Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,585,793	C→T	R323R (AGG→AGA)	hsdR ←	type I restriction enzyme EcoKI endonuclease component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,585,793	0	C	T	100.0%	87.5 / NA	26	R323R (AGG→AGA)	hsdR	type I restriction enzyme EcoKI endonuclease component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/9);  total (17/9)

AGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGCCTGGCGGGAACGTCGATATTGTTACGTTTCGCCTCTACCACCGCGATGGGTTTGAGGCCGACAAACAGCACATAATCCGCAAAGCCCTGATTACCCGTTTCATCTTTTCCGGTCGGCCATTCGGCAATGGCTT  >  NC_000913/4585675‑4585926                                                                                                                       |                                                                                                                                      aGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGTCTGGCGGGAACGTCGATATTg                                                                                                                  >  2:393808/1‑140 (MQ=255)                ctGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGTCTGGCGGGAACGTCGATATTGTTACGTTTCGCctct                                                                                                   >  2:8279/1‑140 (MQ=255)                 tGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGTCTGGCGGGAACGTCGATATTGTTACGTTTCGCCTCTa                                                                                                  >  2:213868/1‑140 (MQ=255)                   gCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGTCTGGCGGGAACGTCGATATTGTTACGTTTCGCCTCTacc                                                                                                >  1:37401/1‑140 (MQ=255)                       tGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGTCTGGCGGGAACGTCGATATTGTTACGTTTCGCCTCTACCACcg                                                                                            >  1:222456/1‑140 (MQ=255)                          ttCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGTCTGGCGGGAACGTCGATATTGTTACGTTTCGCCTCTACCACCGCGa                                                                                         <  1:213868/140‑1 (MQ=255)                                   ttCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGTCTGGCGGGAACGTCGATATTGTTACGTTTCGCCTCTACCACCGCGATGGGTTTGa                                                                                >  1:40127/1‑140 (MQ=255)                                   ttCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGTCTGGCGGGAACGTCGATATTGTTACGTTTCGCCTCTACCACCGCGATGGGTTTGa                                                                                >  2:18399/1‑140 (MQ=255)                                              agtagtGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGTCTGGCGGGAACGTCGATATTGTTACGTTTCGCCTCTACCACCGCGATGGGTTTGAGGCCGACAAAc                                                                     <  2:37401/140‑1 (MQ=255)                                                      tCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGTCTGGCGGGAACGTCGATATTGTTACGTTTCGCCTCTACCACCGCGATGGGTTTGAGGCCGACAAACAGCACATa                                                             >  1:122885/1‑140 (MQ=255)                                                          gcaagGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGTCTGGCGGGAACGTCGATATTGTTACGTTTCGCCTCTACCACCGCGATGGGTTTGAGGCCGACaa                                                                       <  1:403571/126‑1 (MQ=255)                                                          gcaagGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGTCTGGCGGGAACGTCGATATTGTTACGTTTCGCCTCTACCACCGCGATGGGTTTGAGGCCGACaa                                                                       >  2:403571/1‑126 (MQ=255)                                                             agGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGTCTGGCGGGAACGTCGATATTGTTACGTTTCGCCTCTACCACCGCGATGGGTTTGAGGCCGACAAACAGCACATAATCCGCa                                                      <  1:8279/140‑1 (MQ=255)                                                             agGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGTCTGGCGGGAACGTCGATATTGTTACGTTTCGCCTCTACCACCGCGATGGGTTTGAGGCCGACAAACAGCACATAATCCGCa                                                      <  2:122885/140‑1 (MQ=255)                                                               gTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGTCTGGCGGGAACGTCGATATTGTTACGTTTCGCCTCTACCACCGCGATGGGTTTGAGGCCGACAAACAGCACATAATCCGCaaa                                                    >  1:269002/1‑140 (MQ=255)                                                                tttCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGTCTGGCGGGAACGTCGATATTGTTACGTTTCGCCTCTACCACCGCGATGGGTTTGAGGCCGACAAACAGCACATAATCCGCaaaa                                                   >  2:77112/1‑139 (MQ=255)                                                                tttCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGTCTGGCGGGAACGTCGATATTGTTACGTTTCGCCTCTACCACCGCGATGGGTTTGAGGCCGACAAACAGCACATAATCCGCAAAg                                                   <  1:271085/140‑1 (MQ=255)                                                                tttCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGTCTGGCGGGAACGTCGATATTGTTACGTTTCGCCTCTACCACCGCGATGGGTTTGAGGCCGACAAACAGCACATAATCCGCAAAg                                                   <  1:18399/140‑1 (MQ=255)                                                                  tCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGTCTGGCGGGAACGTCGATATTGTTACGTTTCGCCTCTACCACCGCGATGGGTTTGAGGCCGACAAACAGCACATAATCCGCAAAGcc                                                 <  2:174833/140‑1 (MQ=255)                                                                                              tACTGTAGCGATACGACTCATTGAGTCTGGCGGGAACGTCGATATTGTTACGTTTCGCCTCTACCACCGCGATGGGTTTGAGGCCGACAAACAGCACATAATCCGCAAAGCCCTGATTACCCGTTTCATCTTTTCCGGTc                     >  2:130131/1‑140 (MQ=255)                                                                                                     gCGATACGACTCATTGAGTCTGGCGGTAACGTCGATATTGTTACGTTTCGCCTCTACCACCGCGATGGGTTTGAGGCCGACAAACAGCACATAATCCGCAAAGCCCTGATTACCCGTTTCATCTTTTCCGGTCGGCCAtt              >  2:89091/1‑140 (MQ=255)                                                                                                     gCGATACGACTCATTGAGTCTGGCGGGAACGTCGATATTGTTACGTTTCGCCTCTACCACCGCGATGGGTTTGAGGCCGACAAACAGCACATAATCCGCAAAACCCTGATTACCCGTTTCATCTTTTCCGGTCGGCCAtt              <  1:77112/140‑1 (MQ=255)                                                                                                       gATACGACTCATTGAGTCTGGCGGGAACGTCGATATTGTTACGTTTCGCCTCTACCACCGCGATGGGTTTGAGGCCGACAAACAGCACATAATCCGCAAAGCCCTGATTACCCGTTTCATCTTTTCCGGTCGGCCATTCg            >  2:383252/1‑140 (MQ=255)                                                                                                        aTACGACTCATTGAGTCTGGCGGGAACGTCGATATTGTTACGTTTCGCCTCTACCACCGCGATGGGTTTGAGGCCGACAAACAGCACATAATCCGCAAAGCCCTGATTACCCGTTTCATCTTTTCCGGTCGGCCATTCgg           >  2:94832/1‑140 (MQ=255)                                                                                                               tCATTGAGTCTGGCGGGAACGTCGATATTGTTACGTTTCGCCTCTACCACCGCGATGGGTTTGAGGCCGACAAACAGCACATAATCCGCAAAGCCCTGATTACCCGTTTCATCTTTTCCGGTCGGCCATTCGGCAATGGc    >  1:79386/1‑140 (MQ=255)                                                                                                                 aTTGAGTCTGGCGGGAACGTCGATATTGTTACGTTTCGCCTCTACCACCGCGATGGGTTTGAGGCCGACAAACAGCACATAATCCGCAAAGCCCTGATTACCCGTTTCATCTTTTCCGGTCGGCCATTCGGCAATGGCtt  >  2:248091/1‑140 (MQ=255)                                                                                                                       |                                                                                                                                      AGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGCCTGGCGGGAACGTCGATATTGTTACGTTTCGCCTCTACCACCGCGATGGGTTTGAGGCCGACAAACAGCACATAATCCGCAAAGCCCTGATTACCCGTTTCATCTTTTCCGGTCGGCCATTCGGCAATGGCTT  >  NC_000913/4585675‑4585926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑