Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,920,104	A→G	R100R (CGT→CGC)	atpH ←	ATP synthase F1 complex subunit delta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,920,104	0	A	G	100.0%	55.4 / NA	16	R100R (CGT→CGC)	atpH	ATP synthase F1 complex subunit delta
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/10);  total (6/10)

ATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCACGCAGGTGAATAAACTGCTCCAGAACATCCGGGAGCGCGTTAAGACGACCATTTTCAGCCATAACCCGAATCAGGTTCTGACCGTTTTCGTCCAGTTGCTCACCACAAACTGCGATAAACGACTCGGCGAGCGTT  >  NC_000913/3919972‑3920239                                                                                                                                     |                                                                                                                                        aTCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCATCCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGAAGCCTCACACACTGCGCGCAGGt                                                                                                                                  >  2:258396/1‑140 (MQ=255)       tttGCAATTCAGCTTAACTTTGCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCGCGCAGGt                                                                                                                                  <  2:111273/134‑1 (MQ=255)       tttGCAATTCAGCTTAACTTTGCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCGCGCAGGt                                                                                                                                  >  1:111273/1‑134 (MQ=255)        ttGCAATTCAGCTTAACTTTGCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCGCGCAGGTGAATaaa                                                                                                                           <  2:3442/140‑1 (MQ=255)                   cTTAACTTTGCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCGCGCAGGTGAATAAACTGCTCCAGaa                                                                                                                >  1:194198/1‑140 (MQ=255)                           tGCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCGCGCAGGTGAATAAACTGCTCCAGAACATCCggg                                                                                                        >  2:336694/1‑140 (MQ=255)                             cGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCGCGCAGGTGAATAAACTGCTCCAGAACATCCGGGAg                                                                                                      >  2:240265/1‑140 (MQ=255)                                     aCGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCGCGCAGGTGAATAAACTGCTCCAGAACATCCGGGAGCGCGTTaa                                                                                              <  2:47957/140‑1 (MQ=255)                                                             tttCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCGCGCAGGTGAATAAACTGCTCCAGAACATCCGGGAGCGCGTTAAGACGACCATTTTCAGCCATAAccc                                                                      <  1:62773/140‑1 (MQ=255)                                                             tttCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCGCGCAGGTGAATAAACTGCTCCAGAACATCCGGGAGCGCGTTAAGACGACCATTTTCAGCCATAAccc                                                                      <  2:150795/140‑1 (MQ=255)                                                                 gcgAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCGCGCAGGTGAATAAACTGCTCCAGAACATCCGGGAGCGCGTTAAGACGACCATTTTCAGCCATAACCCGAAt                                                                  <  2:194198/140‑1 (MQ=255)                                                                 gcgAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCGCGCAGGTGAATAAACTGCTCCAGAACATCCGGGAGCGCGTTAAGACGACCATTTTCAGCCATAACCCGAAt                                                                  <  2:229517/140‑1 (MQ=255)                                                                               aCTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCGCGCAGGTGAATAAACTGCTCCAGAACATCCGGGAGCGCGTTAAGACGACCATTTTCAGCCATAACCCGAATCATGTTCTGACCGt                                                    <  2:300073/140‑1 (MQ=255)                                                                                      gCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCGCGCAGGTGAATAAACTGCTCCAGAACATCCGGGAGCGCGTTAAGACGACCATTTTCAGCCATAACCCGAATCAGGTTCTGACCGTTTTCGTc                                             <  1:258396/140‑1 (MQ=255)                                                                                               gAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCGCGCAGGTGAATAAACTGCTCCAGAACATCCGGGAGCGCGTTAAGACGACCATTTTCAGCCATAACCCGAATCAGGTTCTGACCGTTTTCGTCCAGTTGCTc                                    <  1:240265/140‑1 (MQ=255)                                                                                                                       gCCTCACTCACGGCGCGCAGGTGAATAAACTGCTCCAGAACATCCGGGAGCGCGTTAAGACGACCATTTTCAGCCATAACCCGAATCAGGTTCTGACCGTTTTCGTCCAGTTGCTCACCACAAACTGCGATAAACGACTc            >  2:26405/1‑140 (MQ=255)                                                                                                                                 cGGCGCGCAGGTGAATAAACTGCTCCAGAACATCCGGGAGCGCGTTAAGACGACCATTTTCAGCCATAACCCGAATCAGGTTCTGACCGTTTTCGTCCAGTTGCTCACCACAAACTGCGATAAACGACTCGGCGAGCGtt  >  1:189761/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                        ATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCACGCAGGTGAATAAACTGCTCCAGAACATCCGGGAGCGCGTTAAGACGACCATTTTCAGCCATAACCCGAATCAGGTTCTGACCGTTTTCGTCCAGTTGCTCACCACAAACTGCGATAAACGACTCGGCGAGCGTT  >  NC_000913/3919972‑3920239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑