Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,659,898	A→G	K223E (AAA→GAA)	mdtE →	multidrug efflux pump membrane fusion protein MdtE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,659,898	0	A	G	100.0%	67.4 / NA	19	K223E (AAA→GAA)	mdtE	multidrug efflux pump membrane fusion protein MdtE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/12);  total (7/12)

GCGTCAGCGGGAAATCGTCGGTGACCGTCGGCGCACTCGTTACCGCTAATCAGGCAGATTCGCTGGTTACCGTACAACGTCTGGACCCGATTTATGTCGATCTCACGCAGTCGGTGCAAGATTTCTTACGCATGAAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTACAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTCCGACCCGACAGTGGATG  >  NC_000913/3659764‑3660024                                                                                                                                       |                                                                                                                               gCGTCAGCGGGAAATCGTCGGTGACCGTCGGCGCACTCGTTACCGCTAATCAGGCAGATTCGCTGGTTACCGTACAACGTCTGGACCCGATTTATGTCGATCTCACGCAGTCGGTGCAAGATTTCTTACGCATGgaagaa                                                                                                                           >  2:226111/1‑140 (MQ=255)  cGTCAGCGGGAAATCGTCGGTGACCGTCGGCGCACTCGTTACCGCTAATCAGGCAGATTCGCTGGTTACCGTACAACGTCTGGACCCGATTTATGTCGATCTCACGCAGTCGGTGCAAGATTTCTTACGCATGGaagaag                                                                                                                          <  1:226111/140‑1 (MQ=255)      aGCGGGAAATCGTCGGTGACCGTCGGCGCACTCGTTACCGCTAATCAGGCAGATTCGCTGGTTACCGTACAACGTCTGGACCCGATTTATGTCGATCTCACGCAGTCGGTGCAAGATTTCTTACGCATGGAAGAAGAGGt                                                                                                                      <  2:38830/140‑1 (MQ=255)      aGCGGGAAATCGTCGGTGACCGTCGGCGCACTCGTTACCGCTAATCAGGCAGATTCGCTGGTTACCGTACAACGTCTGGACCCGATTTATGTCGATCTCACGCAGTCGGTGCAAGATTTCTTACGCATGGAAGAAGAGGt                                                                                                                      >  2:28086/1‑140 (MQ=255)                                cgcACTCGTTACCGCTAATCAGGCAGATTCGCTGGTTACCGTACAACGTCTGGACCCGATTTATGTCGATCTCACGCAGTCGGTGCAAGATTTCTTACGCATGGAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTc                                                                                            >  1:362242/1‑140 (MQ=255)                                       gTTACCGCTAATCAGGCAGATTCGCTGGTTACCGTACAACGTCTGGACCCGATTTATGTCGATCTCACGCAGTCGGTGCAAGATTTCTTACGCATGGAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAg                                                                                     <  1:299065/140‑1 (MQ=255)                                       gTTACCGCTAATCAGGCAGATTCGCTGGTTACCGTACAACGTCTGGACCCGATTTATGTCGATCTCACGCAGTCGGTGCAAGATTTCTTACGCATGGAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAg                                                                                     <  1:314125/140‑1 (MQ=255)                                       gTTACCGCTAATCAGGCAGATTCGCTGGTTACCGTACAACGTCTGGACCCGATTTATGTCGATCTCACGCAGTCGGTGCAAGATTTCTTACGCATGGAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAg                                                                                     <  1:62206/140‑1 (MQ=255)                                                 aTCAGGCAGATTCGCTGGTTACCGTACAACGTCTGGACCCGATTTATGTCGATCTCACGCAGTCGGTGCAAGATTTCTTACGCATGGAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTa                                                                           <  2:295978/140‑1 (MQ=255)                                                      gCAGATTCGCTGGTTACCGTACAACGTCTGGACCCGATTTATGTCGATCTCACGCAGTCGGTGCAAGATTTCTTACGCATGGAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAg                                                                                     <  2:69044/125‑1 (MQ=255)                                                      gCAGATTCGCTGGTTACCGTACAACGTCTGGACCCGATTTATGTCGATCTCACGCAGTCGGTGCAAGATTTCTTACGCATGGAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAg                                                                                     >  1:69044/1‑125 (MQ=255)                                                                       cGTACAACGTCTGGACCCGATTTATGTCGATCTCACGCAGTCGGTGCAAGATTTCTTACGCATGGAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTACAGCTCAATCTGGAAAATGGTa                                                     <  1:220612/140‑1 (MQ=255)                                                                            aaCGTCTGGACCCGATTTATGTCGATCTCACGCAGTCGGTGCAAGATTTCTTACGCATGGAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTACAGCTCAATCTGGAAAATGGTAAACGc                                                >  2:231173/1‑140 (MQ=255)                                                                                tCTGGACCCGATTTATGTCGATCTCACGCAGTCGGTGCAAGATTTCTTACGCATGGAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTACAGCTCAATCTGGAAAATGGTAAACGCTACa                                            >  2:190378/1‑140 (MQ=255)                                                                                                               tCGGTGCAAGATTTCTTACGCATGGAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTACAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTccgaccc             >  2:157955/1‑140 (MQ=255)                                                                                                               tCGGTGCAAGATTTCTTACGCATGGAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTACAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTccgaccc             >  1:161904/1‑140 (MQ=255)                                                                                                                 ggTGCAAGATTTCTTACGCATGGAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTACAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTccgacccga           <  1:157955/140‑1 (MQ=255)                                                                                                                   tGCAAGATTTCTTACGCATGGAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTACAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTCCGACCCGACa         <  2:362242/140‑1 (MQ=255)                                                                                                                   tGCAAGATTTCTTACGCATGGAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTACAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTCCGACCCGACa         <  1:190378/140‑1 (MQ=255)                                                                                                                          tttCTTACGCATGGAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTACAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTCCGACCCGACAGTGGATg  <  2:161904/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                               GCGTCAGCGGGAAATCGTCGGTGACCGTCGGCGCACTCGTTACCGCTAATCAGGCAGATTCGCTGGTTACCGTACAACGTCTGGACCCGATTTATGTCGATCTCACGCAGTCGGTGCAAGATTTCTTACGCATGAAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTACAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTCCGACCCGACAGTGGATG  >  NC_000913/3659764‑3660024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑