Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,409,505	A→G	G77G (GGA→GGG)	rlmB →	23S rRNA 2'‑O‑ribose G2251 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,409,505	0	A	G	100.0%	62.1 / NA	18	G77G (GGA→GGG)	rlmB	23S rRNA 2'‑O‑ribose G2251 methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/10);  total (8/10)

AAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTT  >  NC_000913/4409369‑4409637                                                                                                                                         |                                                                                                                                     aaGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGGCGt                                                                                                                                   <  2:50657/140‑1 (MQ=255) aaGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGGCGt                                                                                                                                   <  1:403310/140‑1 (MQ=255)                   cGCTGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGGCGTCAGTATCAGGAAAACGAt                                                                                                                 <  2:269114/140‑1 (MQ=255)                       gATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGGCGTCAGTATCAGGAAAACGATCTGc                                                                                                             >  1:174385/1‑140 (MQ=255)                          tCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCTACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGGCGTCAGTATCAGGAAAACGATCTGc                                                                                                             <  2:103750/137‑1 (MQ=255)                          tCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCTACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGGCGTCAGTATCAGGAAAACGATCTGc                                                                                                             >  1:103750/1‑137 (MQ=255)                                               cGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGCGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGGCGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATc                                                                                     >  1:102387/1‑140 (MQ=255)                                                gTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGGCGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCa                                                                                    >  1:5794/1‑140 (MQ=255)                                                       tCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGGCGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTc                                                                             >  1:326009/1‑140 (MQ=255)                                                             tGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGGCGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCctgctg                                                                       >  1:407953/1‑140 (MQ=255)                                                                        cAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGGCGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACgg                                                            <  1:320801/140‑1 (MQ=255)                                                                                       aaaaGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGGCGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGca                                             <  2:174385/140‑1 (MQ=255)                                                                                       aaaaGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGGCGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGca                                             <  2:238476/140‑1 (MQ=255)                                                                                                  tGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGGCGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGgcg                                  <  2:219513/140‑1 (MQ=255)                                                                                                      gTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGGCGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCGTg                              <  2:407953/140‑1 (MQ=255)                                                                                                            cAGGGCATTATCGCCCGCGTGAAGCCAGGGCGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCg                        >  1:213541/1‑140 (MQ=255)                                                                                                                            gcgTGAAGCCAGGGCGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCa        >  2:199492/1‑140 (MQ=255)                                                                                                                                  aGCCAGGGCGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGtt  <  2:366666/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     AAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTT  >  NC_000913/4409369‑4409637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑