Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,268,029	T→C	V59A (GTA→GCA)	yhaB →	protein YhaB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,268,029	0	T	C	100.0%	144.8 / NA	40	V59A (GTA→GCA)	yhaB	protein YhaB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/19);  total (21/19)

CCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAATATGAGTCCATTCATTATAGATATTTCTACAGGAGAACATGATGATTATATCATACAA  >  NC_000913/3267899‑3268162                                                                                                                                   |                                                                                                                                      ccAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGCATTATCTGc                                                                                                                              >  1:400208/1‑140 (MQ=255)      gCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGCATTATCTGCAATaa                                                                                                                         >  2:34072/1‑140 (MQ=255)             gTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGCATTATCTGCAATAACTGCAtt                                                                                                                  <  2:400208/140‑1 (MQ=255)               ggTTAACAATCACAATAGAAATATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGCATTATCTGc                                                                                                                              <  1:362774/126‑1 (MQ=255)               ggTTAACAATCACAATAGAAATATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGCATTATCTGc                                                                                                                              >  2:362774/1‑126 (MQ=255)                gTTAACAATCACAATAGAAATATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGCATTATCTGCAATAACTGCATTGaa                                                                                                               >  1:26630/1‑140 (MQ=255)                      aaTCACAATAGAAATATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGCATTATCTGCAATAACTGCATTGAATTTCTa                                                                                                         >  2:359323/1‑140 (MQ=255)                        tCACAATAGAAATATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGCATTATCTGCAATAACTGCATTGAATTTCTATc                                                                                                       >  2:281042/1‑140 (MQ=255)                        tCACAATAGAAATATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGCATTATCTGCAATAACTGCATTGAATTTCTATc                                                                                                       >  1:186172/1‑140 (MQ=255)                         cacaATAGAAATATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGCATTATCTGCAATAACTGCATTGAATTTCTATCa                                                                                                      <  2:69216/140‑1 (MQ=255)                         cacaATAGAAATATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGCATTATCTGCAATAACTGCATTGAATTTCTATCa                                                                                                      <  1:359323/140‑1 (MQ=255)                          acaATAGAAATATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGCATTATCTGCAATAACTGCa                                                                                                                    <  1:407140/125‑1 (MQ=255)                          acaATAGAAATATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGCATTATCTGCAATAACTGCa                                                                                                                    >  2:407140/1‑125 (MQ=255)                          acaATAGAAATATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGCATTATCTGCAATAACTGCATTGa                                                                                                                >  2:25524/1‑129 (MQ=255)                          acaATAGAAATATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGCATTATCTGCAATAACTGCATTGa                                                                                                                <  1:25524/129‑1 (MQ=255)                          acaATAGAAATATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGCATTATCTGCAATAACTGCATTGAATTTCTATCAt                                                                                                     >  1:242549/1‑140 (MQ=255)                          acaATAGAAATATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGCATTATCTGCAATAACTGCATTGAATTTCTATCAt                                                                                                     >  2:164448/1‑140 (MQ=255)                             aTAGAAATATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGCATTATCTGCAATAACTGCATTGAATTTCTATCATGGc                                                                                                  <  2:242549/140‑1 (MQ=255)                             aTAGAAATATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGCATTATCTGCAATAACTGCATTGAATTTCTATCATGGc                                                                                                  <  1:164448/140‑1 (MQ=255)                             aTAGAAATATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGCATTATCTGCAATAACTGCATTGAATTTCTATCATGGc                                                                                                  >  2:212232/1‑140 (MQ=255)                              tAGAAATATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGCATTATCTGCAATAACTGCATTGAATTTCTATCATGGCg                                                                                                 >  1:155835/1‑140 (MQ=255)                                  aaTATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGCATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGt                                                                                             <  1:281042/140‑1 (MQ=255)                                     atTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGCATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTcc                                                                                          <  1:219648/140‑1 (MQ=255)                                      ttGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGCATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCt                                                                                         >  2:82658/1‑140 (MQ=255)                                                     tAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGCATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGa                                                                          >  1:5693/1‑140 (MQ=255)                                                     tAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGCATTATCTGCAATAACTGCATTGAATTTCTATCATGGCAATGTTcc                                                                                          >  2:395502/1‑124 (MQ=255)                                                     tAAAAAGAAAGTTTGCAGAAATAGTCTCCGCCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGCATTATCTGCAATAACTGCATTGAATTTCTATCATGGCAATGTTcc                                                                                          <  1:395502/124‑1 (MQ=255)                                                            aaagTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGCATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAAc                                                                   <  1:329958/140‑1 (MQ=255)                                                            aaagTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGCATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAAc                                                                   <  1:170023/140‑1 (MQ=255)                                                                tttGCAGAGATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGCATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGa                                                               <  1:204483/140‑1 (MQ=255)                                                                          ttGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGCATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAATATGAGt                                                     <  2:5693/140‑1 (MQ=255)                                                                              ctcCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGCATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAATATGAGTCcat                                                 <  2:155835/140‑1 (MQ=255)                                                                                       tGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGCATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAATATGAGTCCATTCATTATAg                                        >  1:251847/1‑140 (MQ=255)                                                                                         aCGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGCATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAATATGAGTCCATTCATTATAGat                                      >  1:113424/1‑140 (MQ=255)                                                                                              aataaAATTTACAGTATAAGTAATGAACTGCGGAGAGCATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAATATGAGTCCATTCATTATAGATATTTc                                 >  2:1180/1‑140 (MQ=255)                                                                                                    aTTTACAGTATAAGTAATGAACTGCGGAGAGCATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAATATGAGTCCATTCATTATAGATATTTCTACAgg                           <  2:203730/140‑1 (MQ=255)                                                                                                              taAGTAATGAACTGCGGAGAGCATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAATATGAGTCCATTCATTATAGATATTTCTACAGGAGAACatgat                 <  2:253838/140‑1 (MQ=255)                                                                                                                       aaCTGCGGAGAGCATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAATATGAGTCCATTCATTATAGATATTTCTACAGGAGAACATGATGATTATATc        >  2:306944/1‑140 (MQ=255)                                                                                                                           gCGGAGAGCATTATCTGCAATAACTGCATTGATTTTCTATCATGGCGTTGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAATATGAGTCCATTCATTATAGATATTTCTACAGGAGAACATGAGGATTatataaaaa    >  2:226178/1‑135 (MQ=255)                                                                                                                            cGGAGAGCATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAATATGAGTCCATTCATTATAGATATTTCTACAGGAGAACATGATGATTATATCATACa   >  1:136210/1‑140 (MQ=255)                                                                                                                             ggAGAGCATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAATATGAGTCCATTCATTATAGATATTTCTACAGGAGAACATGATGATTATATCATACaa  <  1:406223/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                      CCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAATATGAGTCCATTCATTATAGATATTTCTACAGGAGAACATGATGATTATATCATACAA  >  NC_000913/3267899‑3268162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑