Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,911,606	T→C	intergenic (‑81/+233)	pstS ← / ← glmS	phosphate ABC transporter periplasmic binding protein/L‑glutamine‑‑D‑fructose‑6‑phosphate aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,911,606	0	T	C	100.0%	99.5 / NA	27	intergenic (‑81/+233)	pstS/glmS	phosphate ABC transporter periplasmic binding protein/L‑glutamine‑‑D‑fructose‑6‑phosphate aminotransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/12);  total (15/12)

ATAAGGTCGCGGCGACAACAGTTGCGACGGTGGTACGCATAACTTTCATAATGTCTCCTGGGAGGATTCATAAAGCATTGTTTGTTGGCTACGAGAAGCAAAATAGGACAAACAGGTGACAGTTATATGTAAGGAATATGACAGTTTTATGACAGAGAGATAAAGTCTTCAGTCTGATTTAAATAAGCGTTGATATTCAGTCAATTACAAACATTAATAACGAAGAGATGACAGAAAAATTTTCATTCTGTGACAG  >  NC_000913/3911477‑3911732                                                                                                                                  |                                                                                                                               aTAAGGTCGCGGCGACAACAGTTGCGACGGTGGTACGCATAACTTTCATAATGTCTCCTGGGAGGATTCATAAAGCATTGTTTGTTGGCTACGAGAAGCAAAATAGGACAAACAGGTGACAGTTATATGCAAGGAATATg                                                                                                                      <  2:63726/140‑1 (MQ=255)     ggTCGCGGCGACAACAGTTGCGACGGTGGTACGCATAACTTTCATAATGTCTCCTGGGAGGATTCATAAAGCATTGTTTGTTGGCTACGAGAAGCAAAATAGGACAAACAGGTGACAGTTATATGCAAGGAATATGACAg                                                                                                                  >  1:276903/1‑140 (MQ=255)        cgcgGCGACAACAGTTGCGACGGTGGTACGCATAACTTTCATAATGTCTCCTGGGAGGATTCATAAAGCATTGTTTGTTGGCTACGAGAAGCAAAATAGGACAAACAGGTGACAGTTATATGCAAGGAATATGACAGttt                                                                                                               >  2:145523/1‑140 (MQ=255)        cgcgGCGACAACAGTTGCGACGGTGGTACGCATAACTTTCATAATGTCTCCTGGGAGGATTCATAAAGCATTGTTTGTTGGCTACGAGAAGCAAAATAGGACAAACAGGTGACAGTTATATGCAAGGAATATGACAGttt                                                                                                               >  2:333365/1‑140 (MQ=255)         gcggcgACAACAGTTGCGACGGTGGTACGCATAACTTTCATAATGTCTCCTGGGAGGATTCATAAAGCATTGTTTGTTGGCTACGAGAAGCAAAATAGGACAAACAGGTGACAGTTATATGCAAGGAATATGACAGtttt                                                                                                              >  1:115737/1‑140 (MQ=255)           ggcgACAACAGTTGCGACGGTGGTACGCATAACTTTCATAATGTCTCCTGGGAGGATTCATAAAGCATTGTTTGTTGGCTACGAGAAGCAAAATAGGACAAACAGGTGACAGTTATATGCAAGGAATATGACAGTTTTAt                                                                                                            <  2:200640/140‑1 (MQ=255)                         cGACGGTGGTACGCATAACTTTCATAATGTCTCCTGGGAGGATTCATAAAGCATTGTTTGTTGGCTACGAGAAGCAAAATAGGACAAACAGGTGACAGTTATATGCAAGGAATATGACAGTTTTATGACAGAGAGATaaa                                                                                              <  2:276903/140‑1 (MQ=255)                              gtggtACGCATAACTTTCATAATGTCTCCTGGGAGGATTCATAAAGCATTGTTTGTTGGCTACGAGAAGCAAAATAGGACAAACAGGTGACAGTTATATGCAAGGAATATGACAGTTTTATGACAGAGAGATAAAGTCtt                                                                                         <  1:333365/140‑1 (MQ=255)                                  tACGCATAACTTTCATAATGTCTCCTGGGAGGATTCATAAAGCATTGTTTGTTGGCTACGAGAAGCAAAATAGGACAAACAGGTGACAGTTATATGCAAGGAATATGACAGTTTTATGACAGAGAGATAAAGTCTTCAGt                                                                                     >  1:192328/1‑140 (MQ=255)                                  tACGCATAACTTTCATAATGTCTCCTGGGAGGATTCATAAAGCATTGTTTGTTGGCTACGAGAAGCAAAATAGGACAAACAGGTGACAGTTATATGCAAGGAATATGACAGTTTTATGACAGAGAGATAAAGTCTTCAGt                                                                                     <  2:327644/140‑1 (MQ=255)                                   aCGCATAACTTTCATAATGTCTCCTGGGAGGATTCATAAAGCATTGTTTGTTGGCTACGAGAAGCAAAATAGGACAAACAGGTGACAGTTATATGCAAGGAATATGACAGTTTTATGACAGAGAGATAAAGTCTTCAGTc                                                                                    <  2:138897/140‑1 (MQ=255)                                           cTTTCATAATGTCTCCTGGGAGGATTCATAAAGCATTGTTTGTTGGCTACGAGAAGCAAAATAGGACAAACAGGTGACAGTTATATGCAAGGAATATGACAGTTTTATGACAGAGAGATAAAGTCTTCAGTCTGATTTaa                                                                            >  2:179972/1‑140 (MQ=255)                                              tCATAATGTCTCCTGGGAGGATTCATAAAGCATTGTTTGTTGGCTACGAGAAGCAAAATAGGACAAACAGGTGACAGTTATATGCAAGGAATATGACAGTTTTATGACAGAGAGATAAAGTCTTCAGTCTGATTTAAATa                                                                         <  2:47119/140‑1 (MQ=255)                                              tCATAATGTCTCCTGGGAGGATTCATAAAGCATTGTTTGTTGGCTACGAGAAGCAAAATAGGACAAACAGGTGACAGTTATATGCAAGGAATATGACAGTTTTATGACAGAGAGATAAAGTCTTCAGTCTGATTTAAATa                                                                         <  1:145523/140‑1 (MQ=255)                                               cATAATGTCTCCTGGGAGGATTCATAAAGCATTGTTTGTTGGCTACGAGAAGCAAAATAGGACAAACAGGTGACAGTTATATGCAAGGAATATGACAGTTTTATGACAGAGAGATAAAGTCTTCAGTCTGATTTAAATaa                                                                        >  1:34238/1‑140 (MQ=255)                                                            gggAGGATTCATAAAGCATTGTTTGTTGGCTACGAGAAGCAAAATAGGACAAACAGGTGACAGTTATATGCAAGGAATATGACAGTTTTATGACAGAGAGATAAAGTCTTCAGTCTGATTTAAATAAGCGTTGATATTCa                                                           >  2:110914/1‑140 (MQ=255)                                                             ggaggaTTCATAAAGCATTGTTTGTTGGCTACGAGAAGCAAAATAGGACAAACAGGTGACAGTTATATGCAAGGAATATGACAGTTTTATGACAGAGAGATAAAGTCTTCAGTCTGATTTAAATAAGCGTTGATATTCAg                                                          >  2:256234/1‑140 (MQ=255)                                                                  aTTCATAAAGCATTGTTTGTTGGCTACGAGAAGCAAAATAGGACAAACAGGTGACAGTTATATGCAAGGAATATGACAGTTTTATGACAGAGAGATAAAGTCTTCAGTCTGATTTAAATAAGCGTTGATATTCAGTCAAt                                                     >  1:19826/1‑140 (MQ=255)                                                                      aTAAAGCATTGTTTGTTGGCTACGAGAAGCAAAATAGGACAAACAGGTGACAGTTATATGCAAGGAATATGACAGTTTTATGACAGAGAGATAAAGTCTTCAGTCTGATTTAAATAAGCGTTGATATTCAGTCAATTACa                                                 <  2:34238/140‑1 (MQ=255)                                                                      aTAAAGCATTGTTTGTTGGCTACGAGAAGCAAAATAGGACAAACAGGTGACAGTTATATGCAAGGAATATGACAGTTTTATGACAGAGAGATAAAGTCTTCAGTCTGATTTAAATAAGCGTTGATATTCAGTCAATTACa                                                 <  2:19826/140‑1 (MQ=255)                                                                      aTAAAGCATTGTTTGTTGGCTACGAGAAGCAAAATAGGACAAACAGGTGACAGTTATATGCAAGGAATATGACAGTTTTATGACAGAGAGATAAAGTCTTCAGTCTGATTTAAATAAGCGTTGATATTCAGTCAATTACa                                                 <  1:179972/140‑1 (MQ=255)                                                                      aTAAAGCATTGTTTGTTGGCTACGAGAAGCAAAATAGGACAAACAGGTGACAGTTATATGCAAGGAATATGACAGTTTTATGACAGAGAGATAAAGTCTTCAGTCTGATTTAAATAAGCGTTGATATTCAGTCAATTACa                                                 <  1:256234/140‑1 (MQ=255)                                                                            cATTGTTTGTTGGCTACGAGAAGCAAAATAGGACAAACAGGTGACAGTTATATGCAAGGAATATGACAGTTTTATGACAGAGAGATAAAGTCTTCAGTCTGATTTAAATAAGCGTTGATATTCAGTCAATTACAAACAtt                                           >  2:266137/1‑140 (MQ=255)                                                                            cATTGTTTGTTGGCTACGAGAAGCAAAATAGGACAAACAGGTGACAGTTATATGCAAGGAATATGACAGTTTTATGACAGAGAGATAAAGTCTTCAGTCTGATTTAAATAAGCGTTGATATTCAGTCAATTACAAACAtt                                           >  2:245610/1‑140 (MQ=255)                                                                              ttgtttgtTGGCTACGAGAAGCAAAATAGGACAAACAGGTGACAGTTATATGCAAGGAATATGACAGTTTTATGACAGAGAGATAAAGTCTTCAGTCTGATTTAAATAAGCGTTGATATTCAGTCAATTACAAACATtaa                                         >  2:281226/1‑140 (MQ=255)                                                                                                  gCAAAATAGGACAAACAGGTGACAGTTATATGCAAGGAATATGACAGTTTTATGACAGAGAGATAAAGTCTTCAGTCTGATTTAAATAAGCGTTGATATTCAGTCAATTACAAACATTAATAACGAAGAGATGACAGaaa                     >  2:116382/1‑140 (MQ=255)                                                                                                                     tGACAGTTATATGCAAGGAATATGACAGTTTTATGACAGAGAGATAAAGTCTTCAGTCTGATTTAAATAAGCGTTGATATTCAGTCAATTACAAACATTAATAACGAAGAGATGACAGAAAAATTTTCATTCTGTGACag  >  1:348395/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                               ATAAGGTCGCGGCGACAACAGTTGCGACGGTGGTACGCATAACTTTCATAATGTCTCCTGGGAGGATTCATAAAGCATTGTTTGTTGGCTACGAGAAGCAAAATAGGACAAACAGGTGACAGTTATATGTAAGGAATATGACAGTTTTATGACAGAGAGATAAAGTCTTCAGTCTGATTTAAATAAGCGTTGATATTCAGTCAATTACAAACATTAATAACGAAGAGATGACAGAAAAATTTTCATTCTGTGACAG  >  NC_000913/3911477‑3911732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑