Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,273,769	A→G	V380A (GTT→GCT)	garP ←	galactarate/glucarate/glycerate transporter GarP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,273,769	0	A	G	100.0%	104.0 / NA	30	V380A (GTT→GCT)	garP	galactarate/glucarate/glycerate transporter GarP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/15);  total (15/15)

CATCCCACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGCCAATCACCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAACAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGCCAGCCCAGCGCACCAAATCCTTTGCCAAAGAAAGCCAGCGCCATCAGCATGACCACCAGCGTGGTGTTGTTGGTGTAGTTACATAAGATGATGGTGG  >  NC_000913/3273646‑3273901                                                                                                                            |                                                                                                                                     cATCCCACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGCCAATCACCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAGCAATCTCTTTCGgcgc                                                                                                                      <  2:388967/140‑1 (MQ=255)       aCGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGCCAATCA‑CAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAGCAATCTCTTTCGGCGCGGTGTCa                                                                                                               >  2:50033/1‑140 (MQ=255)                aGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGCCAATCACCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAGCAATCTCTTTCGGCGCGGTGTCAGAAATCAc                                                                                                       >  1:243012/1‑140 (MQ=255)                    ctgcATTGAAGGAGTGCAGTTCACTTACCAGGTAGCCAATCACCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAGCAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGc                                                                                                   <  1:374523/140‑1 (MQ=255)                             aGGAGTGCAGTTCACTTACCAGGTAGCCAATCACCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAGCAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGccagcccagc                                                                                          <  1:181962/140‑1 (MQ=255)                                            ttACCAGGTAGCCAATCACCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAGCAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGCCAGCCCAGCGCACCAAATCCTTTg                                                                           >  2:270342/1‑140 (MQ=255)                                                   gTAGCCAATCACCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAGCAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGCCAGCCCAGCGCACCAAATCCTTTGCCaaaga                                                                    >  2:172929/1‑140 (MQ=255)                                                       ccAATCACCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAGCAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGCCAGCCCAGCGCACCAAATCCTTTGCCAAAGAAAGc                                                                >  1:221527/1‑140 (MQ=255)                                                                  tGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAGCAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGCCAGCCCAGCGCACCAAATCCTTTGCCAAAGAAAGCCAGCGCCATCa                                                     >  2:237557/1‑140 (MQ=255)                                                                   ggAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAGCAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGCCAGCCCAGCGCACCAAATCCTTTGCCAAAGAAAGCCAGCGCCATCAg                                                    >  1:93298/1‑140 (MQ=255)                                                                     aGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAGCAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGCCAGCCCAGCGCACCAAATCCTTTGCCAAAGAAAGCCAGCGCCATCAGCa                                                  >  2:28287/1‑140 (MQ=255)                                                                      gTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAGCAATCTCTTTCGGCGCGGTGCCAGAAATCACCGGCCAGCCCAGCGCACCAAATCCTTTGCCAAAGAAAGCCAGCGCCATCAGCAt                                                 <  2:287190/140‑1 (MQ=255)                                                                       tGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAGCAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGCCAGCCCAGCGCACCAAATCCTTTGCCAAAGAAAGCCAg                                                             <  1:165274/127‑1 (MQ=255)                                                                       tGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAGCAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGCCAGCCCAGCGCACCAAATCCTTTGCCAAAGAAAGCCAg                                                             >  2:165274/1‑127 (MQ=255)                                                                       tGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAGCAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGCCAGCCCAGCGCACCAAATCCTTTGCCAAAGAAAGCCAGCGCCATCAGCATg                                                >  2:145293/1‑140 (MQ=255)                                                                         aCAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAGCAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGCCAGCCCAGCGCACCAAATCCTTTGCCAAAGAAAGCCAg                                                             <  1:305724/125‑1 (MQ=255)                                                                         aCAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAGCAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGCCAGCCCAGCGCACCAAATCCTTTGCCAAAGAAAGCCAg                                                             >  2:305724/1‑125 (MQ=255)                                                                          cAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAGCAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGCCAGCCCAGCGCACCAAATCCTTTGCCAAAGAAAGCCAGCGCCATCAGCATGacc                                             >  1:65015/1‑140 (MQ=255)                                                                                aGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAGCAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGCCAGCCCAGCGCACCAAATCCTTTGCCAAAGAAAGCCAGCGCCATCAGCAt                                                 >  2:261624/1‑130 (MQ=255)                                                                                aGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAGCAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGCCAGCCCAGCGCACCAAATCCTTTGCCAAAGAAAGCCAGCGCCATCAGCAt                                                 <  1:261624/130‑1 (MQ=255)                                                                                     aCATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAGCAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGCCAGCCCAGCGCACCAAATCCTTTGCCAAAGAAAGCCAGCGCCATCAGCATGACCACCAGCgtggt                                  <  2:221527/140‑1 (MQ=255)                                                                                         tGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAGCAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGCCAGCCCAGCGCACCAAATCCTTTGCCAAAGAAAGCCAGCGCCATCAGCATGACCACCAGCGTGGtgttg                              <  2:243012/140‑1 (MQ=255)                                                                                           ccAAAGACGTTAAAGACGCCGCCGCAGAGGCCAGCAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGCCAGCCCAGCGCACCAAATCCTTTGCCAAAGAAAGCCAGCGCCATCAGCATGACCACCAGCg                                      >  1:362080/1‑130 (MQ=255)                                                                                           ccAAAGACGTTAAAGACGCCGCCGCAGAGGCCAGCAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGCCAGCCCAGCGCACCAAATCCTTTGCCAAAGAAAGCCAGCGCCATCAGCATGACCACCAGCg                                      <  2:362080/130‑1 (MQ=255)                                                                                                     tAAAGACGCCGCCGCAGAGGCCAGCAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGCCAGCCCAGCGCACCAAATCCTTTGCCAAAGAAAGCCAGCGCCATCAGCATGACCACCAGCGTGGtgttgt                             <  1:32418/129‑1 (MQ=255)                                                                                                     tAAAGACGCCGCCGCAGAGGCCAGCAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGCCAGCCCAGCGCACCAAATCCTTTGCCAAAGAAAGCCAGCGCCATCAGCATGACCACCAGCGTGGtgttgt                             >  2:32418/1‑129 (MQ=255)                                                                                                     tAAAGACGCCGCCGCAGAGGCCAGCAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGCCAGCCCAGCGCACCAAATCCTTTGCCAAAGAAAGCCAGCGCCATCAGCATGACCACCAGCGTGGTGTTGTTGGTGTAGTTa                  <  1:145293/140‑1 (MQ=255)                                                                                                        aGACGCCGCCGCAGAGGCCAGCAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGCCAGCCCAGCGCACCAAATCCTTTGCCAAAGAAAGCCAGCGCCATCAGCATGACCACCAGCGTGGTGTTGTTGGTGTAGTTACAt               <  1:31123/140‑1 (MQ=255)                                                                                                               gccgcAGAGGCCAGCAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGCCAGCCCAGCGCACCAAATCCTTTGCCAAAGAAAGCCAGCGCCATCAGCATGACCACCAGCGTGGTGTTGTTGGTGTAGTTACATAAgatga        <  1:253130/140‑1 (MQ=255)                                                                                                                     gagGCCAGCAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGCCAGCCCAGCGCACCAAATCCTTTGCCAAAGAAAGCCAGCGCCATCAGCATGACCACCAGCGTGGTGTTGTTGGTGTAGTTACATAAGATGAtggtgg  <  2:93298/140‑1 (MQ=255)                                                                                                                            |                                                                                                                                     CATCCCACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGCCAATCACCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAACAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGCCAGCCCAGCGCACCAAATCCTTTGCCAAAGAAAGCCAGCGCCATCAGCATGACCACCAGCGTGGTGTTGTTGGTGTAGTTACATAAGATGATGGTGG  >  NC_000913/3273646‑3273901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑