Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,989,120	T→C	L207L (TTG→CTG)	yqeI →	putative transcriptional regulator YqeI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,989,120	0	T	C	100.0%	82.4 / NA	24	L207L (TTG→CTG)	yqeI	putative transcriptional regulator YqeI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/13);  total (11/13)

GCGCATTTGTCATAGGCGCATATTCTGCATATTGGCTATGGAATAATAATCAACCGAAGCCATTTTTCAAGGATTACAAGACTGTTGCGGAAATTAATGGCTGCCATTTTAATGTAACAGAAGATACAATTGATGGGTTGAAAGAGTTCGATAAATATAAAACACGGATACTGGATTCGGGGATTAACTGTAAAAAACATCCGTGGTTATACTTCCCTCTTGCAAAATCCTCACCGGGGATGATTGTTATGGCATGCAATAAAAACTATAAC  >  NC_000913/2988983‑2989254                                                                                                                                          |                                                                                                                                       gcgcATTTGTCATAGGCGCATATTCTGCATATTGGCTATGGAATAATAATCAACCGAAGCCATTTTTCAAGGATTACAAGACTGTTGCGGAAATTATTGGCTGCCATTTTAATGTAACAGAAGATACAATTGATGGGCTg                                                                                                                                      >  2:234600/1‑140 (MQ=255) gcgcATTTGTCATAGGCGCATATTCTGCATATTGGCTATGGAATAATAATCAACCGAAGCCATTTTTCAAGGATTACAAGACTGTTGCGGAAATTAATGGCTGCCATTTTAATGTAACAGAAGATACAATTGATGGGCTg                                                                                                                                      >  1:205402/1‑140 (MQ=255) gcgcATTTGTCATAGGCGCATATTCTGCATATTGGCTATGGAATAATAATCAACCGAAGCCATTTTTCAAGGATTACAAGACTGTTGCGGAAATTAATGGCTGCCATTTTAATGTAACAGAAGATACAATTGATGGGCTg                                                                                                                                      >  1:80352/1‑140 (MQ=255)  cgcATTTGTCATAGGCGCATATTCTGCATATTGGCTATGGAATAATAATCAACCGAAGCCATTTTTCAAGGATTACAAGACTGTTGCGGAAATTAATGGCTGCCATTTTAATGTAACAGAAGATACAATTGATGGGCTGa                                                                                                                                     >  2:304660/1‑140 (MQ=255)             tAGGCGCATATTCTGCATATTGGCTATGGAATAATAATCAACCGAAGCCATTTTTCAAGGATTACAAGACTGTTGCGGAAATTAATGGCTGCCATTTTAATGTAACAGAAGATACAATTGATGGGCTGAAAGAGTTCGAt                                                                                                                          <  2:80352/140‑1 (MQ=255)                 cgcATATTCTGCATATTGGCTATGGAATAATAATCAACCGAAGCCATTTTTCAAGGATTACAAGACTGTTGCGGAAATTAATGGCTGCCATTTTAATGTAACAGAAGATACAATTGATGGGCTGAAAGAGTTCGATAAat                                                                                                                      >  1:153799/1‑140 (MQ=255)                     tatTCTGCATATTGGCTATGGAATAATAATCAACCGAAGCCATTTTTCAAGGATTACAAGACTGTTGCGGAAATTAATGGCTGCCATTTTAATGTAACAGAAGATACAATTGATGGGCTGAAAGAGTTCGATAAATATaa                                                                                                                  >  2:115192/1‑140 (MQ=255)                       ttCTGCATATTGGCTATGGAATAATAATCAACCGAAGCCATTTTTCAAGGATTACAAGACTGTTGCGGAAATTAATGGCTGCCATTTTAATGTAACAGAAGATACAATTGATGGGCTGAAAGAGTTCGATAAATATaaaa                                                                                                                <  2:381755/140‑1 (MQ=255)                          tGCATATTGGCTATGGAATAATAATCAACCGAAGCCATTTTTCAAGGATTACAAGACTGTTGCGGAAATTAATGGCTGCCATTTTAATGTAACAGAAGATACAATTGATGGGCTGAAAGAGTTCGATAAATATAAAacac                                                                                                             <  1:115192/140‑1 (MQ=255)                           gCATATTGGCTATGGAATAATAATCAACCGAAGCCATTTTTCAAGGATTACAAGACTGTTGCGGAAATTAATGGCTGCCATTTTAATGTAACAGAAGATACAATTGATGGGCTGAAAGAGTTCGATAAATATAAAACACg                                                                                                            <  2:153799/140‑1 (MQ=255)                                 tGGCTATGGAATAATAATCAACCGAAGCCATTTTTCAAGGATTACAAGACTGTTGCGGAAATTAATGGCTGCCATTTTAATGTAACAGAAGATACAATTGATGGGCTGAAAGAGTTCGATAAATATAAAACACGGATACt                                                                                                      >  2:360319/1‑140 (MQ=255)                                  ggCTATGGAATAATAATCAACCGAAGCCATTTTTCAAGGATTACAAGACTGTTGCGGAAATTAATGGCTGCCATTTTAATGTAACAGAAGATACAATTGATGGGCTGAAAGAGTTCGATAAATATAAAACACGGATACTg                                                                                                     >  1:252411/1‑140 (MQ=255)                                                aatCAACCGAAGCCATTTTTCAAGGATTACAAGACTGTTGCGGAAATTAATGGCTGCCATTTTAATGTAACAGAAGATACAATTGATGGGCTGAAAGAGTTCGATAAATATAAAACACGGATACTGGATTCGGGGATTaa                                                                                       <  1:360319/140‑1 (MQ=255)                                                      ccGAAGCCATTTTTCAAGGATTACAAGACTGTTGCGGAAATTAATGGCTGCCATTTTAATGTAACAGAAGATACAATTGATGGGCTGAAAGAGTTCGATAAATATAAAACACGGATACTGGATTCGGGGATTAACTGTaa                                                                                 >  2:241529/1‑140 (MQ=255)                                                         aaGCCATTTTTCAAGGATTACAAGACTGTTGCGGAAATTAATGGCTGCCATTTTAATGTAACAGAAGATACAATTGATGGGCTGAAAGAGTTCGATAAATATAAAACACGGATACTGGATTCGGGGATTAACTGTaaaaa                                                                              >  1:268019/1‑140 (MQ=255)                                                              aTTTTTCAAGGATTACAAGACTGTTGCGGAAATTAATGGCTGCCATTTTAATGTAACAGAAGATACAATTGATGGGCTGAAAGAGTTCGATAAATATAAAACACGGATACTGGATTCGGGGATTAACTGTAAAAAACATc                                                                         <  2:205402/140‑1 (MQ=255)                                                              aTTTTTCAAGGATTACAAGACTGTTGCGGAAATTAATGGCTGCCATTTTAATGTAACAGAAGATACAATTGATGGGCTGAAAGAGTTCGATAAATATAAAACACGGATACTGGATTCGGGGATTAACTGTAAAAAACATc                                                                         <  2:176367/140‑1 (MQ=255)                                                              aTTTTTCAAGGATTACAAGACTGTTGCGGAAATTAATGGCTGCCATTTTAATGTAACAGAAGATACAATTGATGGGCTGAAAGAGTTCGATAAATATAAAACACGGATACTGGATTCGGGGATTAACTGTAAAAAACATc                                                                         <  1:304665/140‑1 (MQ=255)                                                                                          gAAATTAATGGCTGCCATTTTAATGTAACAGAAGATACAATTGATGGGCTGAAAGAGTTCGATAAATATAAAACACGGATACTGGATTCGGGGATTAACTGTAAAAAACATCCGTGGTTATACTTCCCTCTTGCAAAATc                                             <  1:304660/140‑1 (MQ=255)                                                                                              ttAATGGCTGCCATTTTAATGTAACAGAAGATACAATTGATGGGCTGAAAGAGTTCGATAAATATAAAACACGGATACTGGATTCGGGGATTAACTGTAAAAAACATCCGTGGTTATACTTCCCTCTTGCAAAATCCTCa                                         <  2:25177/140‑1 (MQ=255)                                                                                                                    aaCAGAAGATACAATTGATGGGCTGAAAGAGTTCGATAAATATAAAACACGGATACTGGATTCGGGGATTAACTGTAAAAAACATCCGTGGTTATACTTCCCTCTTGCAAAATCCTCACCGGGGATGATTGTTATGGCAt                   <  1:241529/140‑1 (MQ=255)                                                                                                                           gaTACAATTGATGGGCTGAAAGAGTTCGATAAATATAAAACACGGATACTGGATTCGGGGATTAACTGTAAAAAACATCCGTGGTTATACTTCCCTCTTGCAAAATCCTCACCGGGGATGATTGTTATGGCATGCAATaa            <  2:268019/140‑1 (MQ=255)                                                                                                                                     aTGGGCTGAAAGAGTTCGATAAATATAAAACACGGATACTGGATTCGGGGATTAACTGTAAAAAACATCCGTGGTTATACTTCCCTCTTGCAAAATCCTCACCGGGGATGATTGTTATGGCATGCAATAAAAACtat     >  2:182133/1‑137 (MQ=255)                                                                                                                                     aTGGGCTGAAAGAGTTCGATAAATATAAAACACGGATACTGGATTCGGGGATTAACTGTAAAAAACATCCGTGGTTATACTTCCCTCTTGCAAAATCCTCACCGGGGATGATTGTTATGGCATGCAATAAAAACtat     <  1:182133/137‑1 (MQ=255)                                                                                                                                     aTGGGCTGAAAGAGTTCGATAAATATAAAACACGGATACTGGATTCGGGGATTAACTGTAAAAAACATCCGTGGTTATACTTCCCTCTTGCAAAATCCTCACCGGGGATGATTGTTATGGCATGCAATAAAAACTATAAc  >  2:36756/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       GCGCATTTGTCATAGGCGCATATTCTGCATATTGGCTATGGAATAATAATCAACCGAAGCCATTTTTCAAGGATTACAAGACTGTTGCGGAAATTAATGGCTGCCATTTTAATGTAACAGAAGATACAATTGATGGGTTGAAAGAGTTCGATAAATATAAAACACGGATACTGGATTCGGGGATTAACTGTAAAAAACATCCGTGGTTATACTTCCCTCTTGCAAAATCCTCACCGGGGATGATTGTTATGGCATGCAATAAAAACTATAAC  >  NC_000913/2988983‑2989254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑