Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,431,450	T→C	T178T (ACA→ACG)	ytfE ←	iron‑sulfur cluster repair protein YtfE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,431,450	0	T	C	100.0%	127.7 / NA	37	T178T (ACA→ACG)	ytfE	iron‑sulfur cluster repair protein YtfE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/16);  total (21/16)

ACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCATTAATGCCGTTATACATCGCTTTCCAGGTGGTGCAGGCTTCTGGCGGCGGTGTGACGTTATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCTTTCCATTACGCTGATTGGCCCCATTGCCTGGCTGCCCATGCCTTGTTTGATCATCGGGAAGAGGATCTGCTCTT  >  NC_000913/4431323‑4431586                                                                                                                                |                                                                                                                                         actcacCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCATTAATGCCGTTATACATCGCTTTCCAGGTGGTGCAGGCTTCTGGCGGCTGCGTGACGTTATtg                                                                                                                              >  2:416643/1‑140 (MQ=255)    cacCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCATTAATGCCGTTATACATCGCTTTCCAGGTGGTGCAGGCTTCTGGCGGCGGCGTGACGTTATtggtg                                                                                                                           <  2:397801/140‑1 (MQ=255)    cacCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCATTAATGCCGTTATACATCGCTTTCCAGGTGGTGCAGGCTTCTGGCGGCGGCGTGACGTTATtggtg                                                                                                                           <  2:357286/140‑1 (MQ=255)      cccGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCATTAATGCCGTTATACATCGCTTTCCAGGTGGTGCAGGCTTCTGGCGGCGGCGTGACGTTATTggtggt                                                                                                                         <  2:187359/140‑1 (MQ=255)               gcgcgTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCATTAATGCCGTTATACATCGCTTTCCAGGTGGTGCAGGC‑TCTGGCGGCGGCGTGACGTTATTGGTGGTGTGTTTAATc                                                                                                               <  1:221722/140‑1 (MQ=255)                     gggAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCATTAATGCCGTTATACATCGCTTTCCAGGTGGTGCAGGCTTCTGGCGGCGGCGTGACGTTATTGGTGGTGTGTTTAATCACTTc                                                                                                          >  1:19881/1‑140 (MQ=255)                             tACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCATTAATGCCGTTATACATCGCTTTCCAGGTGGTGCAGGCTTCTGGCGGCGGCGTGACGTTATTGGTGGTGTGTTTAATCACTTCCAGCAGtt                                                                                                  >  1:203272/1‑140 (MQ=255)                             tACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCATTAATGCCGTTATACATCGCTTTCCAGGTGGTGCAGGCTTCTGGCGGCGGCGTGACGTTATTGGTGGTGTGTTTAATCACTTCCAGCAGtt                                                                                                  >  1:215753/1‑140 (MQ=255)                               cATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCATTAATGCCGTTATACATCGCTTTCCAGGTGGTGCAGGCTTCTGGCGGCGGCGTGACGTTATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCg                                                                                                >  2:95729/1‑140 (MQ=255)                                       tCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCATTAATGCCGTTATACATCGCTTTCCAGGTGGTGCAGGCTTCTGGCGGCGGCGTGACGTTATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGc                                                                                               >  1:151382/1‑133 (MQ=255)                                       tCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCATTAATGCCGTTATACATCGCTTTCCAGGTGGTGCAGGCTTCTGGCGGCGGCGTGACGTTATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGc                                                                                               <  2:151382/133‑1 (MQ=255)                                       tCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCATTAATGCCGTTATACATCGCTTTCCAGGTGGTGCAGGCTTCTGGCGGCGGCGTGACGTTATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGCCCGCTTc                                                                                        >  1:118012/1‑140 (MQ=255)                                       tCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCATTAATGCCGTTATACATCGCTTTCCAGGTGGTGCAGGCTTCTGGCGGCGGCGTGACGTTATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGCCCGCTTc                                                                                        >  1:115600/1‑140 (MQ=255)                                                 tgtgATCCATCAGGTCATCAATCAGTTCATTAATGCCGTTATACATCGCTTTCCAGGTGGTGCAGGCTTCTGGCGGCGGCGTGACGTTATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCg                                                                              <  1:95729/140‑1 (MQ=255)                                                        cATCAGGTCATCAATCAGTTCATTAATGCCGTTATACATCGCTTTCCAGGTGGTGCAGGCTTCTGGCGGCGGCGTGACGTTATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCTTTCCa                                                                       <  2:215753/140‑1 (MQ=255)                                                           cAGGTCATCAATCAGTTCATTAATGCCGTTATACATCGCTTTCCAGGTGGTGCAGGCTTCTGGCGGCGGCGTGACGTTATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGCCCGCTTCATCGCGCTCGCTTTCCATTa                                                                    >  1:76467/1‑140 (MQ=255)                                                            aGGTCATCAATCAGTTCATTAATGCCGTTATACATCGCTTTCCAGGTGGTGCAGGCTTCTGGCGGCGGCGTGACGTTATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCTTTCCATTAc                                                                   >  2:242099/1‑140 (MQ=255)                                                             ggTCATCAATCAGTTCATTAATGCCGTTATACATCGCTTTCCAGGTGGTGCAGGCTTCTGGCGGCGGCGTGACGTTATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCTTTCCa                                                                       >  1:209188/1‑135 (MQ=255)                                                             ggTCATCAATCAGTTCATTAATGCCGTTATACATCGCTTTCCAGGTGGTGCAGGCTTCTGGCGGCGGCGTGACGTTATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCTTTCCa                                                                       <  2:209188/135‑1 (MQ=255)                                                             ggTCATCAATCAGTTCATTAATGCCGTTATACATCGCTTTCCAGGTGGTGCAGGCTTCTGGCGGCGGCGTGACGTTATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCTTTCCATTACg                                                                  <  1:242099/140‑1 (MQ=255)                                                                 atcaatcaGTTCATTAATGCCGTTATACATCGCTTTCCAGGTGGTGCAGGCTTCTGGCGGCGGCGTGACGTTATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCTTTCCATTACGCTGa                                                              >  2:108753/1‑140 (MQ=255)                                                                 atcaatcaGTTCATTAAGGCCGTTATACATCGCTTTCCAGGTGGTGCAGGCTTCTGGCGGCGGCGTGACGTTGTTGGTGGTGTGGTTAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCTTTCCATTACGCTGa                                                              >  2:375698/1‑140 (MQ=255)                                                                               tAATGCCGTTATACATCGCTTTCCAGGTGGTGCAGGCTTCTGGCGGCGGCGTGACGTTATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCTTTCCATTACGCTGATTGGCCCCATTGcc                                                >  2:206673/1‑140 (MQ=255)                                                                                  tGCCGTTATACATCGCTTTCCAGGTGGTGCAGGCTTCTGGCGGCGGCGTGACGTTATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCTTTCCATTACGCTGATTGGCCCCATTGCCTgg                                             <  2:115600/140‑1 (MQ=255)                                                                                    ccGTTATACATCGCTTTCCAGGTGGTGCAGGCTTCTGGCGGCGGCGTGACGTTATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCTTTCCATTACGCTGATTGGCCCCATTGCCTGGCt                                           >  2:173126/1‑140 (MQ=255)                                                                                    ccGTTATACATCGCTTTCCAGGTGGTGCAGGCTTCTGGCGGCGGCGTGACGTTATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCTTTCCATTACGCTGATTGGCCCCATTGCCTGGCt                                           >  1:327898/1‑140 (MQ=255)                                                                                      gTTATACATCGCTTTCCAGGTGGTGCAGGCTTCTGGCGGCGGCGTGACGTTATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCTTTCCATTACGCTGATTGGCCCCATTGCCTGGCTGc                                         <  1:206673/140‑1 (MQ=255)                                                                                        tataCATCGCTTTCCAGGTGGTGCAGGCTTCTGGCGGCGGCGTGACGTTATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCTTTCCATTACGCTGATTGGCCCCATTGCCTGGCTGccc                                       >  1:50106/1‑140 (MQ=255)                                                                                               cGCTTTCCAGGTGGTGCAGGCTTCTGGCGGCGGCGTGACGTTATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCTTTCCATTACGCTGATTGGCCCCATTGCCTGGCTGCCCATGCCtt                                >  2:80656/1‑140 (MQ=255)                                                                                                 cTTTCCAGGTGGTGCAGGCTTCTGGCGGCGGCGTGACGTTATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCTTTCCATTACGCTGATTGGCCCCATTGCCTGGCTGCCCATGCCTTGt                              >  2:155162/1‑140 (MQ=255)                                                                                                         gtggtgCAGGCTTCTGGCGGCGGCGTGACGTTATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCTTTCCATTACGCTGATTGGCCCCATTGCCTGGCTGCCCATGCCTTGTTTGatcat                      <  1:155162/140‑1 (MQ=255)                                                                                                            gtgCAGGCTTCTGGCGGCGGCGTGACGTTATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCTTTCCATTACGCTGATTGGCCCCATTGCCTGGCTGCCCATGCCTTGTTTGATCATCgg                   >  2:36246/1‑140 (MQ=255)                                                                                                              gCAGGCTTCTGGCGGCGGCGTGACGTTATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCTTTCCATTACGCTGATTGGCCCCATTGCCTGGCTGCCCATGCCTTGTTTGATCATCGGGa                 <  2:118012/140‑1 (MQ=255)                                                                                                                       tGGCGGCGGCGTGACGTTATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCTTTCCATTACGCTGATTGGCCCCATTGCCTGGCTGCCCATGCCTTGTTTGATCATCGGGAAGAGGATCt        >  1:144155/1‑140 (MQ=255)                                                                                                                          cggcggCGTGACGTTATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCTTTCCATTACGCTGATTGGCCCCATTGCCTGGCTGCCCATGCCTTGTTTGATCATCGGGAAGAGGATCTGct     <  2:327898/140‑1 (MQ=255)                                                                                                                          cggcggCGTGACGTTATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCTTTCCATTACGCTGATTGGCCCCATTGCCTGGCTGCCCATGCCTTGTTTGATCATCGGGAAGAGGATCTGct     <  2:50106/140‑1 (MQ=255)                                                                                                                             cggCGTGACGTTATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCTTTCCATTACGCTGATTGGCCCCATTGCCTGGCTGCCCATGCCTTGTTTGATCATCGGGAAGAGGATCTGCtctt  <  1:173126/140‑1 (MQ=255)                                                                                                                                |                                                                                                                                         ACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCATTAATGCCGTTATACATCGCTTTCCAGGTGGTGCAGGCTTCTGGCGGCGGTGTGACGTTATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCTTTCCATTACGCTGATTGGCCCCATTGCCTGGCTGCCCATGCCTTGTTTGATCATCGGGAAGAGGATCTGCTCTT  >  NC_000913/4431323‑4431586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑