Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,525,668	T→C	D161G (GAC→GGC)	nudE ←	ADP‑sugar diphosphatase NudE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,525,668	0	T	C	100.0%	125.5 / NA	34	D161G (GAC→GGC)	nudE	ADP‑sugar diphosphatase NudE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/18);  total (16/18)

AATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTG  >  NC_000913/3525532‑3525803                                                                                                                                         |                                                                                                                                        aaTTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGCCtt                                                                                                                                      <  1:333936/140‑1 (MQ=255)     gCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGCCTTCCAg                                                                                                                                  >  1:15351/1‑140 (MQ=255)     gCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGCCTTCCAg                                                                                                                                  >  1:6668/1‑140 (MQ=255)                aTAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGCCTTCCAGCAAATCcatca                                                                                                                       >  1:6766/1‑140 (MQ=255)                     ccTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGCCTTCCAGCAAATCCATCATATgc                                                                                                                  >  1:297670/1‑140 (MQ=255)                              cATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGCCTTCCAGCAAATCCATCATATGCgccagcggc                                                                                                         >  2:229256/1‑140 (MQ=255)                                 tgtgCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGCCTTCCAGCAAATCCATCATATGCgccagcggccag                                                                                                      <  1:113871/140‑1 (MQ=255)                                 tgtgCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGCCTTCCAGCAAATCCATCATATGCgccagcggccag                                                                                                      <  2:15351/140‑1 (MQ=255)                                 tgtgCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGCCTTCCAGCAAATCCATCATATGCgccagcggccag                                                                                                      <  2:6766/140‑1 (MQ=255)                                  gtgCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGCCTTCCAGCAAATCCATCATATGCgccagcggccagc                                                                                                     <  2:297670/140‑1 (MQ=255)                                   tgCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGCCTTCCAGCAAATCCATCATATGCgccagcggccagcg                                                                                                    >  2:353729/1‑140 (MQ=255)                                               aCTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGCCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAg                                                                                        <  2:312467/140‑1 (MQ=255)                                               aCTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGCCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAg                                                                                        <  2:404611/140‑1 (MQ=255)                                                           tACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGCCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAg                                                                                        <  1:22214/128‑1 (MQ=255)                                                           tACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGCCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAg                                                                                        >  2:22214/1‑128 (MQ=255)                                                               cTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGCCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCg                                                                        <  1:416666/140‑1 (MQ=255)                                                               cTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGCCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCg                                                                        <  2:6668/140‑1 (MQ=255)                                                                  gCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGCCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGct                                                                                   <  1:16523/126‑1 (MQ=255)                                                                  gCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGCCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGct                                                                                   >  2:16523/1‑126 (MQ=255)                                                                    cccTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGCCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTc                                                                   >  1:125417/1‑140 (MQ=255)                                                                    cccTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGCCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTc                                                                   >  1:275140/1‑140 (MQ=255)                                                                            tttCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGCCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGAtt                                                           <  2:281116/140‑1 (MQ=255)                                                                                    aTTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGCCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTag                                                   <  1:353729/140‑1 (MQ=255)                                                                                    aTTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGCCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTag                                                   >  1:199063/1‑140 (MQ=255)                                                                                       cgcgCACGAGGAACAGCGC‑CTGACATTGCGCGCTTCATTGAAGTCAGGGCCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGAt                                               >  1:149226/1‑140 (MQ=255)                                                                                                 gAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGCCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTa                                      <  1:154441/140‑1 (MQ=255)                                                                                                    cAGCGCACTGCCATTGCGCGCTTCATTGAAGTCAGGGCCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCa                                   <  1:287329/140‑1 (MQ=255)                                                                                                          aCTGACATTGCGCGCTTCATTGAAGTCAGGGCCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGatat                             >  1:123941/1‑140 (MQ=255)                                                                                                                aTTGCGCGCTTCATTGAAGTCAGGGCCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCAttt                       >  2:8677/1‑140 (MQ=255)                                                                                                                     gcgcTTCATTGAAGTCAGGGCCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTg                  <  2:199063/140‑1 (MQ=255)                                                                                                                                aaGTCAGGGCCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGa       <  2:125417/140‑1 (MQ=255)                                                                                                                                     aGGGCCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGa       >  2:157094/1‑135 (MQ=255)                                                                                                                                     aGGGCCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGa       <  1:157094/135‑1 (MQ=255)                                                                                                                                     aGGGCCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTg  >  1:292760/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        AATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTG  >  NC_000913/3525532‑3525803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑