Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,080,104	T→C	D511G (GAC→GGC)	tktA ←	transketolase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,080,104	0	T	C	100.0%	130.9 / NA	36	D511G (GAC→GGC)	tktA	transketolase 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/14);  total (22/14)

GAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCA  >  NC_000913/3079974‑3080239                                                                                                                                   |                                                                                                                                        gAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGCCCTGACGCt                                                                                                                                <  2:415147/140‑1 (MQ=255)     aTCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGCCCTGACGCTCAac                                                                                                                            <  2:209686/140‑1 (MQ=255)          ttCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGCCCTGACGCTCAACACCGt                                                                                                                       <  1:293974/140‑1 (MQ=255)                  gACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGCCCTGACGCTCAACACCGTATTTCCAc                                                                                                               >  2:365067/1‑140 (MQ=255)                    ccGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGCCCTGACGCTCAACACCGt                                                                                                                       >  2:285494/1‑130 (MQ=255)                    ccGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGCCCTGACGCTCAACACCGt                                                                                                                       <  1:285494/130‑1 (MQ=255)                                      acaTAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGCCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTg                                                                                           >  1:19740/1‑140 (MQ=255)                                         tAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGCCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTc                                                                                        >  2:6374/1‑140 (MQ=255)                                         tAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGCCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTc                                                                                        <  2:389391/140‑1 (MQ=255)                                          aaCCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGTGGTCGGGCCGCCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTca                                                                                       >  1:343951/1‑140 (MQ=255)                                          aaCCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGCCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTca                                                                                       >  1:371765/1‑140 (MQ=255)                                                  cgcgcgATGTTTGCCAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGCCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGt                                                                                         >  1:404837/1‑130 (MQ=255)                                                  cgcgcgATGTTTGCCAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGCCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGt                                                                                         <  2:404837/130‑1 (MQ=255)                                                  cgcgcgATGTTTGCCAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGCCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACg                                                                               >  1:354840/1‑140 (MQ=255)                                                  cgcgcgATGTTTGCCAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGCCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACg                                                                               <  1:365067/140‑1 (MQ=255)                                                  cgcgcgATGTTTGCCAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGCCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACg                                                                               >  1:123494/1‑140 (MQ=255)                                                  cgcgcgATGTTTGCCAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGCCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACg                                                                               <  2:123494/140‑1 (MQ=255)                                                  cgcgcgATGTTTGCCAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGCCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACg                                                                               >  2:174422/1‑140 (MQ=255)                                                    cgcgATGTTTGCCAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGCCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGcc                                                                             <  1:212264/140‑1 (MQ=255)                                                    cgcgATGTTTGCCAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGCCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGcc                                                                             <  2:354840/140‑1 (MQ=255)                                                    cgcgATGTTTGCCAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGCCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGcc                                                                             >  2:212264/1‑140 (MQ=255)                                                      cgATGTTTGCCAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGCCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCAt                                                                           >  2:203066/1‑140 (MQ=255)                                                            ttGCCAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGCCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGAc                                                                     <  1:174422/140‑1 (MQ=255)                                                                   ttGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGCCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCg                                                              >  1:379847/1‑140 (MQ=255)                                                                     gCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGCCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCggg                                                            >  1:287307/1‑140 (MQ=255)                                                                                   tcCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGCCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCA‑GTAGACATGTTCGGGGtt                                                         <  2:322060/128‑1 (MQ=255)                                                                                   tcCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGCCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCA‑GTAGACATGTTCGGGGtt                                                         >  1:322060/1‑128 (MQ=255)                                                                                        ctgcGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGCCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGa                                         >  1:146510/1‑140 (MQ=255)                                                                                                          ggAGAGGATCAGTGCGGTCGGGCCGCCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGt                       <  2:287307/140‑1 (MQ=255)                                                                                                            agagGATCAGTGCGGTCGGGCCGCCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGa                     >  1:210265/1‑140 (MQ=255)                                                                                                                     gTGCGGTCGGGCCGCCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCg            >  2:204978/1‑140 (MQ=255)                                                                                                                     gTGCGGTCGGGCCGCCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCg            >  1:210655/1‑140 (MQ=255)                                                                                                                      tGCGGTCGGGCCGCCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGt           >  1:323707/1‑140 (MQ=255)                                                                                                                        cGGTCGGGCCGCCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCt         <  2:210655/140‑1 (MQ=255)                                                                                                                          gTCGGGCCGCCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTc       >  1:66068/1‑140 (MQ=255)                                                                                                                           tCGGGCCGCCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCg      >  1:239333/1‑140 (MQ=255)                                                                                                                               gccgccCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCa  <  2:19740/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                        GAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCA  >  NC_000913/3079974‑3080239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑