Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,361,659	A→G	T92T (ACT→ACC)	cadC ←	DNA‑binding transcriptional activator CadC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,361,659	0	A	G	100.0%	126.0 / NA	34	T92T (ACT→ACC)	cadC	DNA‑binding transcriptional activator CadC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/16);  total (18/16)

TAAGACTGTGGGAGGGAGAATCTGTGGCAGGAACCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTGGTACAGTAGCGATATAGACAGGACTATCTTCATCATTATCTTTTAATGACTTACGTAGTTCTGAGATACTCTGCGTCACAACGTGATTGGTGACAATACTTCTCTTCCAGACATTATCGATAAGTTCATCC  >  NC_000913/4361523‑4361785                                                                                                                                         |                                                                                                                               aaaGACTGGGGGAGGGAAATTCTGTGGCAGGAACCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTGGTACGGTa                                                                                                                             <  2:324496/139‑1 (MQ=255)         tgGGAGGGAGAATCTGTGGCAGGAACCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTGGTACGGTAGCGAtata                                                                                                                     >  2:326606/1‑140 (MQ=255)           ggagggagAATCTGTGTCAGGAACCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTGGTACGGTAGCGATATAGa                                                                                                                   >  2:288985/1‑140 (MQ=255)                   aaTCTGTGGCAGGAACCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTGGTACGGTAGCGat                                                                                                                        >  2:346435/1‑127 (MQ=255)                   aaTCTGTGGCAGGAACCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTGGTACGGTAGCGat                                                                                                                        <  1:346435/127‑1 (MQ=255)                      cTGTGGCAGGAACCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTGGTACGGTAGCGATATAGACAGGACTATCt                                                                                                        <  1:326606/140‑1 (MQ=255)                              ggAACCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTGGTACGGTAGCGATATAGACAGGACTATCTTCATCAtt                                                                                                <  2:75724/140‑1 (MQ=255)                                           gTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTGGTACGGTAGCGATATAGACAGGACTATCTTCATCATTATCTTTTAATGAc                                                                                   >  1:244727/1‑140 (MQ=255)                                                gggggAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCCTCGCTGTACCAGATAACAGGCACCATTAATTTATAGCCGCGCTTTGGTACGGTAGCGATATAGACAGGACTATCTTCATCATTATCTTTTAATGACTTACg                                                                              <  2:199284/140‑1 (MQ=255)                                                   ggAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTGGTACGGTAGCGATATAGACAGGACTATCTTCATCATTATCTTTTAATGACTTACGTAg                                                                           >  2:303350/1‑140 (MQ=255)                                                   ggAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTGGTACGGTAGCGATATAGACAGGACTATCTTCATCATTATCTTTTAATGACTTACGTAg                                                                           <  2:207278/140‑1 (MQ=255)                                                   ggAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAAATTATAGCCGCGCTTTGGTACGGTAGCGATATAGACAGGACTATCTTCATCATTATCTTTTAATGACTTACGTAg                                                                           >  1:366407/1‑140 (MQ=255)                                                     aGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTGGTACGGTAGCGATATAGACAGGACTATCTTCATCATTATCTTTTAATGACTTACGTAg                                                                           >  2:160214/1‑138 (MQ=255)                                                     aGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTGGTACGGTAGCGATATAGACAGGACTATCTTCATCATTATCTTTTAATGACTTACGTAg                                                                           <  1:160214/138‑1 (MQ=255)                                                     aGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTGGTACGGTAGCGATATAGACAGGACTATCTTCATCATTATCTTTTAATGACTTACGTAGtt                                                                         <  2:345570/140‑1 (MQ=255)                                                     aGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTGGTACGGTAGCGATATAGACAGGACTATCTTCATCATTATCTTTTAATGACTTACGTAGtt                                                                         >  1:345195/1‑140 (MQ=255)                                                     aGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTGGTACGGTAGCGATATAGACAGGACTATCTTCATCATTATCTTTTAATGACTTACGTAGtt                                                                         >  2:206033/1‑140 (MQ=255)                                                       gCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTGGTACGGTAGCGATATAGACAGGACTATCTTCATCATTATCTTTTAATGACTTACGTAGTTCt                                                                       >  1:379898/1‑140 (MQ=255)                                                        cGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTGGTACGGTAGCGATATAGACAGGACTATCTTCATCATTATCTTTTAATGACTTACGTAGTTCTg                                                                      >  1:82726/1‑140 (MQ=255)                                                                               ccTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTGGTACGGTAGCGATATAGACAGGACTATCTTCATCATTATCTTTTAATGACTTACGTAGTTCTGAGATACTCTGCGTCACAACGTGa                                               <  2:263541/140‑1 (MQ=255)                                                                                           tGTACCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTGGTACGGTAGCGATATAGACAGGACTATCTTCATCATTATCTTTTAATGACTTACGTAGTTCTGAGATACTCTGCGTCACAACGTGATTGGTGACAATa                                   >  2:388860/1‑140 (MQ=255)                                                                                             tACCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTGGTACGGTAGCGATATAGACAGGACTATCTTCATCATTATCTTTTAATGACTTACGTAGTTCTGAGATACTCTGCGTCACAACGTGATTGGTGACAATACt                                 >  2:165792/1‑140 (MQ=255)                                                                                              aCCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTGGTACGGTAGCGATATAGACAGGACTATCTTCATCATTATCTTTTAATGACTTACGTAGTTCTGAGATACTCTGCGTCACAACGTGATTGGTGACAATACtt                                <  2:119507/140‑1 (MQ=255)                                                                                                     aaCCGGCACCATTAATTTATAGCCGCGCTTTGGTACGGTAGCGATATAGACAGGACTATCTTCATCATTATCTTTTAATGACTTACGTAGTTCTGAGATACTCTGCGTCACAACGTGATTGGTGACAATACTTCTCTTcc                         >  1:241771/1‑140 (MQ=255)                                                                                                     aaCCGGCACCATTAATTTATAGCCGCGCTTTGGTACGGTAGCGATATAGACAGGACTATCTTCATCATTATCTTTTAATGACTTACGTAGTTCTGAGATACTCTGCGTCACAACGTGATTGGTGACAATACTTCTCTTcc                         >  1:418444/1‑140 (MQ=255)                                                                                                       ccGGCACCATTAATTTATAGCCGCGCTTTGGTACGGTAGCGATATAGACAGGACTATCTTCATCATTATCTTTTAATGACTTACGTAGTTCTGAGATACTCTGCGTCACAACGTGATTGGTGACAATACTTCTCTTCCAg                       >  1:328844/1‑140 (MQ=255)                                                                                                       ccGGCACCATTAATTTATAGCCGCGCTTTGGTACGGTAGCGATATAGACAGGACTATCTTCATCATTATCTTTTAATGACTTACGTAGTTCTGAGATACTCTGCGTCACAACGTGATTGGTGACAATACTTCTCTTCCAg                       >  1:225239/1‑140 (MQ=255)                                                                                                       ccGGCACCATTAATTTATAGCCGCGCTTTGGTACGGTAGCGATATAGACAGGACTATCTTCATCATTATCTTTTAATGACTTACGTAGTTCTGAGATACTCTGCGTCACAACGTGATTGGTGACAATACTTCTCTTCCAg                       >  1:200949/1‑140 (MQ=255)                                                                                                             ccATTAATTTATAGCCGCGCTTTGGTACGGTAGCGATATAGACAGGACTATCTTCATCATTATCTTTTAATGACTTACGTAGTTCTGAGATACTCTGCGTCACAACGTGATTGGTGACAATACTTCTCTTCCAGACATTa                 <  2:244727/140‑1 (MQ=255)                                                                                                                  aaTTTATAGCCGCGCTTTGGTACGGTAGCGATATAGACAGGAGCATCTTCATCATTATCTTTTAATGACTTACGTAGTTCTGAGATACTCTGCGTCACAACGTGATTGGTGACAATACTTCTCTTCCAGACATTATCGAt            <  2:389716/140‑1 (MQ=255)                                                                                                                     ttATAGCCGCGCTTTGGTACGGTAGCGATATAGACAGGACTATCTTCATCATTATCTTTTAATGACTTACGTAGTTCTGAGATACTCTGCGTCACAACGTGATTGGTGACAATACTTCTCTTCCAGACATTATCGATAAg         <  1:303350/140‑1 (MQ=255)                                                                                                                       ataGCCGCGCTTTGGTACGGTAGCGATATAGACAGGACTATCTTCATCATTATCTTTTAATGACTTACGTAGTTCTGAGATACTCTGCGTCACAACGTGATTGGTGACAATACTTCTCTTCCAGACATTATCGATAAGtt       <  2:328844/140‑1 (MQ=255)                                                                                                                          gCCGCGCTTTGGTACGGTAGCGATATAGACAGGACTATCTTCATCATTATCTTTTAATGACTTACGTAGTTCTGAGATACTCTGCGTCACAACGTGATTGGTGACAATACTTCTCTTCCAGACATTATCGATAAGTTCAt    <  2:366407/140‑1 (MQ=255)                                                                                                                            cgcgCTTTGGTACGGTAGCGATATAGACAGGACTATCTTCATCATTATCTTTTAATGACTTACGTAGTTCTGAGATACTCTGCGTCACAACGTGATTGGTGACAATACTTCTCTTCCAGACATTATCGATAAGTTCATcc  <  2:345195/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                               TAAGACTGTGGGAGGGAGAATCTGTGGCAGGAACCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTGGTACAGTAGCGATATAGACAGGACTATCTTCATCATTATCTTTTAATGACTTACGTAGTTCTGAGATACTCTGCGTCACAACGTGATTGGTGACAATACTTCTCTTCCAGACATTATCGATAAGTTCATCC  >  NC_000913/4361523‑4361785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑