Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,106,772	T→C	T353T (ACT→ACC)	nupG →	nucleoside:H(+) symporter NupG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,106,772	0	T	C	100.0%	86.8 / NA	24	T353T (ACT→ACC)	nupG	nucleoside:H(+) symporter NupG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/12);  total (12/12)

TACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGG  >  NC_000913/3106639‑3106896                                                                                                                                      |                                                                                                                             tactgCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACCAACGGc                                                                                                                        >  1:169773/1‑140 (MQ=255)      ctgGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACCAACGGCTTCgg                                                                                                                   >  2:104220/1‑140 (MQ=255)           aCTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACCAACGGCTTCGGCt                                                                                                                 >  1:161256/1‑137 (MQ=255)           aCTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACCAACGGCTTCGGCt                                                                                                                 <  2:161256/137‑1 (MQ=255)              gTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGACGACCAACGGCTTCGGCTGTATcc                                                                                                           >  1:382731/1‑140 (MQ=255)               tCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACCAACGGCTTCGGCTGTATCCt                                                                                                          >  2:238446/1‑140 (MQ=255)                   tgatCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACCAACGGCTTCGGCTGTATCCTcggc                                                                                                      >  1:272705/1‑140 (MQ=255)                         tttACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACCAACGGCTTCGGCTGTATCCTCGGCGGCATc                                                                                                >  1:354626/1‑140 (MQ=255)                               gTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACCAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGc                                                                                          <  2:27012/140‑1 (MQ=255)                                   cgcATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACCAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTa                                                                                      <  2:272705/140‑1 (MQ=255)                                      aTTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACCAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAg                                                                                   <  1:238446/140‑1 (MQ=255)                                         cGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACCAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAgttg                                                                                <  2:363302/140‑1 (MQ=255)                                             ttcttcAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACCAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTga                                                                            <  1:218838/140‑1 (MQ=255)                                                          ctGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACCAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCaa                                                               >  2:337468/1‑140 (MQ=255)                                                                   tgtTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACCAACGGCTTCGGCTGTATCCTCGGCGGCATGGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCagt                                                      >  2:388222/1‑138 (MQ=255)                                                                      ttGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACCAACGGCTTCGGATGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTAcc                                                   <  2:121333/140‑1 (MQ=255)                                                                           gAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACCAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTg                                              <  1:104220/140‑1 (MQ=255)                                                                                                  tCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACCAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCg                       <  2:354626/140‑1 (MQ=255)                                                                                                      gcCAGTGCACAAGGGATGTTCCTGATGATGACCAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTgg                   >  2:410164/1‑140 (MQ=255)                                                                                                           tGCACAAGGGATGTTCCTGATGATGACCAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGt                  >  1:367179/1‑136 (MQ=255)                                                                                                           tGCACAAGGGATGTTCCTGATGATGACCAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGt                  <  2:367179/136‑1 (MQ=255)                                                                                                              acaAGGGATGTTCCTGATGATGACCAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCg           <  1:344670/140‑1 (MQ=255)                                                                                                              acaAGGGATGTTCCTGATGATGACCAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCg           <  1:337468/140‑1 (MQ=255)                                                                                                                       gTTCCTGATGATGACCAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTgg  >  2:140163/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                             TACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGG  >  NC_000913/3106639‑3106896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑