Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,961,591	A→G	E305E (GAA→GAG)	rep →	ATP‑dependent DNA helicase Rep

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,961,591	0	A	G	100.0%	79.3 / NA	22	E305E (GAA→GAG)	rep	ATP‑dependent DNA helicase Rep
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/12);  total (10/12)

GATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTATGGCGCGGAGCTAAAAGTATTAAGCGCGAATAACGAAGAACATGAGGCTGAGCGCGTTACTGGCGAGCTGATCGCCCATCACTTCGTCAATAAAACGCAGTACAAAG  >  NC_000913/3961466‑3961706                                                                                                                              |                                                                                                                    gATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAGCTGGGTTATGgcgc                                                                                                       <  2:29186/140‑1 (MQ=255)  aTTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAGCTGGGTTATGGcgcg                                                                                                      <  1:233440/140‑1 (MQ=255)     ttCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAGCTGGGTTATGGCGCGGAg                                                                                                   >  1:298674/1‑140 (MQ=255)        cGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAGCTGGGTTATGGCGCGGAGCTa                                                                                                <  2:248553/140‑1 (MQ=255)                        tAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAGCTGGGTTATGGCGCGGAGCTAAAAGTATTAAGCGCGa                                                                                <  2:314784/140‑1 (MQ=255)                                aGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAGCTGGGTTATGGCGCGGAGCTAAAAGTATTAAGCGCGAATAACgaa                                                                        <  2:218024/140‑1 (MQ=255)                                                 tCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAGCTGGGTTATGGCGCGGAGCTAAAAGTATTAAGCGCGAATAACGAAGAACATGAGGCTGAgcg                                                       <  2:298674/140‑1 (MQ=255)                                                 tCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAGCTGGGTTATGGCGCGGAGCTAAAAGTATTAAGCGCGAATAACGAAGAACATGAGGCTGAgcg                                                       >  2:323449/1‑140 (MQ=255)                                                  ccGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAGCTGGGTTATGGCGCGGAGCTAAAAGTATTAAGCGCGAATAACGAAGAACATGAGGCTGAgcgc                                                      >  1:287082/1‑140 (MQ=255)                                                             cTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAGCTGGGTTATGGCGCGGAGCTAAAAGTATTAAGCGCGAATAACGAAGAACATGAGGCTGAGCGCGTTACTGGCGa                                           <  2:287082/140‑1 (MQ=255)                                                                   gcggcgAACATCCTGATCGCCAATAACCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAGCTGGGTTATGGCGCGGAGCTAAAAGTATTAAGCGCGAATAACGAAGAACATGAGGCTGAGCGCGTTACTGGCGAGCTGAt                                     >  1:79065/1‑140 (MQ=255)                                                                      gcgAACATCCTGATCGCCAATAACCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAGCTGGGTTATGGCGCGGAGCTAAAAGTATTAAGCGCGAATAACGAAGAACATGAGGCTGAGCGCGTTACTAGCGAGCTGATCGc                                  >  1:62105/1‑140 (MQ=255)                                                                                     gCCAATAACCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAGCTGGGTTATGGCGCGGAGCTAAAAGTATTAAGCGCGAATAACGAAGAACATGAGGCTGAGCGCGTTACTGGCGAGCTGATCGCCCATCACTTCGTaaa                   >  1:155879/1‑140 (MQ=255)                                                                                            aCCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAGCTGGGTTATGGCGCGGAGCTAAAAGTATTAAGCGCGAATAACGAAGAACATGAGGCTGAGCGCGTTACTGGCGAGCTGATCGCCCATCACTTCGTCAATAAAACg            <  1:323449/140‑1 (MQ=255)                                                                                            aCCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAGCTGGGTTATGGCGCGGAGCTAAAAGTATTAAGCGCGAATAACGAAGAACATGAGGCTGAGCGCGTTACTGGCGAGCTGATCGCCCATCACTTCGTCAATAAAACg            >  2:363398/1‑140 (MQ=255)                                                                                            aCCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAGCTGGGTTATGGCGCGGAGCTAAAAGTATTAAGCGCGAATAACGAAGAACATGAGGCTGAGCGCGTTACTGGCGAGCTGATCGCCCATCACTTCGTAAATAAAACg            <  2:155879/140‑1 (MQ=255)                                                                                             cccGCACGTCTTTGAAAAGCGTCTGTTCTCCGAGCTGGGTTATGGCGCGGAGCTAAAAGTATTAAGCGCGAATAACGAAGAACATGAGGCTGAGCGCGTTACTAGCGAGCTGATCGCCCATCACTTCGTCAATAAAACGc           <  2:62105/140‑1 (MQ=255)                                                                                                 cACGTCTTTGAAAAGCGTCTGTTCTCCGAGCTGGGTTATGGCGCGGAGCTAAAAGTATTAAGCGCGAATAACGAAGAACATGAGGCTGAGCGCGTTACTGGCGAGCTGATCGCCCATCACTTCGTCAATAAAACg            >  1:6876/1‑135 (MQ=255)                                                                                                 cACGTCTTTGAAAAGCGTCTGTTCTCCGAGCTGGGTTATGGCGCGGAGCTAAAAGTATTAAGCGCGAATAACGAAGAACATGAGGCTGAGCGCGTTACTGGCGAGCTGATCGCCCATCACTTCGTCAATAAAACg            <  2:6876/135‑1 (MQ=255)                                                                                                      cTTTGAAAAGCGTCTGTTCTCCGAGCTGGGTTATGGCGCGGAGCTAAAAGTATTAAGCGCGAATAACGAAGAACATGAGGCTGAGCGCGTTACTGGCGAGCTGATCGCCCATCACTTCGTCAATAAAACGCAGTACAAAg  >  2:133859/1‑140 (MQ=255)                                                                                                      cTTCGAAAAGCGTCTGTTCTCCGAGCTGGGTTATGGCGCGGAGCTAAAAGTATTAAGCGCGAATAACGAAGAACATGAGGCTGAGCGCGTTACTGGCGAGCTGATCGCCCATCACTTCGTCAATaa                >  2:20802/1‑126 (MQ=255)                                                                                                      cTTCGAAAAGCGTCTGTTCTCCGAGCTGGGTTATGGCGCGGAGCTAAAAGTATTAAGCGCGAATAACGAAGAACATGAGGCTGAGCGCGTTACTGGCGAGCTGATCGCCCATCACTTCGTCAATaa                <  1:20802/126‑1 (MQ=255)                                                                                                                              |                                                                                                                    GATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTATGGCGCGGAGCTAAAAGTATTAAGCGCGAATAACGAAGAACATGAGGCTGAGCGCGTTACTGGCGAGCTGATCGCCCATCACTTCGTCAATAAAACGCAGTACAAAG  >  NC_000913/3961466‑3961706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑