTTCTTCCCCTGAAATGATTAACTCCGG‑TATCATGTGCGCCTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgta                                                                                                  >  2:209438‑M1/1‑120 (MQ=255)             cGCTTCTTCCCCTGAAATGATTAACTCCGG‑TATCATGTGCGCCTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataaca                                                                                            <  2:285720‑M1/140‑27 (MQ=255)                                      tCCGG‑TATCATGTGCGCCTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgg                                                                   <  1:369877‑M1/140‑52 (MQ=255)                                          g‑TATCATGTGCGCCTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtctt                                                                             <  2:372902‑M1/126‑42 (MQ=255)                                          g‑TATCATGTGCGCCTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtctt                                                                             >  1:372902‑M1/1‑85 (MQ=255)                                                        cTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaatattaacgacaact                                                  >  2:221635‑M1/1‑72 (MQ=255)                                                        cTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaatattaacgacaact                                                  >  2:205577‑M1/1‑72 (MQ=255)                                                                 ttACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaatattaacgacaactgacgaataa                                         >  2:168761‑M1/1‑63 (MQ=255)                                                                    cAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaatattaacgacaactgacgaataaaat                                      >  2:369465‑M1/1‑60 (MQ=255)                                                                     aaCGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaatattaacgacaactgacgaataa                                         >  2:98018‑M1/1‑59 (MQ=255)                                                                     aaCGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaatattaacgacaactgacgaataa                                         <  1:98018‑M1/136‑78 (MQ=255)                                                                       cGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaatattaacgacaactgacgaataaaataaa                                   >  2:159102‑M1/1‑57 (MQ=255)                                                                        gAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaatattaacgacaactgacgaataaaataaaa                                  <  1:175484‑M1/140‑85 (MQ=255)                                                                             tAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaatattaacgacaactgacgaataaaataaaaacacc                             <  2:160513‑M1/140‑90 (MQ=255)                                                                             tAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaatattaacgacaactgacgaataaaataaaaacacc                             <  1:369465‑M1/140‑90 (MQ=255)                                                                                aaaCCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaatattaacgacaactgacgaataaaataaaaacaccctg                          <  1:55935‑M1/140‑93 (MQ=255)                                                                                aaaCCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaatattaacgacaactgacgaataaaataaaaacaccctg                          <  1:221635‑M1/140‑93 (MQ=255)                                                                                          acCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaatattaacgacaactgacgaataaaataaaaacaccctgtataatagga                <  2:65052‑M1/140‑103 (MQ=255)                                                                                                    atatCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaatattaacgacaactgacgaataaaataaaaacaccctgtataataggaaagcttattt      >  2:138512‑M1/1‑28 (MQ=255)                                                                                                        cAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaatattaacgacaactgacgaataaaataaaaacaccctgtataataggaaagcttattttaca  >  2:306845‑M1/1‑24 (MQ=255)                                                                                                                               |                                                                                                                     GAACTCGCCGTTCGCTTCTTCCCCTGAAATGATTAACTCCGG‑TATCATGTGCGCCTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTATCTCAGGCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTT  >  NC_000913/2856970‑2857211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑